Self-assembling genes, vectors and uses thereof

ABSTRACT

The invention relates to a method for directing the self-assembly of a gene or gene assembly having three and preferably six or more fragments in a directionally and spatially ordered fashion to produce a gene, gene vector or large nucleic acid molecule. The method can be used to create libraries, such as combinatorial libraries. In another embodiment of the invention a vector is described for the incorporation and screeming of endogenous mouse promoter elements for the identification of cell-specific promoters.

This application claims the benefit of a provisional application U.S. Ser. No. 60/070,910, filed on Feb. 28, 1997, entitled “Self-Assembling Genes.”

FIELD OF THE INVENTION

This invention relates to the construction and usage of synthetic genes for genetic engineering and gene therapy.

BACKGROUND OF THE INVENTION

Recombination at the genetic level is important for generating diversity and adaptive change within genomes of virtually all organisms. Recombinant DNA technology is based upon simple ‘cut-and-paste’ methods for manipulating nucleic acid molecules in vitro. The pieces of genetic material, or DNA are first digested with a restriction endonuclease enzyme which recognizes specific sequences within the DNA. After preparation of two or more pieces of DNA, the ends of the DNA are further manipulated, if necessary, to make them compatible for ligation or joining together. DNA ligase, together with adenosine triphosphate (ATP) is added to the genes, ligating them back together. The genetic assembly containing an origin of DNA replication and a selectable gene is then inserted into a living cell, is grown up, and is positively selected to yield a pure culture capable of providing high yields of individual recombinant DNA molecules, or their products such as RNA or protein.

Significant improvements have been made to this technology over the last two and a half decades. Numerous enzymes, end-linkers and adapter molecules have been made commercially available, which facilitate in the construction of recombinant DNA molecules. By using two restriction enzymes with different single-stranded termini or blunt ends, it is possible to directionally assemble genes (forced cloning). This reduces the amount of screening required to determine orientation. Procedures have been automated for synthesis of single-stranded gene fragments up to 200 or more nucleotides in length by means of phosphoramidite chemistry, and the instrumentation is readily available through Applied Biosystems, Inc., Foster City, Calif. Such single-stranded fragments can be joined by annealing overlapping complimentary phosphorylated strands, and by enzymatically filling in the ends with DNA polymerase and DNA precursors In this way, multiple, overlapping, single-stranded fragments can be assembled into a larger, double-stranded superstructure. Whole genes have been synthesized by similar methods. However, it becomes increasingly difficult to use synthetic DNA strands when making genes larger than approximately one kilobase. Using gene amplification methods (e.g. polymerase chain reaction (PCR), Mullis et al., U.S. Pat. No. 4,683,195), together with synthetic oligonucleotides, it is possible to make biologically active, synthetic retro-vectors that are capable of RNA transcription, reverse-transcription, viral packaging, and integration into genomic DNA (see for example, Hodgson, WO94/20608). Hodgson, supra, also disclosed methods for cloning of transcriptional promoters into such a vector using traditional recombinant DNA technology.

Modified restriction enzyme sites, linkers, and adapters can change the primary or secondary structure of complex nucleic acid sequences thereby altering or obliterating a desired biological activity. For example, small mutations can drastically modify transcriptional promoters or change the reading frame of coding DNA. A logical goal of vectorology is to make exact constructs, without need of fortuitous restriction sites, adapters, or linkers.

Restriction endonucleases can be grouped based on similar characteristics In general there are three major types or classes: I, II (including IIS) and III. Class I enzymes cuts at a somewhat random site from the enzyme recognition sites (see Old and Primrose, 1994. Principles of Gene Manipulation. Blackwell Sciences, Inc., Cambridge, Mass., p.24). Most enzymes used in molecular biology are type II enzymes. These enzymes recognize a particular target sequence (i.e., restriction endonuclease recognition site) and break the polynucleotide chains within or near to the recognition site. The type II recognition sequences are continuous or interrupted. Class IIS enzymes (i.e., type IIS enzymes) have asymmetric recognition sequences. Cleavage occurs at a distance from the recognition site.

These enzymes have been reviewed by Szybalski et al. Gene 100:13-26, 1991. Class III restriction enzymes are rare and are not commonly used in molecular biology.

U.S. Pat. No. 4,293,652 employed a linker with a class IIS enzyme recognition sequence to permit synthesized DNA to be inserted into a vector without disturbing a recognition sequence. Brousseau et al. (Gene 17:279-289, 1982) and Urdea et al. (Proc. Natl. Acad. Sci. USA 80:7461-7465, 1983) disclose the use of class IIS enzymes for the production of vectors to produce recombinant insulin and epidermal growth factor respectively. Mandecki et al. described a method for making synthetic genes by cloning small oligonucleotides using a vector (Gene 68:101-107, 1988). Expansion of a population of oligonucleotides required synthesis, cloning excision and fragment purification. The oligonucleotides were used to create a complete plasmid.

Lebedenko et al. (Nucl. Acids Res. 19(24):6757-6761) illustrated the class IIS enzymes and PCR for precisely joining 3 nucleic acid molecules for convention sub-cloning using BamHI. Tomic et al. (Nucleic Acids Res., 18:1656, 1990), reported a method for site-directed mutagenesis using the polymerase chain reaction and class IIS enzymes to join two nucleic acid molecules. Two overlapping PCR primers were used where the primers included class IIS recognition sites. The primers included a region of complementarity to the template DNA and include one to a few site-directed mutations. Stemmer (U.S. Pat. No. 5,514,568) employed overlapping primers with class IIS enzymes to amplify a plasmid and to introduce specific mutations into DNA leaving all other positions unaltered.

There remains a need for the ordering and assembly of complex genes to overcome the problems associated with sequential sub-cloning such as multiple purification steps, the potential for sample loss, and the like. Moreover there is a need for eliminating the use of prokaryotic hosts and for minimizing or avoiding the risks associated with bacterial contamination resulting from the use of bacteria as intermediaries in the cloning process. Further, there remains a need for efficient methods to assemble large nucleic acid molecules or many-fragmented nucleic acid assemblies with precision.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1A. provides one schematic of six double stranded DNA fragments, each terminus comprising a unique overhanging two-nucleotide sequence complementary to only one other terminus

FIG. 1B. illustrates a three-piece ligation where 100% of the clones tested contained the predicted fragment order and desired fragment orientation.

FIG. 2. illustrates the use of a class IIS restriction endonuclease (as one example, Bpm 1), restriction endonuclease recognition site and the selection of cohesive overhanging ends (SEQ ID NOS:69-74).

FIGS. 3A-C illustrates an exemplary refrotransposon-derived vector including a murine VL30 LTR (NLV-3) and packaging signal, an internal ribosome entry site (IRES) from encephalomyocarditis virus (EMCV), a gene encoding a green fluorescent protein (GFP), additional internal VL30 sequences (solid bar), SV40 early region promoter and Tn5 aminoglycosidase phosphotransferase (neo) gene PBR322 plasmid origin of replication and a plus-strand primer binding site (VL30). An exemplary vector sequence is provided as VLBPGN (SEQ ID NO:1). FIG. 3B is an illustration of an LTR with the insertion of a U3 (transcriptional promoter)region rescued by reverse transcriptase-polymerase chain reaction (RT-PCR). The promoter is amplified from the RNA of a cell expressing the VL30 U3 region. Complementary overhanging ends are created using class IIS restriction endonuclease digestion sites within the LTR and within the promoter. FIG. 3C provides the linear structure of a VL30 RNA transcript from a mouse cell with a U3 region near the 3′-terminus of the RNA molecule. PCR primers include a class IIS enzyme recognition site to amplify the U3 region from the RNA resulting in a double stranded DNA molecule. Cleavage with a class IIS enzyme (here BpmI), results in a double-stranded DNA molecule with end complementary to a site in the vector of FIG. 3A.

FIGS. 4A-B is a schematic illustrating steps for assembling a combinatorial library of cis- or trans-acting nucleic acid sequences for assembly and screening, useful for the rescue of biologically active species. FIG. 4b is a diagram of a U3 (transcriptional enhancer and promoter region of an LTR illustrating several sub-divisions of the transcriptional control region, including a distal enhancer region, an enhancer repeat region, a medial promoter and a aproximal promoter. These regions have been described for other vectors in Hodgson et al. (1996. “Construction, Transmission and Expression of Synthetic VL30 Vectors” in Hodgson ed. Retro-vectors for Human Gene Therapy. R G Landes Company, Austin Tex.). Segments of these regions are amplified using primers for highly conserved sequences. Highly conserved sequences are determine based on a comparison of known VL30 sequences such as provided in FIG. 4.2 of Hodgson, 1996, infra). The parts are joined by annealing and ligation to provide an ordered assembly. Each construct is an allele or a representative of allelic variation in the combinatorial library.

FIG. 5 discloses two transcriptional promoters that have been rescued from mouse VL30 RNA sequences isolated from a mouse T-helper cell library. These promoters were assembled into a vector and introduced into ritroviral helper cells and packaged into recombinant retrovirus for introduction into human T-cells. After transduction to human T cells, a β-galactosidase reporter gene was expressed from the T cell-derived promoters. (SEQ ID NOS:2 and 3).

FIG. 6 discloses 10 biologically active mouse VL30 promoters obtained from mouse liver RNA. These promoters were introduced into the vector of SEQ ID NO:1. The vectors were introduced into retroviral helper cells and then packaged into retrovirus where they were introduced into human liver cells. The cells expressed the green fluorescent protein. (SEQ ID NOS:4-13).

FIG. 7 illustrates a similarity plot of nucleotide sequences found in VL30 U3 regions.

FIG. 8 illustrates a retro-vector comprising six double-stranded DNA fragments that were self-assembled into a circular structure using unique overlapping termini created using class IIS restriction endonucleases. Three templates and twelve primers were used in conjunction with three class IIS enzymes to make the six fragments that were ligated in a single step. The vector was efficiently self-assembled and was effectively transmitted by both DNA transfection as well as by retroviral transduction of the self-assembled DNA, without molecular cloning through a prokaryotic host (see Example 2).

BRIEF SUMMARY OF THE INVENTION

The invention described herein provides seamless, directional, ordered construction of complex DNA molecules, vectors and libraries. More particularly, it enables gene constructs to be assembled with greater efficiency and precision, and it enables multiple gene fragments to be assembled in the correct order and orientation without disturbing the internal structure of the gene. The method utilizes in vitro assembly of nucleic acid fragments and relies upon the unusual ability of certain enzymes to digest nucleic acid molecules at pre-determined sites without disrupting the structure of the gene. It is especially useful for the construction of genetic vectors for gene therapy or genetic engineering of cells and organisms. A particular application of the invention is in combinatorial, or evolutionary genetics, where it enables a large number of non-random, self-assembled constructs to be screened simultaneously for function.

In a preferred embodiment of this invention, the invention relates to a method method for assembling a gene or gene vector comprising the steps of: a) designing at least 6 primers to produce to amplify at least three fragments in at least three separate polymerase chain reactions wherein each primer comprises at least one predetermined restriction endonuclease recognition site that recognizes a restriction endonuclease that cleaves at a distance from the recognition site, a sequence complementary to a template nucleic acid for amplification, and bases positioned at the restriction endonuclease cleavage site that are selected to be complementary to only one other overhanging created from enzymatic cleavage of the fragments; b) combining the primers with template nucleic acid and performing the polymerase chain reaction to produce multiple copies of an amplified template fragment incorporating the restriction endonuclease recognition site; c) digesting the amplified template fragments with one or more restriction endonucleases that recognize the restriction endonuclease recognition site of the primers to create overhanging termini wherein each overhanging termini is complementary to only one other overhanging termini on another fragment; and d) combining the amplified and digested template fragments in a ligation reaction to produce a directionally ordered gene, nucleic acid fragment or gene vector.

In a preferred aspect of this embodiment, the restriction endonuclease is at least one class IIS restriction endonuclease and preferably, the class IIS restriction endonuclease is selected from the group consisting of: AlwI, Alw26I, BbsI, BbvI, BbvlI, BpmI, BsmAI, BsmI, BsmBI, BspMI, BsrI, BsrDI, Eco57I, EarI, FokI, GsuI, HgaI, HphI, MboII, MnlI, PleI, SapI, SfaNI, TaqII, Tth111II. Still more preferably, class II restriction endonuclease recognition sites (to be distinguished from class IIS restriction endonuclease recognition sites), linkers, or adapters are not used to create the gene or gene vector. In one embodiment, the product of the ligation reaction is introduced into prokaryotic or eukaryotic cells. Preferably, at least one template nucleic acid sequence is chosen from the group consisting of: transcriptional regulatory sequences; genetic vectors; introns and/or exons; viral encapsidation sequences; integration signals intended for introducing nucleic acid molecules into other nucleic acid molecules; retrotransposon(s); VL30 elements; or multiple allelic forms of a sequence.

In another preferred aspect of this embodiment, the method is used to generate combinatorial libraries of a target sequence. Preferably, the target sequence is part or all of a gene. In one embodiment, the gene encodes a protein. In one embodiment, the primers amplify allelic variants of part or all of a gene.

In still another preferred aspect of this embodiment, the product of the ligation reaction is passed between eukaryotic cells using a virus particle, by cell fusion, or by transfection. Preferably the product of the ligation reaction is not introduced into prokaryotic cells. Moreover, the method further comprises combining at least one screening or selection step to select the products of the ligation reaction. In one embodiment, the product of the ligation reaction is mutated during passage in cells in order to generate genetic diversity and preferably the product of the ligation reaction is mutated by homologous recombination during passage in cells.

In another aspect of this embodiment, the method is used to isolate and identify regulatory sequences from a cell. In anther aspect of this embodiment, cells containing the product of the ligation reaction are selected for enhanced biological activity. Preferably, the cells containing the product of the ligation reaction are selected for tissue-specific, hormone-specific or developmental-specific gene expression. Also preferably, the ligation reaction is a circularized gene vector.

In another embodiment of this invention, the invention relates to a nucleic acid primer having a 5′ and a 3′ end to amplify a nucleic acid fragment for the ligation of at least two fragments comprising: a restriction endonuclease recognition site that recognizes a restriction endonuclease, wherein the restriction endonuclease cleaves at a distance from the recognition site and creates overhanging termini; a sequence complementary to a template sequence to be amplified to produce the nucleic acid fragment; at least two nucleic acid bases positioned at the restriction endonuclease cleavage site and that form an overhanging terminus after cleavage by the restriction endonuclease, wherein the at least two nucleic acid bases are selected to be complementary to only one other overhanging terminus on another fragment of the ligation; and an affinity handle on the 5′ end of the primer. Preferably the primer further comprises an anchor to provide stability to the restriction enzyme at the restriction enzyme recognition site.

In yet another embodiment of this invention, the invention relates to a method for isolating and identifying promoters comprising the steps of: a) obtaining a vector comprising at least a portion of a promoter region from a retrovirus transposon LTR and having two non-complementary overhanging termini; b) designing at least two PCR primers to amplify at least one region of a retrovirus transposon LTR from template nucleic acid to produce at least one nucleic acid fragment wherein each primer comprises at least one predetermined restriction endonuclease recognition site that recognizes a restriction endonuclease that cleaves at a distance from the recognition site, a sequence complementary to a template sequence from a retrovirus transposon, and bases positioned at the restriction endonuclease cleavage site that are selected to be complementary to only one other overhanging terminus of the vector wherein the restriction endonuclease cleavage site is created from enzymatic cleavage of the fragments; b) combining the primers with template nucleic acid and performing a polymerase chain reaction to produce multiple copies of an amplified template fragment incorporating the restriction endonuclease recognition site; c) digesting the amplified template fragments with one or more restriction endonuclease that recognize the restriction endonuclease recognition site of the primer to create overhanging termini; and combining the amplified and digested template fragment in a ligation reaction with the vector to produce a gene vector with an intact LTR sequence. In one embodiment of this aspect of the invention, the template nucleic acid is DNA or RNA. In another embodiment of this aspect of the invention, the method further comprises the step of sequencing the insert to identify the promoter sequence. In one embodiment promoter sequences of SEQ ID NOS:1-13 identified using the methods of claim.

DETAILED DESCRIPTION OF THE INVENTION

In one embodiment of this invention, the invention relates to the seamless, oriented self-assembly of at least three DNA fragments having overlapping unique cohesive ends generated by the enzymatic cleavage of at least one restriction endonuclease that is capable of cleaving at a site distant to the restriction enzyme recognition site. Preferably the restriction endonucleases employed in this invention are class IIS restriction endonucleases. These enzymes recognize a predetermined group of nucleotides and cleave at a distance characteristic of the particular endonuclease from the recognition site. The term “unique cohesive ends” is used herein to refer to the notion that the cleavage site for the endonucleases of this invention can be manipulated to produce overhanging ends with unique termini selected by the investigator. The term “complementary” as used herein in reference to the overhanging ends of the fragments of this invention refers to standard complementarity recognized in the field of molecular biology. For example, the nucleotides sequence 5′-TAG-3′ is said to be complementary to the nucleotide sequence 5′-CTA-3′. The term “PCR” is used generally to refer to the polymerase chain reaction and its variations, including RT-PCR as well as other gene amplification techniques employing primers.

In a first step for practicing one embodiment of this invention, a series of at least three overlapping fragments are created trough the selection and creation of primers incorporating at least one class IIS restriction enzyme recognition sequence. The oligonucleotide primers of this invention are designed to amplify one or more nucleic acid fragments and comprise a sequence complementary to a target sequence for gene amplification, a recognition sequence for a restriction endonuclease that cleaves DNA at a distance from the recognition sequence (such as a class IIS restriction enzyme) and bases positioned at the restriction endonuclease cleavage site that are preferably unique and complementary to only one other overhanging termini in the annealing/ligation reaction that generates the complex nucleic acid molecules. Optionally, the primers of this invention can include an “affinity handle for cleanup” at the 5′ end. These sequences can be of any length, preferably at least about 6 bp and the sequences extend the primer in the 5′ direction from the restriction enzyme recognition site. This extra length gives many enzymes greater stability and improved activity. In addition, the sequence can be used for recognition and removal of the ends of the primers (either undigested fragments or digested ends of primers) using complementary nucleotide sequences bound to a solid support (such as cellulose, nitrocellulose or silica). Incubation with, or passage over a column or support containing the complementary sequences can be used to remote the tags by allowing them to anneal or hybridize. The nucleic acid can then be eluted from the column. Adapters can also be used in this invention. For purposes of this invention, adapters refer to double stranded fragments containing an enzyme recognition site, according to this invention. The adapters are ligated to double stranded DNA molecules, creating a Fragment analogous to a PCR fragment with similar sites derived from a primer. The primers or adapters can be prepared using a number of methods for synthesizing oligonucleotides known in the art. For example instruments for producing oligonucleotides are available from Applied Biosystems, Inc., Foster City, Calif.

In one example, for the design of an oligonucleotide primer for use in this invention, the particular complementary bases that will form the site for hybridization of the primer to template (i.e., target DNA or RNA) are selected. A restriction endonuclease recognition site is selected followed by a number of nucleotides to be positioned between the recognition site and the cleavage site. The nucleotides of the cleavage site are selected to include overhanging regions formed from the restriction endonuclease cleavage that are complementary to the overhanging regions of an adjacent fragment in the annealing/ligation reaction.

The length of the primer used in this invention can vary, but preferably the primer length is up to about 80 bases and preferably up to about 50 bases. In addition the primers are preferably at least about 15 bases in length and preferably at least about 25 bases in length. The 5′ region of the primer contains preferably at least about 6, preferably at least about 10 and still more preferably at least about 16-18 bases that are not complementary to the template DNA or RNA. Further, the primer incorporates a restriction endonuclease recognition site preferably 5′ to the region of complementarity and a restriction endonuclease digestion site preferably 5′ to the region of complementarity or within the region of complementarity. There are a variety of restriction endonucleases that cleave at a distance from the restriction endonuclease recognition site of a DNA strand and a variety of enzymes that are commercially available from New England Biolabs are provided in Table 1.

TABLE 1 Restriction endonucleases useful in the construction of self-assembling genes Site Distance Size size to of Overlap Enzyme (bp) overlap overlap type Alw26I 5  1-5 bp 4 bp 5′-Overhang BbsI 6  2-6 bp 4 bp 5′-overhang BpmI 6 16-14 bp 2 bp 3′-overhang BsmBI 6  1-5 bp 4 bp 5′-overhang BspMI 6  4-8 bp 4 bp 5′-overhang BsrDI 6  0-2 bp 2 bp 3′-overhang Eco57I 6 16-14 bp 2 bp 3′-overhang FokI 5  9-13 bp 4 bp 5′-overhang HgaI 5  5-10 bp 5 bp 5′-overhang HphI 5  8-7 bp 1 bp 3′-overhang MnlI 5  7-6 bp 1 bp 3′-overhang PleI 5  4-5 bp 1 bp 5′-overhang SapI 7  1-4 bp 3 bp 5′-overhang SfaNI 5  5-9 bp 4 bp 5′-overhang

In addition to the enzymes provided in Table 1, other restriction endonucleases that cleave at a distance from their restriction endonuclease recognition site include, but are not limited to, AlwI, BbsI, BbvI, BbvII, BsmAI, BsmI, BsrI, EarI, GsuI, MboII, TaqIl, Tth111II and their respective isoschizomers. These and other enzymes are known in the art and many are available from other manufacturers. The primers can be prepared to produce either 5′-overlapping ends or 3′-overlapping ends, as long as they are both are either 5′-overlapping ends or 3′-overlapping ends and are complementary to one other set of overlapping ends.

In the case of Bpm1 (see Example 1), the enzyme digests asymmetrically, 14-16 bp from the 3′-nucleotide of the recognition site. The resulting cleavage has a 3′-overhanging end of 2 bp. A second primer is then designed with a complementary overhanging end, and it is used to generate the adjoining fragment terminus. At the opposite ends of the two fragments that are to be joined, similar complementary, overhanging ends are designed.

The oligonucleotides are then combined with template nucleic acid (either DNA or RNA, e.g., such as for reverse transcriptase polymerase chain reaction (RT-PCR)) containing bases complementary to at least a 3′ portion of the primers (also referred to herein as “templates”). In one embodiment, the fragments are gene-amplified by PCR, RT-PCR or another gene amplification process using established PCR protocols such as those provided with PCR amplification kits, including those available from Perkin-Elmer Corp. (Emeryville, Calif.). Preferably, the PCR products are Analyzed by electrophoresis on a gel, such as an agarose gel and still more preferably the fragments of the predicted size are purified free of excess primers and small byproducts (such as by purification through a small column, such as a Qiagen™ column (Qiagen, Valencia, Calif.)). Following amplification or purification, the fragments are digested with the restriction endonuclease recognizing the restriction endonuclease recognition site in the primers. The digested fragments are then purified from the digested ends of the primers, preferably by preparative agarose gel electrophoresis. The fragments are combined, annealed and are ligated using standard hybridization and ligation conditions known for cloning (see Ausubel et al., Current Protocols in Molecular Biology, John Wiley & Sons, 1994).

FIG. 1A illustrates an example of a self-assembling gene construct (SEQ ID NO:1) comprising six fragments, each having unique overhanging dinucleotide ends. In this example, the ends of the fragments prepared by the methods of this invention are constructed using primers that include BpmI restriction endonuclease recognition sites It will be understood by those of ordinary skill in the art that one or more other restriction endonucleases (such as those of Table 1) could similarly be used for the self-assembling product of FIG. 1A. In a preferred embodiment, the primers were created as described above and preferably the 3′ends of the primers are non-palindromic (i.e., non self-complementary) to prevent self-annealing of such fragments. Each fragment in this example preferably joins to only one other dinucleotide overhang in the annealing/ligation mixture, assuring ligation only to the intended fragment partner. An advantage of this strategy is that the formation of concatamers or multimers is minimal. The restriction endonuclease site is removed by digestion with the restriction endonuclease, leaving the junction free of the extra DNA sequences associated with the site.

Using a single restriction endonuclease with a dinucleotide overhang (for example, using the enzyme BpmI) up to six pieces of genetic material can be joined together in a linear or circular form (such as a vector) without the need to perform sub-cloning procedures or detailed analysis of individual products because six unique combinations of dinucleotide overhangs create a directional clone with extremely high fidelity. With enzymes digesting single-base overlaps, only two fragments can be joined with positional and directional precision. With enzymes digesting three-base overlaps, 4³/2, or 32 fragments can be so joined in the correct order and orientation. Therefore, this invention also relates to the use of restriction endonuclease recognition sites that facilitate cleavage by restriction endonucleases with three-base overlaps and self-assembly gene constructs including 32 fragments. Alternatively, a combination of restriction endonuclease recognition sites for use with a combination of restriction enzymes that create two-base or three-base overlaps can be used. Each enzyme has its characteristic limits to self-assembly imposed by the size of the overlap. For example, there are sixteen dinucleotides, therefore BpmI fragments (which have two dinucleotide ends each) are limited to eight for the purpose of self-assembly; therefore in another embodiment of this invention an assembly comprising eight fragments is contemplated. However, four of the sixteen dinucleotides are palindromes. Use of these palindromic dinucleotides can create some infidelity in the annealing/ligation reaction. The enzyme HgaI has a five base overlap, and there are 1,024 pentanucleotide combinations, permitting 512 fragments to be ligated together directionally and in order (no palindromes). The fragments to be joined at a particular place are designed to have their cut sites aligned, so that the overlapping region fits together. In some cases, the target sequences will contain natural restriction endonuclease recognition sites for the enzyme that is being used, such as one or more internal BpmI sites. These sites have the potential to self-religate during vector or gene construction or they can be by passed by using a substitute enzyme in the primers (for example, Eco 571 can substitute for BpmI). Alternatively, these sites can be removed by site-directed mutagenesis after consideration to the, consequences of the mutagenized sequence to the gene or vector.

In addition to class IIS enzymes, class II restriction endonucleases can be used. These enzymes have intrinsic methylation activity that affects the outcome in either a negative or a positive way, depending on the purpose for which it is used. In a preferred embodiment, the methylation activity of class II enzymes is ablated by mutation or by genetic engineering to convert the enzyme to an effective class IIS enzyme to expand the repertoire of useful enzymes for this invention.

In another aspect of this invention, the primer design and target fragment sequence selection can be automated (see Example 5) using a computer to assist in the selection of unique overhanging ends that have complementarity only to the overhanging end of an adjacent fragment.

Therefore, this invention permits high-fidelity annealing and ligation of six or more fragments with unique overhanging termini complementary to a single other overhanging termini. Any multitude of combinations can be created by combining the type of overhanging termini that can be created. Moreover, if one is willing to sacrifice the fidelity of the reaction, a variety of combinations can be used to anneal a variety of fragment numbers. In these cases, some selection may be necessary, such as size selection of the resulting fragment based on electrophoretic migration or restriction endonuclease profiling, both methods well known to those of ordinary skill in the art

It is also necessary to have a high per-step efficiency (e.g., each step in the precess is performed with an efficiency of at least 80%) to effectively ligate large numbers of fragments without error. Where large numbers of fragments are used, the purity of the fragments becomes important. This means that for large numbers of fragments, the digested DNA fragments for annealing and ligation should be substantially pure. If undigested fragments, digested ends of primers, degraded or partially degraded molecules are present they can decrease the purity and affect the fidelity of the product. Therefore, it is particularly desirable to ensure complete digestion of both ends of each fragment and to remove al of the digested ends from the fragments prior to including the fragments in an annealing and ligation reaction. The use of Qiagen columns for oligonucleotide removal prior to digestion is generally sufficient to permit efficient digestion of the fragments. Agarose gel isolation is desirable after digestion particularly where the product contains some fragments that do not appear to be full length. The use of an analytical gel before and after digestion helps in determining whether both oligonucleotide tags have been removed. The isolation of fragments from agarose gels preferably avoids the use of ultraviolet light and exposure of the DNA to ethidium bromide is also preferably avoided. These methods can be avoided by running replicate lanes and staining only a portion of the gel.

The fragments and vector are then digested to yield fully complementary ends, and the fragments are preferably again purified, as described above (such as through a Qiagen column or by gel isolation). The purified fragments are ligated together in a test tube, under standard conditions, such as using bacteriophage T4 DNA ligase and ATP. Preferred ligations include at least 20 μg/ml total DNA concentration in the ligation mix to favor intermolecular interactions, and an equimolar ratio of fragments to be ligated. Where a prokaryotic intermediary is used, the ligated assemblage is transformed into a bacterium, such as an E. coli host, and the colonies are: selected with a drug (such as an ampicillin, tetracycline, or kanamycin marker). The colonies can then be selected either by individually selecting colonies or growing a mass culture, such as where a vector library has been created. Restriction enzyme analysis can be used to determine the identity of individual constructs or to assess the validation of the combination of plasmids. The plasmids can then be grown up and used as needed.

In one embodiment of this invention, at least a portion of a vector is used as one of the fragments for the ligation of at least three fragments according to this invention. In one example, where a vector is used as one of the starting fragments, two restriction endonuclease recognition sites recognizing an enzyme that cleaves at a distance from the recognition site, such as at least one BpmI site, can also be introduced into the vector. This permits the vector to be digested with the restriction endonuclease to produce a product having ends complementary to two ends of the insert DNA fragments. The vector can be made by amplifying a plasmid or portion thereof using the primers of this invention. Thus, the vector can also be constructed to include a variety of restriction endonuclease recognition sites using a variety of restriction endonucleases, including a variety of class II restriction endonucleases. In some cases, the target fragments for amplification will contain natural restriction endonuclease recognition sites for the enzyme that is being used for the self-assembly, such as for example, a fragment that includes one or more internal BpmI sites. Care should be taken either to utilize the complementarity of the naturally occurring site to reform the fragment as it originally existed or to eliminate the restriction endonuclease recognition site using, for example, site-directed mutagenesis. Preferably, the restriction endonuclease recognition site is be substituted for a different enzyme (in the case of BpmI. substituting Eco571 or BsrDI) that has an equivalent structure at its ends. Two or more fragments of insert or two or more fragments of vector with at least one insert are amplified using primers according to this invention.

The exemplary enzyme, BpmI digests DNA 14-16 base pairs (bp) from the 3′-nucleotide of the recognition sequence (RS). Thus, by placing the RS exactly 14-16 bp from the desired dinucleotide cut site, the practitioner tags the dinucleotide for ligation with another dinucleotide that is exactly complementary to it. Such a complementary dinucleotide can be inserted by using the same enzyme and RS to make another fragment which fits the first exactly, as illustrated in FIG. 1. Because there are sixteen possible dinucleotide combinations (including twelve combinations that do not have palindromic ends), it is possible to create up to six fragments with unique dinucleotides, and it is also possible to join them all together in a predetermined order and orientation (FIG. 1A). In addition, the palindromic sequences (such as AT, CG, TA, and GC) could also be used, although inefficiency and incorrect ligation will result from the self-complimentarity of these sequences. It is furthermore possible and desirable to have three or more fragments joined in this way, such that the construct is circular as in FIG. 1, comprising a vector that may be grown in a bacterial and/or eukaryotic host cell. If the genetic construct is to be used as a vector, the vector should be designed to include a proper origin of replication to enable it to replicate in a particular cell. For example, a prokaryotic origin of replication such as a coliform plasmid origin of replication enables circular DNAs to be propagated in E. coli host cells. It is desirable to have at least one selectable marker, such as a neomycin marker that enables recovery of the clone through a selection process. It is also desirable, but not essential, to have two or more selectable genetic elements, to permit dual selection. For example, if one of the fragments contains a prokaryotic plasmid origin of replication, and another fragment contains a selectable marker, then the two fragments are both selectable, since the construct will grow in prokaryotic cells in the presence of a selection drug (such as ampicillin) only when both fragments are present. Drug selection can be combined with the methods of directed self-assembly to assure a high percentage of correct products. Because of the unique complementarity of the fragments, each contributes a selectable element that leads to recovery of a high percentage of correct products.

For prokaryotic vector construction, at least one fragment should contain a prokaryotic origin of replication and one fragment should contain a drug resistance marker gene. However, an advantage of the methods of this invention is that the construct can be introduced directly into eukaryotic cells. Here no plasmid origin of replication is necessary and no prokaryotic selectable marker or other prokaryotic nucleic acid sequence is necessary. In cases where the vector is subject to regulatory approval or where optimal gene function is necessary, it may be undesirable to include prokaryotic sequences, such as extraneous plasmids or expressed prokaryotic fragments particularly if the sequences contain inmmunostimulatory sites that can lead to activation of the intracellular immune system and inactivation of a gene product (see Kreig, J. Lab. Clin. Med., 128:128-133, 1996) or to avoid risks of endotoxin contamination. Moreover, the use of self-assembled product, according to the methods of this invention saves labor and time involved in the screening process.

Thus, in a preferred embodiment of the invention, the nucleic acid fragments are self-assembled in vitro, and are transferred directly into eukaryotic cells, by transfection, injection, or other methods known in the art. In one embodiment the cells receiving the assembled product of this invention are helper cells for recombinant virus assembly (including, but not limited to retroviral helper cells for retroviral or retrotransposon vectors, adenovirus helper cells for adenovirus vectors or herpes simplex virus helper cells for herpes simplex vectors). Alternatively, the assembled product can be introduced into cells along with a helper virus or the assembled product can be introduced into target cells for direct expression. The assembled product can be a vector, a minichromosome vector, a portion of a chromosome, or the like. In the preferred case of a retroviral vector, the genes are first transfected into a first helper cell line (such as ecotropic helper cells, GP+E86 (Markowitz et al. J. Virol. 62:1120-1124, 1988). The retrovirus-containing supernatant from these cells is then filtered (0.45 mm Nalgene filters) preferably 48-72 hours after transfection and the filtrate is transferred to a second complementation retroviral helper cell line (such as PA317 retroviral helper cells, Miller et al., Mol. Cell. Biol. 6:2895-2902, 1986). After an additional 48 h, the second helper cell line is selected with the marker drug (such as the drug G418 for the selectable neomycin (neo) marker gene), until only drug-resistant cells remain. These cells contain stably integrated vectors that can be used to repeatedly transduce human cells. Advantageously, in the case of adenovirus vectors or other large eukaryotic-derived vectors including eukaryotic virus-derived vectors, it may be impossible to propagate them in prokaryotic hosts. The gene self-assembly method of the instant invention provides an alternative to in vitro recombination method of gene construction by permitting large constructs to be constructed.

One advantage of introducing the assembled product of this invention into a helper cell line to produce recombinant virus for the introduction of a gene or nucleic acid complex into a cell is that the assembled product will be auto-selected by the cells during the packaging process. Therefore, even where the overhanging termini have palindromic sequences, where there is more than one (but preferably less than four) unique complementary matches for a particular overhanging termini, or where concatamers have formed, only the correct or functional assembled products are expressed, transmitted, and assembled into virus. When the virus is then introduced into cells, the use of a reporter gene or another selectable marker provides yet a second layer of security for the selection of cells containing a properly assembled construct. For example, where a retrovirus helper cell line is used to produce a recombinant retrovirus containing the product of this invention (for retrovirus, RNA transcribed from the DNA product of the invention becomes packaged into the virus particle), a retrovirus-derived vector is transcribed as RNA and transmitted by packaging the RNA in a retrovirus particle. In order to be properly transmitted as a virus, the construct must be: 1) transcribed as RNA in a vector producer cell; 2) packaged into viral particles; 3) reverse transcribed into double-stranded DNA (in the recipient cell); and 4) integrated into the host chromosome. Each of these steps requires specific cis-acting sequences that must be correctly positioned within the vector. Thus, passage via retrovirus (or by other virus) is a means of auto-selection for the essential sequences.

In one application of the methods of this invention, the methods are used to rescue expressed sequences from RNA, or genomic sequences from cell DNA without disrupting the promoter sequences. Cellular transcriptional promoters are typically difficult to identify and isolate because they are generally not included in the RNA molecule and often extend over a considerable distance in a chromosome. One application of this invention relates to a promoter rescue technique that permits the entire promoter, or a fragment of a promoter to be isolated and cloned directly in to an expression vector without disruption of the flanking sequences. Promoter rescue techniques are known and include WO 94/20608 to Hodgson.

In a preferred embodiment of the invention, transcriptional promoters are cloned in a transcriptionally active manner for the selection and identification of new and/or of tissue or cell-specific promoters enabling them to be used, selected, or screened for activity directly. For example, FIG. 3 illustrates one example of the formation of a vector for the incorporation of promoter sequences and the ultimate identification of those sequences using an exemplary plasmid VLBPGN (SEQ ID NO:1) as provided in Example 3, with Bpm1 sites located within the locus of a retrotransposon (VL30) long terminal repeat (LTR). These methods preserve the structure and functionality of transcription factor response elements. The characteristic secondary structure of the LTR RNA remains very similar to the original LTR from which the promoter was rescued, thus preserving the important features of the original RNA/DNA molecule. Those of ordinary skill in the art will recognize that any of a variety of primers can be used with a variety of vectors and that the constructs of FIGS. 2 and 3 are exemplary and not limiting.

FIG. 2 illustrates the primers used to amplify the promoter insert (identified at a and c in FIG. 2), and the insert region of the LTR (boxed), both of which can be digested at the same nucleotide position with Bpm1, to ensure a proper and seamless fit. In this example, after digestion of the vector, the two Bpm1 sites leave non-complementary ends (a 3′-CC overhang on one end, and a 3′-GC overhang on the other). Thus, the ends will not efficiently anneal or ligate to one another. However, the complementary termini of the insert serves as linkage, enabling the plasmid to be completed by ligation.

In the example illustrated in FIG. 2, the terminus on the 3′-side (GC) is palindromic. Palindromic termini are self-complementary and can therefore ligate to themselves or to an identical terminus facing the opposite way (forming concatamers in the opposite direction). Despite the presence of palindromic termini and despite the potential for reduced fidelity in the self-assembling process, a large percentage of clones obtained by inserting promoter sequences into VLBPGN were assembled correctly (20/23). These levels are reduced somewhat when three or more fragments are combined for self-assembly, according to this invention and preferably, the use of palindromic termini are avoided when even numbers of nucleotides are exposed as overhanging termini because with even numbers of nucleotides there is an axis of symmetry. As noted above, where five base overhangs are used there are 1024 possible combinations of five nucleotides [(4)⁵], yet none of them is palindromic.

The vector of FIG. 3 is an example of a particular type of vector that is known as a retrotransposon vector. Retrotransposon vectors are described and reviewed in Hodgson et al., 1996 Retro-Vectors for Human Gene Therapy. R G Landes Company, Austin Tex., chapter 5 and see U.S. Pat. No. 5,354,674 to Hodgson. This type of vector is derived from a mouse cellular retro-transposon element that has no essential viral or cellular genes, and that has little sequence similarity to a retrovirus. However, this RNA (known as VL30 [virus-like, 30S]) has all the necessary cis-acting structural elements (such as LTRs and primer binding sites) required for efficient transmission by a type C murine or primate retrovirus. Thus, it is a parasite transmitted by retroviruses that is also expressed as a cellular RNA in most mouse cells and tissues. This RNA becomes packaged into retroviral particles when the mouse cells become infected by retrovirus. The retrovirus then transmits the VL30 (or a VL30 vector) to the next infected cell (which can be a human cell). The RNA is then reverse transcribed and integrated into the DNA of the host cell.

Some advantages of VL30 vectors (over retrovirus-derived vectors) are: 1) lack of viral genes and other sequence homology that could lead to replication competent retrovirus (RCR); 2) ability to be expressed long-term in vivo; 3) a variety of LTR transcriptional promoters that can be expressed in various tissues and under the influence of various hormones and other stimuli; and 4) the ability to express genes in a number of cell types that are targets of gene therapy. An additional advantage is that VL30 parts can be switched with those of classical retrovirus-derived vectors. For example, the LTR or packaging signal of VL30 can be used in place of the equivalent retroviral signal. The ability to make mixed, or chimeric retro-vectors is a special application of gene self assembly technology.

Using a specific primer set, such as that shown in FIG. 2, or others, as taught in this invention, it is possible to amplify the U3 sequences expressed in the RNA of many different types of mouse cells. This is done using standard RNA isolation methods (Ausubel et al., supra), coupled with extensive digestion with ribonuclease-free dexoyribonuclease, to eliminate residual DNA. Thus, to obtain a promoter that is expressed in the liver, one isolates RNA from liver and uses an RT-PCR procedure, such as those known in the art, with the primers to amplify the desired promoters. FIG. 6 illustrates liver RNA-derived promoters obtained using the methods of this invention. However, the promoters can also be derived by conventional PCR from cDNA libraries (FIG. 5 illustrates T cell-derived promoters that were obtained in this manner). It is also possible to use the well-known hormonal and pharmacological inducibility of VL30 LTRs to find LTRs that are responsive to peptides, hormones, and cytokines (for a table and description of VL30 pharmacologic responses (see Hodgson et al., 1996 Retro-Vectors for Human Gene Therapy. R G Landes Company, Austin Tex., chapter 4, and FIG. 4.2). Examples of substances inducing various VL30 promoters to high levels include: epidermal growth factor, basic fibroblast growth factor, insulin, erythropoietin, glucocorticoid hormones, activators of cyclic 3′-5′ AMP, and others. To rescue promoters with pharmacological responsiveness, cells or animals stimulated with the desired pharmacological agent are subjected to the RT-PCR procedure and the resulting U3 regions are cloned into a vector, (such as the exemplary VLBPGN) and are tested for inducibility. Standard RNA blotting procedures can be used before isolating VL30 promoters, to determine whether a particular drug or hormone causes induction of VL30 RNA expression in a particular mouse cell or tissue. After the promoter has been rescued, the vector is transmitted via retrovirus to the target cell (possibly a human equivalent of the mouse cell from which the promoter was rescued). After selection with the drug G418 (400-700 μg/ml, for 7-10 days) to select against cells not containing the vector, the target cell population is challenged with the pharmacological agent of choice. Reporter gene expression (in the example, GFP) or RNA expression, as determined by RNA blotting, can be used as an assay of gene inducibility by the agent (for exemplary gene expression methods, see Chakraborty et al., Biochem. Biophys Res. Commun. 209:677-683, 1995).

Using any specific primer set designed for use with VL30 retro-elements and using total cellular RNA from a particular mouse cell type as a template for RT-PCR, (using commercially available kits and methods therein) candidate promoter elements can be amplified. This method is useful for the identification of mouse-derived promoters and in particular the method is useful for the identification of cell-type specific or tissue-specific promoters from a mouse and for the selection of these promoters and the identification of tissue-specific or cell-specific promoters that function in human cells. Thus, these types of vectors and the methods for using these vectors permits the identification of promoters to permit controlled transcription of a foreign gene. The promoters, originally obtained from the mouse, can be used to effect tissue-specific or cell-specific expression in a human or animal liver cell such as a hepatocyte, or in a human blood cell such as a T-helper cell or in an erythrocyte (red blood cell). Methods are disclosed in Example 2 for the screening and selection of the promoters from a library of amplified promoter sequences. Other methods are well known to those of ordinary skill in the art. The specificity of the selected promoter can be assessed, for example, by introducing a selectable marker under the control of the test promoter in question and introducing this construct into various cells to assess the ability of the promoter to selectively regulate expression.

The amplified fragments represent U3 promoter regions from any RNA species expressed in the originating cells and their abundance will be in approximate proportion to the number of expressed copies of RNA in the original mixture. Example 3 illustrates one example using a mouse T-helper cell cDNA library to produce amplified fragments representing U3 regions expressed in T cells. The vectors were efficiently expressed as RNA and protein in PA317 helper cells, and were transmitted by retrovirus into human T-helper cells, where they were integrated and expressed as protein in the form of a β-galactosidase reporter gene, as visualized by X-gal staining. The products of this experiment are provided in FIG. 5 and as SEQ ID NOS: 2 and 3 from T-helper RNA. The products of another experiment are shown in FIG. 6 as SEQ ID NOS: 4-13 from mouse liver RNA (by RT-PCR).

Examination of the different U3 sequences isolated from T cells and from liver revealed several things. First, the T cell U3 sequences were related to each other, as were the liver sequences. However, the two types of U3 sequences were quite different between the two sources (T-cell, FIG. 5 and liver, FIG. 6). Specifically, the liver sequences (FIG. 6) appeared to be a closely related group, differing mostly by single point mutations, some of which may affect transcription factor binding sites. Some of the polymorphic sites included: a phorbol ester response element (VLTRE); a Rel/NFκb binding region, and a possible glucocorticoid response element (GRE). Some of these polymorphisms are illustrated in FIG. 6. The T cell-derived sequences (FIG. 5, SEQ ID NO:2 and 3), on the other hand, differed significantly in length, with SEQ ID NO:3 missing more than 120 bases (compared with SEQ ID NO:2) including putative binding sites for retinoids (RAR/RXR) and several elements contained within the enhancer repeat region (including a cAMP response element (VLCRE, or CREB/jun binding site), and putative serum response element (SRE, CARG, and NF1/IL6). SEQ ID NO:3 represented one out of five clones sequenced, while SEQ ID NO:2 represented four out of five. Possible sites of interactions between transcription factors and DNA can be observed by comparing the experimentally derived U3 sequences with those in Hodgson et al.,(Retro-Vectors for Human Gene Therapy, 1996 FIG. 4.2 supra). In addition to the deleted sequences of SEQ ID NO:2, there: are a number of single base differences within the conserved regions of the two T cell-derived sequences.

Advantageously, a number of new VL30 promoter sequences (SEQ ID NOS: 2-13, supra) were identified using these methods despite the fact that VL30 RNA comprises only about 0.3% of cell mRNA represented in a cDNA library. Moreover, in each case, the cloned insert was isolated without the need to use linkers, adapters, or multiple cloning sequences such as those that are typically use for other library construction methods. The promoter sequences can be used in the vectors disclosed here to express inserted foreign genes or the promoter sequences can be substituted into other retroviral vectors, such as MoMLV-derived vectors or other VL30-derived vectors. Further, vectors containing the promoter sequences can be propagated in retroviral helper cells, such as PA317 (U.S. Pat. No. 4,861,719 to Miller) or introduced into cells by chemical or physical transfection.

In another application of the methods of this invention, libraries of amplified sequences can be incorporated into vectors using two or more fragments and using the restriction endonucleases cleaving at a distance from their recognition sites. Preferably the vectors are created using six or more fragments and preferably greater than 10 or more fragments. For example, as applied to VL30 promoter sequences, because there are over a hundred VL30 retro-elements in the mouse genome, it is possible to amplify all of the promoter sequences en masse, and propagate them en masse, enabling screening by serial passage through helper cells (such as the PA317 helper cell line) or by means of a replication competent retrovirus, as illustrated in Examples 3 and 4. Conversely, the promoter region may be broken down into several sub-domains and permutations of each could be combined and screened to enhance the chances of generating a superior construct (FIG. 4B).

As an example of breaking a promoter region down into several sub-domains, FIG. 7 illustrates a similarity plot of nucleotide sequences found in VL30 U3 regions. Plot similarity was performed using the Plot Similarity program (Wisconsin Sequence Analysis Package, release 8.1, Genetics Computer Grout, Madison, Wis.). This program plots the running average of the similarity among the sequences in a multiple sequence alignment. The sequences compared were those found in FIG. 4.2 of Hodgson, 1996, chapter 4 (infra). That is, the plot discloses the degree of conservation of VL30 promoter sequences among known VL30 promoters. From the figure, it can be seen that conserved sequences (close to 100% conserved) can be used as primer binding sites to amplify the adjacent sequences by PCR. An allelic mixture of three fragment sets is then created to make a combinatorial library of promoters that can be positively selected, such by using retroviral amplification of the active sequences. This, used in combination with the FIG. 4.2 (Hodgson, 1996, chapter 4 supra) can be used to determine regions of high similarity. Regions of high similarity within the U3 region can be replaced with one another. Therefore, a library of permutations of these sections can be made by combining allelic pools obtained by amplifying the sequences from individual subsections, followed by ligating the subsections in the correct order using the methods of the instant invention for gene self-assembly. For example, sub-section 1 can include the distal enhancer (from the LTR 5′-end to the site of insert primer 2, see for example the region defined by the insert primers 1 and 2 (SEQ ID NOS 55 and 56 of Example 4). In this way, using a plot similarity (such as FIG. 7), within each sub-section, the primers position fragments within a region of nearly 100% identity. Degenerate primers can also be used in these experiments to account for multiple nucleic acid base combinations along a particular sequence. In each case, the primers preferably are designed to have a melting temperature that is compatible with the RT-PCR conditions being used, and the conditions should be those recommended by the manufacturer (preferably Perkin Elmer Corp., Emeryville, Calif.). In Example 4, a set of primers is given that can be used to amplify different U3 subsections, together with directions for assembling a combinatorial library.

It will be appreciated by persons of ordinary skill in the art that the methods of the instant invention can thus be used to make allelic libraries of a variety of genes. For example, different allelic portions of a gene can be combined in a predetermined order and orientation to produce combinatorial libraries, without the need for fortuitous restriction sites separating the parts in the original construct, and without perturbing the important sequences joining the parts using the methods of this invention.

In this invention primers are constructed as described above. However, for the generation of allelic libraries or more complex library constructs it may be helpful to include 5′tags into the 5′ end of the primer. The purposes of the tag sequence are: 1) to provide extra nucleotides on both sides of the restriction endonuclease recognition sites (for more efficient digestion); and 2) to enable recovery of sequence tags or undigested fragments by means of an affinity reagent (such as silica, magnetic beads, or nitro-cellulose containing the complementary sequences) for purification. The use of an affinity reagent permits the digested ends to be purified away from the digested fragments. Furthermore, if any undigested ends remain after thorough digestion, the affinity reagent will remove them, further aiding in the purification. In one embodiment, affinity purification of the digested fragments is used in place of gel isolation, eliminating possible damage caused by ultraviolet light as well as possible damage caused by dye (e.g., ethidium bromide) binding to the DNA.

It will also be appreciated that a number of other variations to the primer sequences can be employed. For example, as discussed above, the enzyme recognition site for an enzyme that digests outside of its recognition sequence is included in the primer, so that the DNA digest creates an overlapping end that is complementary to one other terminus to which it will be joined. The enzyme recognition site can be moved to any location within the primer so as to digest the DNA at the exact location desired. The primer can also be programmed with a novel enzyme recognition sequence to add any desired sequences between the two sequences to be joined or to incorporate a linker or adapter if desired. If the sequences to be amplified contain the enzyme recognition site of the primers, it may be necessary to switch to a different enzyme usage. The use of several different enzymes is possible and has been discussed above. As with other PCR procedures, after the initial primer selections have been made the primers are assessed for their ability to fold back on themselves or to create internal secondary structure. The primers are preferably modified to avoid palindromic sequences or the potential for self folding within a primer. Nucleic acid analytical software (such as the Wisconsin GCG package, Oxford Biomolecular, Oxford, UK) is available to perform this analysis and aid in the selection of alternative primers.

In addition, as with all PCR processes, it is necessary to determine the melting temperatures (T_(m)), and to adjust the annealing temperature of the PCR reactions to compensate for such temperatures. Finally, it is important to perform a sequence redundancy search, to determine whether the target sequence (the sequence complementary to the primer) is found more than once in the region to be amplified. If the sequence is repeated, it will be necessary to use a different primer in order to establish the single, correct priming site. Preferably, no more than 6-8 bases of incorrect target complementarity at the 3′-end of the complementary region is used and to allow a difference of at least 10° C. between the T_(m)s of the correct and the incorrect target. The annealing temperature should always be at least 5° C. lower than the T_(m) of the correct target and 5° C. above the T_(m) of the incorrect target. Again, the necessary software and instructions are readily available from the cited sources (Wisconsin Gene Computer Group and Oxford Biomolecular, supra)

Next, a vector is constructed to include the appropriate elements for expression in the desired cell type. For example, the plasmid of FIG. 3A can be used for the creation of a promoter library or a vector can be created using a commercially available vector and primers to create a three or more fragment annealing and ligation reaction as provided above. Preferably, the inclusion of a dominant negative selectable marker on the vector (e.g., the neomycin phosphotransferase gene, conferring G418 drug resistance) can be used to reduce the likelihood that cells without the vector are being maintained in culture.

Multiple allelic copies of DNA (cell derived or cDNA) can be amplified in separate reactions as a set of potential inserts with each set having its own unique overlap sequence following digestion with a restriction endonuclease, according to this invention. The fragments can then be ligated into an existing vector or in a single reaction of three or more fragments to form a combinatorial collection of potential alleles. For example, if six adjacent regions are amplified from five separate alleles, the number of combinations would be 5⁶, or 15,625 potential combinations. The combinations can then be grown en masse, and selected in vitro or in vivo. A variety of screening strategies can be used in this invention and those of ordinary skill in the art will appreciate that the type of screen will match the type of library being generation. Therefore, for the promoter library, introducing members of the library into particular cell types to assess for expression in one or more cell types versus the absence of expression in another cell type is evidence of tissue-specific or cell-specific expression. For screening purposes, the libraries of this invention function like other libraries created through other methods. A variety of screening methods for a variety of libraries have been described in the art. For example, selective screens are reviewed by Hodgson et al. (1996, RG Landes Company, supra). Reporter protein production is well known in the art as is dominant selectable marker (e.g. drug) selection and selection by fluorescence activated cell sorting, antibody affinity selection, phage display selection (such as commercially available from Amersham, Milwaukee, Wis.), and the like can be used without detracting from this invention.

In this way, it is possible to isolate multiple forms of genes, gene fragments or regulatory regions such as transcriptional promoters or packaging signals (for example, in a retro-vector system). The individual constructs may then be tested in vitro or in vivo to further characterize a particular phenotype.

In one example the method is used to create a library of complementarity determining regions (e.g., allelic variations that give rise to antibody diversity) of antibodies or from receptors, including T-cell receptors, epitopes, antigens, ligands and the like. For example, where a library of T-cell receptors is created, the introduction of a vector designed to create a functioning T-cell receptor can be introduced into T cells or T-cell progenitors and the cells can be tested for their ability to bind to a particular test ligand. The ligand-recognizing cells can then be isolated from the ligand and grown in the presence of cytokines to produce specialized T cell clones. Where a library of antibodies or antibody fragments is created, the antigen reactive portions, for example, can be recombined in a vector containing the remaining portions of an antibody molecule to generate antibodies or antibody fragments in a cell. In other examples, the methods of this invention can be used to create allelic domains of receptor families (such as the steroid receptor super-family); libraries with related regions from peptide hormones; cytochromes P450; or other protein families that have shared domains or sub-sections with similar structures. The methods of the instant invention allow the joining of allelic sub-sections in an ordered fashion. In each case, it will be necessary to design primers, and to keep track of the uniqueness of joining overlaps and the presence of internal restriction sites as described above. While these will be different in each case, here are listed some general guidelines that are incorporated into the method of the instant invention.

As discussed above, although described as it relates to promoter libraries, libraries of other nucleic acid sequences can be created using the methods of this invention. These libraries include, introns and/or exons and/or functional domains libraries, libraries of potential alleles for a particular gene sequence, and the like. These sequences can be amplified from cell DNA or RNA using the primers of this invention and incorporated into a variety of vectors. For example, one vector of this invention, VLBPGN, has a portion of LTR removed and can be used to create a variety of libraries following digestion with Bpm1.

Selected or screened products of the combinatorial library can be used for gene expression, such as the promoters of FIGS. 5 and 6. In addition, the exploitation of these sequences for the expression of a variety of genes, the LTR fragment containing the promoter can be joined to one or more functional retroviral packaging signals, internal ribosome entry sites, additional promoters, coding regions, processing sites, and the like.

Advantageously, there are almost no spatial constraints upon the joining of molecules by the method of the instant invention and other methods have not taken advantage of the combination of PCR to isolate genes or gene fragments; enzymes cleaving at a site distant from their restriction endonuclease recognition site to combine three or more fragments with precision; and, the use of unique overlapping non-palindromic termini to ensure fidelity of multi-fragment ligations. This combination permits the artisan to prepare complex gene constructions in one ligation step and does not require sequential sub-cloning into a vector or propagation in a prokaryotic host. Added to this the combination by these methods of fragment pools facilitates recombinatorial genetics.

The ability to recombine (in the correct order and direction) and screen a large number of allelic variants (whether as a simple library or as a combinatorial library), resulting in increased abundance (by amplification in the RNA, and subsequently in the DNA) is a special characterisitic of this invention. Particular advantages of this system are obtained when the methods of this invention are combined with retrovirus vector technology or other virus vector technology. For example, the combination provides a form of in vitro evolution whereby the passage of the library through virus and through cells selects functioning sequences and increases the abundance of the surviving RNA and DNA molecules.

For example, consider the consequences of screening several different promoters expressing RNA in a donor cell (i.e. a cell producing virus particles), but at differing levels of RNA abundance. In the following example, the least abundant RNA species is expressed at 0.1 copy of RNA per cell, while six others are expressed at 1 copy, 10 copies, 100 copies 1,000 copies, or 10,000 copies, or 100,000 copies/cell, respectively. After a single passage, the DNA copy number in the recipient cells now reflects the approximate RNA copy number in the donor cells. These numbers are further amplified in the relative abundance of RNA species produced in the recipient cells. Disallowing for factors such as position effects, transcription factor depletion, etc., (which may be considerable), the same relative ratios of expression would be expected. Taking into consideration position effects, the disparity between abundance caused by changing insertion loci should average out. The most abundant RNA species after two passages is then many orders of magnitude more abundant than the least abundant.

TABLE 2 Enhancement of DNA and RNA copy number as a result of different RNA expression levels, after retroviral passage. P = (no. of passages). Numbers are interpreted as relative ratios within a column. RNA DNA copy RNA DNA copy RNA abundance no. abun. no. abun. Species P = 0 P = 1 P = 1 P = 2 P = 2 A 0.1 copy/cell 0.1 0.01 0.01 0.001 B 1 1 1 1 1 C 10 10 100 100 1,000 D 100 100 10,000 10,000 10⁶ E 1,000 1,000 10⁶ 10⁶ 10⁹ F 10,000 10,000 10⁸ 10⁸ 10¹² G 100,000 100,000 10¹⁰ 10¹⁰ 10¹⁵

The present invention is able to efficiently create a library of RNA or DNA sequences whether or not they are in low abundance. The kinetics of screening for RNA abundance of a promoter can be appreciated best in the following discussion. For the purposes of this discussion, position effects have been ignored. An equation describing the kinetics of screening for RNA abundancy is:

R _(relχ) =Aχ/ΣA _(a∞)  (1)

The above equation (1) can be stated in plain English: The relative abundance of an RNA species χ ([R_(relχ)]within a population of RNA molecules expressed in a single cell or within a population of cells) is equal to the RNA copy number of RNA species χ (A_(χ)) divided by the sum of the RNA copies of all RNA species present, including χ.

The relative abundance number of any given species changes as the number of passages change, according to the following approximation:

R _(χpy) =D _(χp0) R ^(p+1)  (2)

In the simplest of terms, equation two (2) can be expressed as: The abundance of RNA species χ after Y passages (R_(χpy)) is equal to the initial abundance of the DNA for species χ at passage=0 (D_(χp0)), multiplied by the RNA abundance/DNA copy, raised to the power of the number of passages plus one. Thus, a typical RNA species that starts out as a single copy of DNA, after zero passages (i.e., in the donor cell) expresses 10 copies of RNA/cell. After one passage it is amplified at the DNA level to a relative ten copies (the same as the RNA abundance at P=0), and at the RNA level to 100 copies (10 copies per DNA copy). The reason for the amplification is that viral packaging and passage is based upon the number of RNA copies present in the donor cell. These calculations can be used to arrive at approximate abundance determinations for any given passage. The actual results of any given experiment, of course, will be biological rather than physical or mathematical. This means that other variables such as RNA efficiency of transmission and longevity, availability of transcription factors, experimental variation, etc. also come into play. The underlying purpose of the approximating equations, however, is to illustrate that RNA is amplified in DNA in proportion to the abundance of the template (RNA) within the cell.

The abundance of mRNA in cells can vary continuously from less than a copy per cell to nearly 100,000 copies/cell in actively transcribing, highly-specialized cells such as reticulocytes, the chicken oviduct, the silk mote silk gland, etc. Therefore, the spectrum of RNA abundance from 0-10⁵/cell is within the biological window of interest. For most practical purposes, such as biotechnological expression of genes in specific cells, only the higher end of this abundance range is desired. Therefore, using a viral selection system, as disclosed in this invention, it may be possible to disregard those species with less than a threshold level, such as <0.1 copies per cell. The selection through virus will lead to the recovery of the more abundant species. Furthermore, because the vector is likely to be the only considered sequence, it may be considered as a proportion of the whole of RNAs expressed in the target cell. The situation is more complex when a large number of permutations and combinations is generated, for example by self-assembling thousands or millions of fragments in a predetermined order using the self-assembly technique of the instant invention. Consider the assembly of allelic variants of four promoter subregions: distal enhancer, proximal enhancer, distal promoter and proximal promoter. If 100 varieties of each of the four groups were amplified and combined using the instant process along with a single vector, 10⁸ resultant combinations could occur. However, a sufficient number of molecules to start out a combinatorial screening program might be a million. The problem can be simplified by considering these in groups as follows:

TABLE 3 Grouped abundance of RNA molecules derived from combinations. No. of species RNA Total No. RNA RNA at RNA at RNA at in group: abundance: molec. at P = 0: P = 1 P = 2 P = 3 9 × 10⁵ 1 9 × 10⁵ 9 × 10⁵ 9 × 10⁵ 9 × 10⁵ 2 × 10⁵ 10 2 × 10⁶ 2 × 10⁷ 2 × 10⁸ 2 × 10⁹ 2 × 10⁴ 1,00 2 × 10⁶ 2 × 10⁸ 2 × 10¹⁰ 2 × 10¹² 1 × 10³ 1000 1 × 10⁶ 1 × 10⁹ 2 × 10¹² 2 × 10¹⁵ 1 × 10¹ 10,000 1 × 10⁵ 1 × 10⁹ 1 × 10¹³ 1 × 10¹⁷ 1 100,000 1 × 10⁵ 1 × 10¹⁰ 1 × 10¹⁵ 1 × 10²⁰ Sum Total: 6.6 × 10⁶ 1.11 × 10¹⁰ 1.01 × 10¹⁵ 1 × 10²⁰

Thus, it follows that in the example population (Table 3) of over a million constructs (equally represented in the DNA), a single construct expressing 105 copies of RNA per DNA copy will increase to approximately 99% of the total expressed RNA sequences in two passages. Using similar procedures in combination with drug and/or hormonal stimulation, and after consideration of the possible transcription factor binding sites within the sequence family (FIGS. 5 & 6), it is within the intended scope of the invention to select for hormonal or pharmacological controls of transcription such as have been described herein. The factors contributing to the outcome are not only the input constructs, but recombinants and mutants as well. These secondary contributors to molecular diversity will be enhanced if multiple rounds of infections are allowed to occur, as oftentimes the difference between a particular transcription factor being able to bind (or not) may depend upon a single base change. Because viral infection is progressive and competitive, molecular evolution can be used to generate gene constructs de novo in the tissue culture dish in short time periods. Advantageously, the use primers to generate amplified fragments with uniquely complementary cohesive ends (i.e., that the ends will preferably only hybridize with the intended 5′ and 3′ fragments) to ligate three or more fragments as taught in this invention improves the potential for obtaining a diverse library.

Although the examples particularly point out a transcriptional promoter as the product of the process, the skilled artisan can appreciate that a particular selection technique can be applied to other cis- and trans-acting genetic sequences as well. Although a virus is used to propagate the selective advantage of a preferred embodiment, it can also be appreciated that any selective screen, such as drug selection, cell survival, phenotypic selection, cell sorting, antibody selection, and the like (see Ausubel et al., supra) could be substituted without changing the intended scope of the invention. Likewise, transfection or cell fusion could be used in place of viral infection. Furthermore, substitution of different viruses, retrotransposons, or functional groups are likewise within the intended scope of the invention. The described embodiments are to be considered only as illustrative and not restrictive, and the scope of the invention is indicated by the claims rather than by the narrative description. All references and publications, cited herein, are incorporated by reference into this disclosure.

Like the embodiments detailed above, the method of library production is also conducive to assembly and transfer of genetic material directly into eukaryotic cells, saving the step of propagation in bacteria that is standard in bacteria. An advantage of direct transfer of the libraries of this invention to eukaryotic cells, including the exemplary retroviral vector producer cells, is that certain essential cis-acting structural features will be under positive selection (i.e., if they are not present, the molecule will be lost due to its non-functionality). As discussed above, it is often advantageous to eliminate bacterial and plasmid DNA sequences, endotoxin, and other bacterial contaminants by introducing the constructs directly into eukaryotic cells.

In addition to providing a method for constructing complex DNA molecules efficiently (as in the examples of three piece and six piece constructs), the methods of this invention permit the assembly of constructs that are larger than those conventionally propagated in E. coli. Examples of these types of vectors include adenovirus vectors, herpes simplex vectors and artificial minichromosomes. In order to insert genes into such vectors that are too large for conventional molecular cloning procedures, in the past it was often necessary to resort to in vivo recombination, wherein the genes of interest are cloned into a suitable vector and the flanking homologous regions are used to target the foreign genes to a homologous site within the larger viral or minichromosome vector. However, the methods of this invention permit PCR fragments of any size (up to the limits of PCR capability, 20-30 kb per fragment) to be joined together. Thus, it is feasible to precisely construct adenovirus vectors by amplifying larger sequences, and combining them by ligation. For example, several sections of adenovirus (5-10 kb each) can be ligated using the methods of this invention, up to for example, about 37 kb, and then transformed directly into human cells. Only the correctly recombined vectors are capable of replicating. Hence, the DNA is autoselecting. A similar procedure is used for, generating herpes virus vectors, which are approximately 150 kb. The precision of the methods of this invention permit non-essential viral genes to be more easily eliminated from the construct. After transfection into appropriate cells, the DNA replicates and virus particles are formed.

Some special considerations apply to larger vectors, however. First, it is desirable to use enzymes that do not cut within the large DNA fragments. To prevent excessive fragmentation of the DNA by internal sites, it is desirable to use enzymes that cut rarely or infrequently, such as CpG-containing enzymes recognizing six bases, or enzymes such as Sap1, recognizing seven bases and digesting a three bp overhang (thus permitting up to 32 fragments to be joined in order). It is also desirable to avoid shearing the DNA once large segments have been joined by ligation. One method of avoiding shear is to add the transfection agent, such as Superfect™ reagent (dendrimers, Qiagen) or Lipofectamine™ (liposomes, Life Technologies, Gaithersburg, Md.) directly to the ligation reaction, and then add the cells to be transfected to the mixture. This, or a similar method avoids the need to physically move the ligated DNA, and thus prevents shearing. Another method is to add a DNA condensing reagent (dendrimers, polycations [such as polyethyleneamine] histones or liposomes) directly to the DNA ligation reaction, and then move the DNA by pipette after it has condensed (thus reducing shearing of the DNA). Once inside the cell, viral DNA can replicate (as in the examples of partially replication-competent adenovirus and herpes simplex virus vectors).

Artificial minichromosomes have been under development for years. True artificial chromosomes require a centromere, at least one origin of DNA replication, and in the case of linear molecules, telomeric repeats at the chromosomal termini. In addition, to be very effective it is desirable to have a selectable marker gene, one or more therapeutic genes, and/or reporter genes.

In reality, the use of minichromosomes has been delayed by the inability to effectively manipulate the larger DNA molecules in vitro. Yeast and bacterial artificial chromosomes have been used with little success in mammals, and the addition of telomeres to the ends of linear chromosomes is also a special problem, as there is no prokaryotic host that can tolerate large linear DNA. The methods of this invention offers the opportunity to assemble human or mammalian minichromosomes in vitro, by using large segments (10-30 kb) of synthetic, gene-amplified DNA as ligation starting materials. For example, up to 32 Sap1 fragments (up to 30 kb each, containing the essential cis- and trans-acting sequences), or 512 shorter Hga1 fragments can be combined using these methods. As with the other examples, several enzymes suitable for this invention (e.g., such as class IIS enzymes) can be combined (possibly with different termini lengths) to simplify the task. The methods of this invention also facilitate construction of telomeric repeats, because the constructs of this invention do not need to be circular. Thus, the methods of this invention can be used to make telomeres of any length, by adding additional segments onto the ends of molecules. One way to do this is using self assembling genes that employ a repeating overhang sequence (self-complementary molecule, such as AG-3′ at one end, and CT-3′ at the other end), permitting the telomeres to be lengthened to the extent desired by adding the required molar excess of the telomeric repeat-containing fragment. This technique gives the investigator some control over the relative length of the telomeres, although the self-complementarity indicates that many repeats will be lost due to self-ligation. This can be alleviated by using higher starting concentrations of DNA to favor inter-molecular ligations over intra-molecular ligations (e.g., >20 μg/ml starting concentration of DNA).

A two fold molar excess of telomeric fragments gives approximately twice the average length of telomere as a strictly 1:1 molar ratio of all fragments. By using a higher molar ratio of shorter telomeric repeats it is possible to give greater uniformity to the overall length of the molecules, which will vary from one terminus to the other. Thus, in addition to providing a way to build large molecules with precision, the methods of this invention provides for a way to control the telomere length (or potential life-span) of the artificial chromosome. To prevent damage during handling, the minichromosome DNA can be condensed with polycations, adenovirus particles, dendrimers, histones, or liposomes prior to transfection, similar to larger viral vectors.

The methods of this invention can be used to create recombinant virus. One example of this is an adenovirus vector self-assembling gene system. This system can include three parts: 1) vector: 2) helper virus; and 3) helper cells. The vector part is a self-assembling fragment set of at least three fragments comprising the essential cis-acting sequences (left and right inverted terminal repeats, which are the 103 bp at both ends of the genome that are required for replication [ITRs] and packaging sequences [Y, base pairs 194-358) and central ‘baggage’ area, comprising one or more self-assembling fragments including therapeutic genes, marker genes, and reporter genes. The baggage area is thus flanked by the cis-acting sequences in the vector. Because the synthetic oligonucleotide sequences comprising the 5′ and 3′ termini of the helper virus are not phosphorylated, they will not ligate together creating multimers. Thus, the Ad5 vector region will assemble only into monomers. The helper virus part comprises all Ad5 trans-acting genes except for the E1A and E1B genes. The helper virus part has no cis-acting sequences, and it is amplified in several sections. In this preferred embodiment, the virus is amplified using primers that exclude the ITRs, packaging region and E1A&B genes. The helper virus is digested by Sap1 digestion, creating seven uniquely terminated fragments comprising the trans-acting viral genome, with dephosphorylated, blunt 5′ and 3′ ends on the terminating fragments. The primers are designed so as to amplify the internal virus sequences without changing them, except for the 5′ and 3′ ends of the virus. The PCR-amplified fragments are digested with Sap1 and are religated in their natural order after gel isolation and Qiagen column purification. The 5′ end of the helper virus genome starts at 3.2 kb (in the E1A gene) so as not to overlap the vector sequences, which could otherwise cause replication competent adenovirus (RCA). Because the 5′ and 3′ ends of the helper virus do not contain Sap1 sites, they remain intact after digestion with Sap1. Because the synthetic oligonucleotide sequences comprising the 5′ and 3′ termini of the helper virus are not phosphorylated, they will not ligate. Thus, the Ad5 helper virus genome assembles only into preferred monomers during ligation.

In a preferred embodiment, non-essential genes are deleted from the Ad5 genome by means of the method of self-assembling genes. In another preferred embodiment, the helper virus genome is approximately 30 kb after deletion of E1A, E1B and E3 gene sequences from the helper virus, and it is amplified as a single long fragment using the eLONGase Amplification System (Life Technologies or a similar strategy for creating long PCR fragments with high fidelity). It is not of great importance that occasional PCR errors may occur, because multiple copies of the Ad5 helper virus are transfected into target cells, thus providing trans-complementation. The helper cells are preferably 293 cells, a human kidney cell line expressing E1A and E1B genes: (ATCC). The vector part and the helper virus part are combined in equimolar ratios after ligation has been performed separately on each fragment set. The Superfect protocol (Qiagen) Iis used to transfect the vector part and the helper part into the helper cells. The helper cells lyse, releasing high-titer adenovirus particles that are capable of infecting a variety of human cells. The resulting defective virus is incapable of forming RCA, and it transmits up to 34 kb of foreign genes in the baggage area. Unlike conventional Ad5 vectors that require separate constructs for E. coli propagation of insert genes, and recombination in vivo, the present vectors are relatively easy to make and provide a precise, safe alternative to first generation and second generation adenovirus vectors.

Exemplary methods for producing self-assembling vectors and genes are provided below. Further, the Examples provide methods for producing libraries of nucleic acid sequences using the methods of this invention. A number of nucleic acid sequences identified using the methods of this invention are described. The examples provided below are exemplary and not limiting. All references and publications provided herein are incorporated by reference into this disclosure.

EXAMPLE 1 Three-Piece Gene Self-Assembly with 100% Efficiency

Using 6 primers (SEQ ID NOS:24 and 63-67), three PCR fragments were amplified from templates VLMG (SEQ ID NO:22) and VLBPGN (SEQ ID NO: 1). PCR reactions were carried out using the hot start technique, according to the manufacturer's instructions (Perkin Elmer) using Pfu DNA polymerase (Stratagene). To amplify specific portions of the above templates, each primer contained a class IIS enzyme site capable of digesting a unique overhanging end that was complementary to only one other terminus in the subsequent ligation. The class IIS enzymes used were Bpm1 and Eco 57I (the latter was used to copy a fragment that contained an internal Bpm1 site). The reactions were carried out as follows: 1) the lower reaction was assembled according to the protocol for PCR Gems (Perkin Elmer); 2) the lower reaction was heated to 80° C., 5 min, then cooled to 4° C. for 5 min; 3) the upper reaction was prepared according to PCR Gems protocol and was added to the lower reaction (separated by cooled wax). The primer concentration was 0.3 μM (final). The dNTP concentration was 200 μM (final). 5 Units of Pfu polymerase was used. All fragments were amplified using the following conditions: 96° C.; 45 sec; (then followed by 30 cycles of the following) 96° C. 45 sec, 52° C. 45 sec, 72° C., 6 min; then followed by a single incubation at 72° C. for 10 min; then hold at 4° C. All fragments were successfully amplified. The PCR fragments were purified using the Qiaquick PCR purification protocol (Qiagen). The fragments were digested with an excess of the appropriate restriction enzyme (Bpm1 or Eco57I). The digested fragments were run on a 1% agarose gel and were excised using minimal irradiation from a hand-held 365 nm ultraviolet light. The fragments were purified using the Qiagen Qiaquick Gel Purification Protocol. The fragments were ligated at an equimolar ratio at a concentration of >20 μg/ml with T4 DNA ligase (Boehringer Mannheim) overnight at 4° C. Competent E. coli SCS110 cells (Stratagene) were transformed with the ligated DNA. Eight colonies were characterized by restriction enzyme analysis, and all eight contained the correct order and orientation of the three fragments. The experiment was repeated independently by another investigator, and the same result was obtained (8/8=100%). Thus, the procedure resulted in a high percentage of correctly assembled vectors.

This three-piece vector was VLΔBP. The deletion extended from the distal enhancer region to the TATA box near the start of transcription. The deletion region was a pair of Bpm1 sites that permitted U3 sequences to be cloned into the insert.

One validated E. coli clone of VLΔBP was transfected into retroviral helper cells. After 48 h, the vector was transduced into amphotropic helper cells. After selection for two weeks with the drug G418, drug resistant colonies were grown up in a mass culture and the vector was transduced from the amphotropic helper cells into a human HT1080 cell line (ATCC, Rockville, Md.). Surprisingly, even with a large deletion in the LTR promoter, the basal TATA box-containing VLΔBP was transmitted as a retrovector and was permanently inserted into the human cell line, thus establishing the validity of the self-assembly technique for the construction of functional eukaryotic vectors.

EXAMPLE 2 Production of a Six Piece Self-Assembling Expression Vector

Due to the high efficiency of the gene self assembly process for the three piece assembly, a complex vector containing six fragments was constructed. The results here were extended to determine whether such a self-assembled vector would also have biological activity in human cells without being cloned and grown in a prokaryotic cell.

Six fragments were individually constructed by PCR using three different templates and twelve primers (as illustrated in FIG. 8). The primers used three different class IIS enzymes. The enzymes were chosen so as to give 2 base pair, 3′-overhanging ends. Three enzymes were used in order to avoid the use of enzymes that had additional sites internal to the fragments being amplified. Thus, Bpm1 was used unless there was an internal Bpm1 site. If such a site existed, Eco57I was used. If there was also an internal Eco57I site, then BsrD1 was used. However, it is alternatively possible to use an enzyme such as Eam11041, where the Eam11041 sites in the primers are unmethylated (therefore susceptible to digestion by the enzyme), and wherein the ^(m5)dCTP analog of dCTP is used in the PCR reaction, methylating all internal sites (and protecting them from digestion by Eam11041), as suggested by Padgett and Sorge, Gene, 168(1):31-35, 1996, and incorporated herein by reference.

Using 12 primers, 6 fragments were amplified from 3 templates: pBK-CMV (SEQ ID NO:26), pVLMB (SEQ ID NO:23) and pVLOVhGH-900 (SEQ ID NO:21). Fragment 1 was amplified from pBK-CMV using primers 1 and 2 (SEQ ID NOS:31 and 32). Fragment 2 was amplified from pVLMB using primers 3 and 4 (SEQ ID NOS:33 and 34). Fragment 3 was amplified from pVLOVhGH-900 using primers 5 and 6 (SEQ ID NOS:35 and 36). Fragment 4 was amplified from pVLMB using primers 7 and 8 (SEQ ID NOS:37 and 38). Fragment 5 was amplified from pVLMB using primers 9 and 10 (SEQ ID NOS:39 and 40). Fragment 6 was amplified from pVLMB using primers 11 and 12 (SEQ ID NOS:41 and 42). PCR reactions were carried out using the hot start technique, according to the manufacturer's instructions (Perkin Elmer Ampliwax PCR GEMS 100). The lower reaction was heated to 80° C. for 5 min, then cooled to 20° C. for 5 min. The upper reaction was prepared according to PCR gems protocol and was added to the lower reaction (separated by cooled wax). The primer concentration was 0.3 micromolar (final). The dNTP concentration was 200 μM (final). 5 U of Pfu polymerase (Stratagene) was used per reaction. 100 ng of template was used for each reaction 14 rounds of PCR amplification were used to reduce mutagenesis of the templates. The PCR cycling protocol was 96° C. 45 sec; then two cycles of (96° C. 45 sec, 52° C. 45 sec, 72° C. 6 min); then 12 cycles of (96° C. 45 sec, 58° C. 45 sec, 72° C. 6 min) followed by a 72° C. soak for 10 min, then to 4° C. hold.

The six PCR fragments were designed to self-assemble into a retro-vector after digestion with the correct class IIS restriction enzyme (FIG. 8). After transfection into retroviral helper cells, the vector DNA is transcribed as RNA by means of the cytomegalovirus immediate early promoter (fragment 1). This promoter replaces the retroviral or VL30 LTR in this vector. The RNA transcript region begins with the R and U5 regions of the Moloney murine leukemia virus (MoMLV) LTR, the viral packaging signals (Ψ) region of MoMLV, the packaging enhance (Ψ+) region of mouse VL30 and the IRES region of EMCV fragment 2. Fragment 3 consists of the human growth hormone (hGH) cDNA sequence. Fragment 4 consists of the SV40 virus early region promoter driving expression of the neomycin phosphotransferase (neo) gene. Fragment five consists of the (+)-strand primer binding site of the MoMLV LTR, the U3 region of the MoMLV LTR, the repeat (or R) region, and a portion of the U5 region Fragment 6 consists of the PBR322 plasmid origin of replication.

Fragment 1: CMV early region promoter Template: pBK-CMV plasmid DNA (Stratagene, LaJolla, CA) Bpml (SEQ ID NO:26) PCR primer 1 (SEQ ID NO:31) GACTAACCTTGATTCCACTGGAGCCGTATTACCGCCATGCATTAGTTATTAATAG PCR primer 2 (SEQ ID NO:32) GACTAACCTTGATTCCACTGGAGTAATTGCGGCTAGCGGATCTGACG Fragment 2: R-U5-Psi-Psi(+)-IRES Bpml Template: pVLMB plasmid DNA (SEQ ID NO:23) PCR primer 3: SEQ ID NO:33 GACTAACCTTGATTCCACTGGAGACACTTGACCTCTACCGCGCCAGTCCTCCGAT TGACTGAGTCG PCR primer 4: SEQ ID NO:34 GACTAACCTTGATTCCACTGGAGGGATCCGCGCCCATGATTATTATCG Fragment 3: human growth hormone (hGH) Bsr DI Template: pVLCNOVhGH plasmid DNA (SEQ ID NO:21) PCR primer 5: SEQ ID NO:35 GACTAACCTTGATTCCAGCAATGTCGGTTAGCTTGTTTCTTTACTGTTTGTC PCR primer 6: SEQ ID NO:36 GACTAACCTTGATTCCAGCAATGTTAGGACAAGGCTGGTGGGCACTGG Fragment 4: SV40 early promoter-neomycin phosphotransferase Template: VLMB plasmid (SEQ ID NQ:23) PCR primer 7: SEQ ID NO:37 GACTAACCTTGATTCCACTGGAGGGTCGACCCTGTGGAATGTGTGTCAG PCR primer 8: SEQ ID NO:38 GACTAACCTTGATTCCACTGGAGAATCTCGTGATGGCAGGTTGGGCGT Fragment 5: MLV(+)PBS-U3-R-U5 Template: VLMB plasmid (SEQ ID NO:23) PCR primer 9: SEQ ID NO:39 GACTAACCTTGATTCCACTGAAGAGATTTTATTTAGTCTCCAGAAAAAGGGGGG PCR primer 10: SEQ ID NO:40 GACTAACCTTGATTCCACTGAAGCCCCCAAATGAAAGACCCCCGCTGACG Fragment 6: PBR322 origin of replication Template: VLMB plasmid (SEQ ID NO:23) PCR primer 11: SEQ ID NO:41 GACTAACCTTGATTCCACTGGAGCCGGGACGGAATTCGTAATCTGCTGC PCR primer 12: SEQ ID NO:42 GACTAACCTTGATTCCACTGGAGTTCTCGAGGCGGCGCATCTCGGCG

Procedure: The twelve primers were prepared by the following procedure: 1) oligonucleotides were synthesized with trityls off. After deprotection and lyophilization, the samples were resuspended in 5 microliters deionized formamide and loaded onto a polyacrylamide gel (12% polyacrylamide, 250V). The samples were excised under short wave UV irradiation and eluted overnight in 600 microliters of sample elution buffer (0.5 M ammonium acetate, 10 mM Mg acetate, 1 mM EDTA, 0.1% SDS). The contents were loaded onto a BioRad Chromatography column (Cat. # 732-6008) and centrifuged into an Eppendorf tube at low speed (2000 RPM, 5 min). After washing the column with 500 microliters TE buffer (10 mM Tris, 1 mM EDTA), pH 8.0 and recentrifugation (2000 RPM, 5 min), the pooled eluate was ethanol precipitated, washed with 100% ethanol, resuspended in TE buffer and quantitated by spectrophotometry of a small sample, which was then discarded.

Fragments were cleaned using the Qiaquick PCR cleanup procedure. The fragments were digested with their respective class IIS restriction enzyme. The digested fragments were run on 1% agarose gels, and the fragments were excised and cleaned using the Qiaquick gel cleanup procedure. Fragments were combined in an equimolar mixture and ligated overnight at 4° C. with T4 ligase and ATP. An analytical gel was run with the ligated DNA, as well as with controls including unligated fragments and ligated fragments with a single fragment missing. As opposed to the controls, the complete ligation included bands equivalent to the full-length supercoiled monomer (refered to as GENSA 981, SEQ ID NO:29), as well as bands possibly representing multimers (up to six bands were observed).

In order to assess the efficiency of the method, eleven nanograms of DNA were transfected into SCS1 supercompetent cells. Thirteen kanamycin resistant colonies were harvested, and plasmid DNA preps indicated 10 out of thirteen that appeared to be the correct length. All ten gave the expected bands when digested with Pst1, SnaB1, and Bam HI. 1.35 μg of the ligated DNA was purified by phenol-chloroform-isoamyl alcohol extraction, followed by two extractions with chloroform-isoamyl alcohol, and was precipitated in ethanol. The DNA was washed in 70% ethanol and re-suspended in 50 μl of sterile phosphate buffered saline (for transfection). The DNA was transfected (using the Qiagen Superfect protocol) into HTam1 (amphotropic human helper cells). 24 h after transfection, the target cells were washed and fresh culture media was added. 48 h after transfection, the supernatant from the vector producer cells was filtered (0.45 μm, Nalgene) and transferred to PG13 helper cells (ATCC) and HT1080 human fibrosarcoma cells. This procedure was repeated after 72 h. 48 h after transduction, recipient cells were started on G418 drug selection (500 μg/ml). The appearance of G418 drug-resistant colonies on transduced PG13 and HT 1080 cells after 6 days of selection indicated successful transmission via retrovirus particles. The transfect HTam cells were also selected with G418. After six days of drug treatment, 45 colonies of resistant cells were counted. Thus, the six fragment gene assembly was effectively transmitted and expressed as either a DNA (transfection) vector or a retro-vector.

EXAMPLE 3 Design and Construction of Single LTR Vectors

Background: In order to manipulate the interior of the VL30 LTR sequences using a promoter rescue technique, single LTR vectors were constructed. The mouse VL30 element NVL-3 was used as the starting material as it is constitutively and abundantly expressed in most mouse tissues. Single LTR vectors are circular and behave as if they contained two LTRs. Thus, in these vectors RNA transcription begins at the start of the R region (see FIG. 3B), and continues through the polyadenylation site after completing the second round of transcription of the R sequences (FIG. 3A). In previous studies, these vectors were expressed transiently in vector producer cells and the DNA did not integrate into cell DNA as a standard two LTR vector. Therefore, the vectors were usually passed to a second complementation helper cell line via retroviral transduction of the vector RNA transcribed in the first helper cell. This process resulted in the vector regenerating a correct (two LTR) structure upon integration into the recipient cell DNA.

Experimental method: The plasmid pNVL-3 (SEQ ID NO:25, kindly provided by Dr. J. Nortonm Manchester, UK), containing a complete copy of the NVL-3 (mouse VL30) genome (Adams et al., Mol. Cell. Biol., 8:2989-2998, 1988), was digested with Xho1 (which cuts in the LTRs), releasing the 4.27 kb VL30 genome with one copy of the LTR. This fragment was circularized using T4 DNA ligase and ATP. The circular DNA was linearized by digestion with SnaB1, 187 bp from the 3′-LTR. A 2.3 kb fragment containing the SV40 virus early region promoter and the aminoglycoside phosphotransferase (neo) gene, together with the PBR322 plasmid origin of replication, was excised from the BAG retrovirus vector (Price et al., Proc. Natl. Acad. Sci. 84:156-160, 1987, kindly provided by C. Cepko, Cambridge, Mass.). BAG is also obtainable in a retrovirus helper cell line from American Type Culture Collection (ATCC), Rockville. Md. by digestion with Xho1 and BamHI. This fragment was blunted with T4 DNA polymerase and dephosphorylated with calf intestinal alkaline phosphatase (CIP). The fragment was then ligated to the linearized SnaBI fragment of NVL-3 . The resulting plasmid (containing a circularly permuted NVL-3 genome with the SV-neo-ori region) was designated VLSNO2 (SEQ ID NO:30).

In order to facilitate the switching of LTR sequences by means of the class IIS enzyme Bpm1, VLSNO2 was digested with Bpm1 (six sites). The region containing four Bpm1 sites was removed and replaced with a 19 bp linker (SEQ ID NOS: 1 and 52, see below), 921 bp beyond the LTR. The linker contained Sna BI, Cla1 and Bam HI cloning sites.

Linker (top strand): 5′-TACGTATCGATGGATCCGA-3′ (SEQ ID NO:51)

Linker (bottom strand): 5′-GGATCCATCGATACGTAAG-3′ (SEQ ID NO:52)

The remaining two of the Bpm1 stes had complementary ends, which permitted their ligation and resulted in eradication of all Bpm1 sites within the resulting vector VLSNO3 (SEQ ID NO:20).

In order to facilitate reporter/therapeutic gene function, a 3.7 kb fragment containing the internal ribosome entry site (IRES) from encephalocyocarditis virus, together with the β-galactosidase reporter gene, was excised from the plasmid pVLSAIBAG (kindly provided by Mr. James Grunkemeyer, Omaha, Nebr.) by means of a partial digestion of the plasmid with Bam HI. This region was inserted into the Bam HI site of VLSNO3, resulting in the vector VLSNOSIB (SEQ ID NO:14).

A second reporter construct, pVLSNOG (5774 bp, SEQ ID NO:19) contained the green fluorescent protein (GFP, Clontech, Palo Alto, Calif.) gene was constructed by inserting a Bgl2-Bcl1 fragment (800 bp) from plasmid pGFP-N1. This sequence, containing the GFP gene, was treated with mung bean exonuclease and inserted into the unique Sna B1 site of pVLSNO3.

In order to enhance GFP fluorescence from the reporter plasmid pVLSNOG, the serine-65 codon in the GFP gene was mutated into threonine by a site-directed mutagenesis procedure with the Transformert™ Site-Directed Mutagenesis kit from Clontech. A Bpm1 site in the GFP gene (threonine-9) was mutated at the same time without changing the amino acid (ACT to ACA). The resulting plasmid was pVLSNOGM (SEQ ID NO:18).

An Nco1-Xho1 fragment (585 bp) from plasmid pG1IL2EN (kindly provided by Dr. Steven Rosenberg, Bethesda, Md.), containing the internal ribosome entry site (IRES) from encephalomyocarditis virus (EMCV) was inserted into the Apa1 site upstream of the GFP gene in pVLSNOGM, resulting in pVLSNOGMI (SEQ ID NO:17). Both insert and plasmid fragments were blunted with mung bean exonuclease. One variant version of pVLSNOGMI with an IRES tandem dimer was also constructed and designated pVLSNOGMI2 (SEQ ID NO:16).

Oligonucleotides (SEQ ID NO:53 and 54) containing a splice acceptor (SA) of AKV virus (in bold) was inserted into pVLSNOGMI at the unique Sac 2 site just before the IRES, resulting in pVLSNOGMIS (SEQ ID NO:15).

Oligo: (SEQ ID NO:53) 5′-GGCCGCTAACTAATAGCCCATTCTCCAAGGTACGTAGC-3′

3′-CGCCGGCGATTGATTATCGGGTAAGAGGTTCCATGCAT-5′ (SEQ ID NO:54, bottom Oligo)

Recovery of LTR Promoter Sequences from Mouse CD4+ T-helper Cells

In order to facilitate the recovery of VL30 promoter sequences expressed in mouse T-helper cells, a mouse CD4+ T-helper cell cDNA library (Stratagene, San Diego, Calif., Catalog #937311) was screened by plaque hybridization. Approximately 2×10⁴ bacteriophage λ-ZAP clones were plated on a lawn of E. coli cells according to the manufacturer's instructions. Two nylon filters were sequentially layered onto the lawn of E. coli cells and bacteriophage. The filters were hybridized to a ³²P-labelled (Prime-It RmT Random Primer Labeling Kit, Stratagene), 4.2 kb internal Xho1 fragment of NVL-3 (containing the NVL-3 genome). 55 plaques (of approximately 0.3% of the total phage) reacted positively on both filters. 18 VL30 cDNA sequences were cloned from the plate, which was used to identify U3 promoters that at actively expressed in the RNA of mouse T-cells. Five of the 18 clones contained intact U3 sequences, representing four of one molecular species, named TH1 (SEQ ID NO: 2) and one of another species, named TH2 (SEQ ID NO: 3) also provided in FIG. 5. TH1 contained approximately 120 bp more DNA than did TH2. Because TH1 was more abundant (4 out of 5 clones), the additional sequences in the enhancer region were implicated to be a possible reason for the stronger expression in mouse T cells. Examination of the known and putative transcription factor binding sites in the VL30 LTR (Hodgson, 1996, chapter 4, FIG. 4.2 supra) revealed several interesting features of TH1 and TH2. First, the extra sequences of TH1 that were missing in TH2 included an extra copy of the enhancer repeat region as well as a potential retinoid (RAR/RXR) binding site. Several transcription factor binding sites in the enhancer repeat region that differed between the two elements included: a cyclic 3′-5′ AMP response element (VLCRE, a potential CREB/jun binding site), a serur response element (SRE), and a potential NF1/IL6 binding site (although there were additional sites for these factors in other enhancer repeats). These factors could possibly explain why VLTH1 appeared to be expressed at higher levels, both in the source cells and into transduced cells. Together, the VL30 sequences represented 0.3% of the mRNA expressed in the T cells, and TH1 appeared to be most abundant VL30.

Sequencing Primers

(SK, SEQ ID NO:49) 5′-CGCTCTAGAACTAGTGGATC (20 mers, Tm 60° C.).

(T7, SEQ ID NO:50) 5′-GTAATACGACTCACTATAGGG (21 mers, Tm 60° C.).

Seamless Rescue of T Cell Promoters Using Class IIS Restriction Enzymes

Two sets of primers containing offset Bpm1 restriction sites were designed and synthesized. One set was for amplification of the plasmid sequences, and another was for the amplification of the inserts.

Insert Primers: (Bpm1 Site Bold)

ITA (43 mer, Tm: 67.2° C., SEQ ID NO:45) CGATCCACTGGAGCTCGGAGCCCACCCCCTCCCATCTAGAGGT

ITB (43 mers, Tm: 66.3° C., SEQ ID NO:46) CGTCCTCCTGGAGAGCACAGGGTAGAGGAGTCTCGACGGTCAG

Vector Primers: (Bpm1 Site Bold)

VLA (43 mers, Tm: 68.2° C., SEQ ID NO:47) CGCAACCCTGGAGACCTCTAGATGGGAGGGGGTGGGCTCCGAG

VLB (43 mers, Tm: 66.3° C., SEQ ID NO:48) GCAGGACCTGGAGCTGACCGTCGAGACTCCTCTACCCTGTGCT

To amplify vector sequences more efficiently, vector templates were shortened by deleting marker genes from vectors. pVLSNOSIB (SEQ ID NO:14) was cut with Kpn 1 and a 4201 bp fragment containing β-gal gene was removed. The remaining vector has 3923 bp.

The U3-promoter inserts (357 bp for TH1 and 240 bp for TH2) were PCR-amplified from TH1 and TH2 promoters with primers ITA and ITB. The vector cassettes (˜4.2 kb for pVLSNOSIB and ˜3.7 kb for pVLSNOGMIS) were PCR-amplified from the shortened vector templates using primers VLA and VLB, (supra). The PCR-amplification was done with high-fidelity Pfu DNA polymerase from Stratagene (La Jolla, Calif.). The amplified products were gel-purified (1% agarose gel). The inserts were then cut with Bpm 1 to produce complementary ends. The vector cassette products were phosphorylated with PNK, then circularized with T4 ligase, and transformed into SCS 110 cells. Recovered plasmids were then digested with Bpm 1 and treated with CIP to produce complementary ends. Bpm 1 treated inserts and vector cassettes were ligated, and T-cell tissue-specific VL 30 vectors VLTH1 and VLTH2 were produced. The marker β-gal gene and GFP gene were put back into those vectors at the original unique sites Kpn 1 and Sal 1 respectively.

Transmission and Expression of Single LTR Vectors and T Cell U3 Sequences

Vector DNA constructs were transfected into GP+E86 retroviral helper cells (Markowitz et al, 1988, supra) using the Lipofectamine protocol (Life Technologies, Gaithersburg, Md.). The culture media from these cells (supernatant), containing defective transducing particles (72 h post-transfection), was transmitted to PA317 Miller, U.S. Pat. No. 4,861,719 amphotropic helper cells, using ectamine to enhance transduction efficiency (Hodgson et al., 1996. Synthetic Retrotransposon Vectors and Gene Targetings pp. 2-14, in: Felgner et al., eds. Artificial Self-Assembling Systems for Gene Delivery. American Chemical Soc. Books, Washington, D.C.). A similar procedure was used to transmit VLTH1 and VLTH2 to the PG13 helper cell line (Miller et al., 1991. J. Virol. 65:2220-2224). 24 h post-transfection, the recipient cells were selected with the drug G418 (500 μg/ml, 2 weeks) to enrich for stably transduced cell populations.

All of the single LTR vectors, including VLTH1 and VLTH2 were transmitted by this method, indicating that single LTR vectors can be used for promoter switching and yet revert to dual LTR vectors after a single passage. Vectors VLSNO2, VLSNO3, and VLSNOSIB were then titered on NIH 3T3 cells (using the PA317 vector producer cell lines). VLTH1 and VLTH2 vectors were titered on human HT1080 cells (PG13 cell lines). Surprisingly, all of the single LTR vectors were transmitted effectively. However the titers of stably transduced TH1 and TH2 cell lines were 5.5×10²-1.1×10³ TU/ml, compared to 0.4-3.0×10⁴ TU/ml for the VLSNO2, VLSNO3 and VLSNOSIB cell lines. Thus, switching from the NVL-3 transcriptional promoter (originally isolated from NIH 3T3 fibroblast cells) to VL30 promoters derived from T helper cells, appeared to have a negative effect on RNA expression in fibroblast cells, as determined by the transmissibility of the RNA.

In order to study the usefulness of rescued promoters as DNA transfection vectors (as opposed to retro-vectors), VLSNOSIB, VLTH1 and VLTH2 were also transfected into a number of cell lines (using Lipofectamine), including NIH 3T3, PA317, GP+E86, PG13, HT1080, SW480 and HeLa (available from ATCC). RNA expression in these cell lines is shown in Table 4, wherein gene expression from the LTR promoter (as determined by β-gal staining) is normalized to VLSNOSIB (100).

TABLE 4 NIH GP + Cell line: 3T3 PA317 E86 PG13 HT1080 SW480 HeLa Vector: VLSNOSIB 100 100 100 100 100 100 100 VLTH1 39.3 18.7 0.1 21 25.5 156 156 VLTH2 28.6 7.1 5.5 11.5 46.8 82 156 Transient expression of a β-gal marker gene by three VL30 promoters: NVL-3 (VLSNOSIB), VLTH1 and VLTH2. Cells were transfected using the Lipofectamine procedure. Total blue cells were counted from each well in 6-well plates, and the number of blue cells from VLSNOSIB was normalized to 100%.

The expression of both the VLTH1 and VLTH2 promoters was significantly reduced compared to VLSNOSIB in cell lines of fibroblastic origin, whereas in SW480 colorectal cancer cells and HeLa cells, it was comparable to or better than VLSNOSIB (the NVL-3 promoter). However, VLSNOSIB was expressed poorly in the non-fibroblastic cell lines, so a direct comparison was difficult to interpret. Unfortunately, the human T cell lines (Jurkat and MOLT4 [obtained from ATCC]) were not transfected by Lipofectamine, and they were poorly transduced by VLTH1 and VLTH2 retro-vectors. In the Jurkat and MOLT4 cells transduced with VLTH1 and VLTH2, only a small percentage (1-10%) of cells that were stably transduced by the vectors stained positively for β-gal expression. However, the marker gene (neo) continued to be expressed from an internal promoter, as evidenced by drug selection.

Taken together, the results demonstrated: 1) the ability of the promoter rescue technique to seamlessly capture functional transcriptional promoters from specialized cells; 2) the ability of single LTR vectors to introduce the rescued promoters into standard transducing vectors; 3) the ability of the rescued promoters to be expressed at differing levels in several different cell types, including T cells; and 4) screening and selection established the efficacy, or lack thereof, of individual promoter sequences.

Although the general method of promoter rescue was demonstrated by the foregoing experiments, the titers obtained from the sLTR VL30 vectors may not be useful where selection systems are not available.

Additional experimentation led to the development of a chimeric packaging signal, combining the essential packaging signal from Moloney murine leukemia virus (Ψ), and the enhanced packaging signal (Ψ+) from a mouse VL30 element. A vector embodiment of this packaging system is VLMB (SEQ ID NO:23). One advantage of the chimeric packaging system was the elimination of retroviral gag gene sequences that were present in previous high-titer MLV-based vectors (viral gag sequences contribute to the generation of replication competent retrovirus outbreaks). The titers of VLMB-based vectors ranged from approximately 1×10⁵ to 4×10⁶ TU/ml.

Construction of a Cloning Vector for Promoter Rescue

Using pVLSNOGMIS as a template, and primers (SEQ ID NOS:28 and 68), a 6.4 kb plasmid fragment was PCR amplified (Using Hot Start Ampliwax PCR Gems 100, Perkin Elmer). 30 cycles of PCR were performed by following the manufacturer's instructions, with the following input conditions: lower reaction, 80° C., 5 min., then add upper reaction and template, 96° C., 1 min. Each reaction vial contained 50 ng template, 0.5 μM each primer, 200 μM dNTPs and 5U (2 μl) Pfu polymerase (Stratagene, LaJolla, Calif.). 30 repeating cycles of: 96° C., 45° sec; 50° C., 45 sec; 75 C, 1 min. A final incubation of 75° C., 10 min, then hold at 4° C. After amplification, the reactions were purified using Qiaquick PCR Purification Kits (Qiagen). The PCR products were digested with Pac1, heat inactivated (65° C., 20 min) and ligated together using T4 DNA ligase (overnight at 4° C. in a 5 μl vol). The ligated DNA was transfected into SCS110 E. coli cells (Stratagene) with kanarnycin (50 μg/ml) antibiotic added to the agar plates. The cells were dcm⁻, dam⁻ (to prevent methylation of Bpm1 sites). The resulting plasmid, pVLBPGN (SEQ ID NO:1, FIGS. 2 & 3) has a deletion in the U3 region of the LTR. A linker containing a central Pac1 site flanked by two outwardly-digesting Bpm1 sites occupies the site of the deleted U3 sequences. The Bpm1 sites enable the plasmid to be digested with Bpm1, resulting in two 2 bp 3′-overhanging ends that are complementary to the U3-derived RT-PCR inserts described below. The digested plasmid was purified free from the intervening linker sequences from an agarose gel after digestion with Bpm1, using the Qiaquick gel purification kit (Qiagen).

Procedure for Amplification of Liver U3 Promoter Region

Purified mouse liver total tissue RNA was purchased from Ambion, Inc., (Austin, Tex.). Total liver RNA was treated with RQ1 Rnase-free (Promega, Madison, Wis.). Using Perkin Elmer Gene Amp thermostable rTth reverse transcriptase RNA PCR kit (P/N N808-0069), the following conditions for RT-PCR were used: RT-PCR A 70° (hot start); RT-PCR B, 95° C., 60 sec, then 35 cycles (95° m 10 sec, 58° C., 15 sec) then a final 58° C. incubation for 7 min, then 4° C. and hold. Additional conditions were: primer concentration 0.15 micromolar, template 100 ng/reaction, dNTPs 200 micromolar (final) and MgCL₂ 3.5 mM(final). The primers for insert amplification were SEQ ID NOS:28 and 68)

The amplified U3 sequences were purified using Qiaquick. The pVLBPGN plasmid was digested with Bpm1, isolated from a 1% agarose gel and purified using the Qiaquick method. The purified U3 sequences were ligated at 1:2, 1:4 and 1:6 molar ratios of VLBPGN plasmid:insert using T4 DNA ligase and a 5 microliter reaction volume overnight at 4° C. (100 ng plasmid: 16 ng insert=1:1 molar ratio). 1 microliter of each ligation reaction was transformed into E. coli SCS 110 competent cells (Stratagene). 26 colonies were recovered in total. Out of 23 clones grown overnight in the presence of kanamycin, 20 had sequences that appeared to be mouse VL30 sequences, representing 10 different VL30 species (FIG. 6, SEQ ID NOS:4-13). One of these (Hep 10, SEQ ID NO: 13) was transiently transfected into Hep G2 liver hepatocellular carcinoma cells. 48 h after transfection, intense GFP fluorescence was observed, indicating strong expression of the Hep 10 U3 promoter region.

EXAMPLE 4 Creating a Combinatorial Library of Mouse VL30 U3 Sub-regions

Using FIG. 7 and Hodgson, 1996, supra, FIG. 4.2 as a guide, the following three sub-regions of the VL30 U3 region were empirically established: Distal (1); medial (2); and proximal (3). Peaks of similarity were used to guide the following choice of primers: (+) primer binding site-5′-LTR boundary; ˜80 bp (defines sub-region 1); ˜80-210 bp (sub-region 2); ˜210-430 (sub-region 3). The following primers were selected to amplify the vector VLBPGN or a similar VL30, NVL-3 LTR-containing vector:

P1 (going left from the 5′-end of the LTR to amplify the plasmid)

(SEQ ID NO:55)

GACTAACCTTGATTCCACTGGAGTTTT(CT)(CT)ATTCTTCATTCCCCACTTC TTCTT

P2 (going right from the 3′-end of the promoter region to amplify the plasmid)

(SEQ ID NO:56)

GACTAACCTTGATTCCACTGGAGAATCTGGACCAATTCTATATAAGCCTG TGAAAAATTT

The six primers selected to amplify the inserts are as follows:

Fragment 1, primer 1 (going right from the LTR terminus into U3) (SEQ ID NO:57) GACTAACCTTGATTCCACTGGAGAAGAAGAAGTGGGGAATGAAGAA

Fragment 1, primer 2 (going left from the end of fragment 1) (SEQ ID NO:58) GACTAACCTTGATTCCACTGGAGATCTCTAGATGGGAGGGG(GT)(CT)GGG CTC

Fragment 2, primer 1 (going right from the left end of fragment 2) (SEQ ID NO:59) GACTAACCTTGATTCCACTGGAGCTCGGAGCCCACCCCCTCCCATCT

Fragment 2, primer 2 (going left from the right end of fragment 2) (SEQ ID NO:60) GACTAACCTTGATTCCACTGGAGGGAGGCCCTTATCTCAAAAATGTT

Fragment 3, primer 1 (going right from the left end of fragment 3) (SEQ ID NO:61) GACTAACCTTGATTCCACTGGAGTCTAAGAACATTTTTGAGATAAGGGCC T

Fragment 3, primer 2 (going left from the right end of fragment 3) (SEQ ID NO:62) GACTAACCTTGATTCCACTGGAGTCACAGGCTTATATAG(TG)AAA

100 ng of genomic DNA from Mus musculus is used as a template (the mouse genome bears 100-200 copies of VL30 elements). Standard PCR procedures for Pfu polymerase are used. Fragments are amplified 35 rounds of PCR to obtain single-copy genomic DNA amplification. Samples of Qiagen column purified DNA are examined on analytical agarose gels to determine the approximate size. The remainder of each reaction is digested with the appropriate enzyme and run on an acrylamide or agarose gel. The digested fragments are purified by standard gel purification procedures and are ligated to the plasmid fragment at an equimolar ratio of the four PCR fragments (three inserts and one plasmid). The ligation mix is transformed into E. coli SCS1 and is grown on kanamycin. The number of colonies is used to establish the size of the combinatorial library and the pooled colonies are grown in E. coli and the DNA is harvested en masse. A dozes or more colonies are characterized by DNA sequencing to determine the approximate fiAelity of the reaction. A library of 1,000 or more, but preferably 100,000 or more members is used for combinatorial screening procedures.

Screening the Combinatorial Libraries for Expression in Specific Cell Types Using a Replication Defective Helper Virus

The U3 library DNA is transfected into the desired target cells in which expression is desired. Along with the library, approximately 25% of the total DNA should include retroviral helper sequences. The latter sequences can be a helper plasmid (such as pPAM3, Miller et al., U.S. Pat. No. 4,861,719). The virus is amphotropic, permitting it to infect most human cells. The RNA from individual clones that are transcribed in the target cells will be packaged into retroviral virions made by the helper virus, and the virions can be harvested as the cell free filtrate (0.45 mm) from the vector producer cells. This virus (containing the expressed sequences) can be transmitted to fresh target cells that do not contain helper virus. as 48 hours after passage, the DNA form of the transcriptionally active clones will be integrated in the recipient cells, and these transcriptionally active loci will produce more RNA, and protein. After G418 drug selection to increase the proportion of cells expressing the vector sequences, helper virus DNA is again transfected into the recipient cells, transforming them into vector producer cells. The virus from these cells should contain increased amounts of the RNA from clones that are transcriptionally active in those cells. Passage of the virus is continued for two or three rounds to permit recombination and mutation to take place, enhancing the effect of in vitro evolution of promoters. The actual degree of enhancement attainable at each step is illustrated in Table 2 (supra). After several passages, the actual level of RNA expressed by several clones is determined by RNA blotting, or by the amount of a reporter gene expressed as protein (determined visually or by the appropriate assay). Because human cells do not naturally contain VL30 DNA or RNA, the sequences that remain in the human cells are those with the most transcriptionally active promoters. These sequences can be amplified and re-cloned using the methods of the instant invention, or they can be rescued by virus packaging, reverse transcribed by the endogenous reverse transcriptase reaction, and grown as plasmids (due to their plasmid origin of replication and the selectable kanamycin marker gene).

In addition to using a replication defective helper virus, such as the clone pPAM3, it is also possible to use a replicatien competent retrovirus, such as Moloney murine leukemia virus to passage the library. For use in human cells, however, the virus should have a tropism that is compatible with human cells (gibbon ape leukemia virus and amphotropic [4070A] murine retroviruses are acceptable).

In addition to being useful for generating active transcriptional promoters de novo, a small variation on the above procedures may enable the isolation of hormone responsive promoters. In it, the cells are treated with the hormone (which could be a steroid, a peptide hormone known to affect the cells, a drug, a drug agonist or antagonist, etc.) during passage. After isolation of surviving VL30 vector-containing cells, individual clones of drug resistant cells are tested for reporter gene expression with and without drug treatment to determine relative protein expression. Likewise, RNA expression can be determined by blot analysis or a similar method. A useful list of known VL30 responses to pharmacological agents is listed in FIG. 4.2 of Hodgson, 1996, supra, and can be used as a guide to help assess the potential agents known to have an effect on VL30 transcription.

Once the transcriptional promoters with the known specificity have been obtained, they can be used to obtain expression of genes from a variety of types of vectors. For example, in addition to retrovirus particles, the promoters can be incorporated into all other major groups of vectors: adenoviruses herpes simplex virus vectors, DNA transfection vectors, etc. It will be apparent to persons of ordinary skill in the art that similar combinatorial libraries can also be used to screen for other characteristics than transcription activity in a particular cell. For example, combinatorial libraries of complementarity determining regions (CDRs) of antibodies or T cell receptors can be so screened using antibody screening methods, such as the phage display screening method (Pharmacia, Milwaukee, Wis.). Thus, the methods of this invention, particularly the combinatorial simplicity of this invention is a significant improvement over many in vivo recombination methods including those of (Stemmer, U.S. Pat. No. 5,605,793, 1997) that have described for the production of CDR combinatorial libraries.

EXAMPLE 5 Gene Assembly Line

From the above examples of 3 and 6 fragment gene self-assemblies, it is evident that assembly of genes by means of gene amplification, the use of offset restriction enzymes and incorporating unique, non-palindromic ends is a highly efficient process compared to conventional cloning methods, However, in addition to the considerations already discussed, it will be apparent to a person of ordinary skill in the art that the various procedures, protocols, methods and material of the instant invention become more difficult to use as the number of fragments increases. For example, if the efficiency of combining each fragment in an assemblage is 99%, then the overall efficiency of combining ten fragments will be 90%, the efficiency of combining 100 fragments will be 37%, etc. Therefore, a small drop in efficiency of any step or fragment, or a large increase in the complexity of the project, will be sufficient to reduce the overall efficiency. Fastidious procedures permit one to achieve success with more complex projects.

Foremost in its potential for inducing failure is human error in primer design where large numbers of fragments are used. Fortunately, the instant invention is suited to automation of most of the steps. This allows human input to be focused on design, analysis, and quality control. For the purposes of generating large vectors or chromosomes, it is desirable to provide an automated environment. One method to achieve this goal is a gene assembly line.

In a gene assembly line, multiple tasks are controlled by a machine or machines working together to increase speed and efficiency and to reduce human error. For example, computer aided design (CAD) and computer aided manufacturing (CAM) are incorporated and combined with the methods of this invention. The computers accept inputs in the form of template and primer sequences, together with preferences of regions to be copied and joined. The preferences include at least the sequences of the primer regions and information about the known restriction sites and maps of the sequences to be assembled, but ideally include the entire sequence. The preferences also include the number of sequences to be joined, the desired Tm for the primers, the list of potential restriction enzymes capable of offset digestion that are potential candidats for use in the assembly process, the desired end structures for each fragment terminus, a tag sequence (if any), whether circular or linear ends are desired, and additional design considerations. The computer algorithm then searches the sequences to determine the candidate enzymes and specific primers that match the criteria of the input. Candidates for selection of unique non-palindromic overlaps are selected. The computer then posts selections or preferences for the type and order of end structures, the primer binding sites, their potential for primer-dimer and intra-molecular interaction artifacts, and the potential conflicts with repeat sequences within the templates that could lead to incorrect polymerization. Based upon the selections made by the operator, the computer then determine the T_(m) for each primer, and makes adjustments (with suitable inputs from the investigator) to achieve a suitable T_(m) for the appropriate DNA synthesis or gene amplification reaction. Ideally, the primers should have similar T_(m)s so that all amplification reactions can be performed at once with one set of amplification instructions. In reality, it may be difficult to do this with complex projects. The output of this portion of the program, which can be in a generic format, such as a Microsoft Excel spreadsheet is then downloaded to a computerized oligonucleotide synthesizer, such as the Applied Biosystems 3928 nucleic acid synthesizer. One advantage of using a computerized synthesizer is its robotic capability to de-protect and purify the oligonucleotides automatically. In addition this synthesizer can accept computerized input.

The quantity of individual oligos recovered is then determined spectrophotometrically. It is desirable to purify the oligonucleotides by high performance liquid chromatography or by polyacrylamide gel. In a preferred embodiment, the oligonucleotides and templates are then assembled robotically using an automated nucleic acid handling system such as the Qiagen Biorobot 9600. The BioRobot is capable of accepting input from a computer and can combine the gene amplification reactions based upon the assignments of templates, primer land reagents provided in the input. The assembled reactions are then amplified for example by PCR. In a preferred embodiment, the PCR heat block is incorporated into the robotic workspace and genes are assembled robotically but with minimal human intervention to change buffers, rearrange the platform, change programs, and the like. The resulting amplified products are also purified by the BioRobot or a similar robotic device. In a preferred embodiment, the robotic device uses Qiaquick cleanup procedures, or a similar method and then assembles restriction endonuclease reactions to digest the purified gene amplification products. The gene amplification products are loaded onto a gel and electrophoresed. Human intervention may be necessary to analyze the products and excise the correct fragments from the gel. At this point, the results are assessed and missing or incorrect sized fragments are resynthesized. The robotic device is preferably used to purify the gel fragments using Qutagen or similar cleanup procedures. After spectrophotometric quantitation of the purified fragments, the robotic device is preferably used to assemble the ligation. Ideally the fragments are combined in an equimolar ratio of 1:1. However it is not necessary to use equimolar ratios in order to achieve gene self-assembly. For automated gene assembly, it may be desirable not to use equimolar ratios of input fragments, particularly if it simplified the task of quantitation. After ligation, the assemblies can be purified and ethanol precipitated or they can be added to the appropriate host cells. Automation aids in maintaining the sterility of the reaction.

Several additional considerations can assist in the construction of long genes using gene assembly. First the number of fragments and the length of constructs are limiting factors. In addition to maintaining high standards of purify of both the oligonucleotide primers and gene amplification products, it is important to keep the error rate low during copying. Thus, one can optimally start with 100 ng of template use only five rounds of gene amplification and finish with nearly 2 micrograms of product. This is more desirable for reducing errors than using a large number of amplification steps. It is also desirable to use a special copying enzyme such as Pfu DNA polymerase that has a low intrinsic error rate. Further it is desirable to use in vivo selection (in eukaryotic cells or tissues) rather than E. coli cloning to reduce the incorporation of errors into the vectors. For example, a viral vector such as an adenoviral vector or the retro-vectors of the preceding examples are auto-selecting. A single correctly-assembled adenovirus vector molecule, for example, leads to a lytic infection (the viral products of which are cloned by limiting dilution on the appropriate eukaryotic cells), even though it may be combined in a ligation mix with a large excess of incorrectly assembled molecules that are non-functional. Thus, it is not necessary to have a high efficiency, although high efficiency has been demonstrated in this system, in order to achieve success in making, for example gene therapy vectors.

For long fragments (3-30 kb), it is desirable to use enzymes and procedures that are designed or facilitate replication of long fragments, one such example is the eLONGase system (Life Technologies). This system can copy up to 30 kb on a fragment with proofreading. Considerations for long PCR are reviewed in Beck, 1998. (The Scientist, Jan. 5, 1998, pp. 16-18).

Internal restriction sites are a potential problem, particularly with large constructs and can be overcome in a number of ways. Use of alternate enzymes, methylation of internal restrictions sites (such as by using methylated DNA precursors during synthesis to leave the sites in primers unaffected, incorporation of the internal sites into the construct (if they are non-palindromic), or mutagenesis of internal sites, are exemplary ways to deal with some of these issues.

For very large constructs, it is desirable to use enzymes such as SapI (recognizing 7 nucleotides and leaving a 3 bp overhang). This enzyme digests every 16,384 bp on average. There are 64 nucleotide triplet combinations, meaning that up to 32 fragments can be ligated in a circle using SapI. Fok1 and Hga1 are other examples of class IIS enzymes that are useful for making large constructs. Hga1 has 5 bp overhangs, permitting more than 500 Hga1 fragments to be ligated. Fok1 includes a Kozak ATG start codon. In a preferred embodiment, a Fok1 site is inserted at the PuXXATG start site of a cDNA encoding region. The cDNA is inserted in frame, providing a site for inserting and switching coding sequences within a vector.

It will be readily understood by those skilled in the art that the foregoing description has been for purposes of illustration only and that a variety of embodiments can be envisioned without departing from the scope of the invention. Therefore, it is intended that the invention not be limited except by the claims.

74 6225 base pairs nucleic acid single linear DNA (genomic) 1 TGAAGAATAA AAAATTACTG GCCTCTTGTG AGAACATGAA CTTTCACCTC GGAGCCCACC 60 CCCTCCCATC TGGAAAACTC CAGTTATAAC TGGAGTTTTT CCTTTAAAAG CTTGTGAAAA 120 ATTTGAGTCG TCGTCGAGAC TCCTCTACCC TGTGCAAAGG TGTATGAGTT TCGACCCCAG 180 AGCTCTGTGT GCTTTCTGTT GCTGCTTTAT TTCGACCCCA GAGCTCTGGT CTGTGTGCTT 240 TCATGTCGCT GCTTTATTAA ATCTTACCTT CTACATTTTA TGTATGGTCT CAGTGTCTTC 300 TTGGGTACGC GGCTGTCCCG GGACTTGAGT GTCTGAGTGA GGGTCTTCCC TCGAGGGTCT 360 TTCATTTGGT ACATGGGCCG GGAATTCGAG AATCTTTCAT TTGGTGCATT GGCCGGGAAT 420 TCGAAAATCT TTCATTTGGT GCATTGGCCG GGAAACAGCG CGACCACCCA GAGGTCCTAG 480 ACCCACTTAG AGGTAAGATT CTTTGTTCTG TTTTGGTCTG ATGTCTGTGT TCTGATGTCT 540 GTGTTCTGTT TCTAAGTCTG GTGCGATCGC AGTTTCAGTT TTGCGGACGC TCAGTGAGAC 600 CGCGCTCCGA GAGGGAGTGC GGGGTGGATA AGGATAGACG TGTCCAGGTG TCCACCGTCC 660 GTTCGCCCTG GGAGACGTCC CAGGAGGAAC AGGGGAGGAT CAGGGACGCC TGGTGGACCC 720 CTTTGAAGGC CAAGAGACCA TTTGGGGTTG CGAGATCGTG GGTTCGAGTC CCACCTCGTG 780 CCCAGTTGCG AGATCGTGGG TTCGAGTCCC ACCTCGTGTT TTGTTGCGAG ATCGTGGGTT 840 CGAGTCCCAC CTCGCGTCTG GTCACGGGAT CGTGGGTTCG AGTCCCACCT CGTGTTTTGT 900 TGCGAGATCG TGGGTTCGAG TCCCACCTCG CGTCTGGTCA CGGGATCGTG GGTTCGAGTC 960 CCACCTCGTG CAGAGGGTCT CAATTGGCCG GCCTTAGAGA GGCCATCTGA TTCTTCTGGT 1020 TTCTCTTTTT GTCTTAGTCT CGTGTCCGCT CTTGTTGTGA CTACTGTTTT TCTAAAAATG 1080 GGACAATCTG TGTCCACTCC CCTTTCTCTG ACTCTGGTTC TGTCGCTTGG TAATTTTGTT 1140 TGTTTACGTT TGTTTTTGTG AGTCGTCTAT GTTGTCTGTT ACTATCTTGT TTTTGTTTGT 1200 GGTTTACGGT TTCTGTGTGT GTCTTGTGTG TCTCTTTGTG TTCAGACTTG GACTGATGAC 1260 TGACGACTGT TTTTAAGTTA TGCCTTCTAA AATAAGCCTA AAAATCCTGT CAGATCCCTA 1320 TGCTGACCAC TTCCTTTCAG ATCAACAGCT GCCCTTACTC GAGCTCAAGC TTCGAATTCT 1380 GCAGTCGACG GTACCGCGGC CGCTAACTAA TAGCCCATTC TCCAAGGTAC GTAGCGGGGA 1440 TCAATTCCGC CCCCCCCCTA ACGTTACTGG CCGAAGCCGC TTGGAATAAG GCCGGTGTGC 1500 GTTTGTCTAT ATGTTATTTT CCACCATATT GCCGTCTTTT GGCAATGTGA GGGCCCGGAA 1560 ACCTGGCCCT GTCTTCTTGA CGAGCATTCC TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT 1620 GCAAGGTCTG TTGAATGTCG TGAAGGAAGC AGTTCCTCTG GAAGCTTCTT GAAGACAAAC 1680 AACGTCTGTA GCGACCCTTT GCAGGCAGCG GAACCCCCCA CCTGGCGACA GGTGCCTCTG 1740 CGGCCAAAAG CCACGTGTAT AAGATACACC TGCAAAGGCG GCACAACCCC AGTGCCACGT 1800 TGTGAGTTGG ATAGTTGTGG AAAGAGTCAA ATGGCTCTCC TCAAGCGTAT TCAACAAGGG 1860 GCTGAAGGAT GCCCAGAAGG TACCCCATTG TATGGGATCT GATCTGGGGC CTCGGTGCAC 1920 ATGCTTTACA TGTGTTTAGT CGAGGTTAAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA 1980 CGTGGTTTTC CTTTGAAAAA CACGATACGG GATCCACCGG TCGCCACCAT GGGTAAAGGA 2040 GAAGAACTTT TCACAGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA TGTTAATGGG 2100 CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA ACTTACCCTT 2160 AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGC CAACACTTGT CACTACTTTC 2220 ACTTATGGTG TTCAATGCTT TTCAAGATAC CCAGATCATA TGAAACGGCA TGACTTTTTC 2280 AAGAGTGCCA TGCCCGAAGG TTATGTACAG GAAAGAACTA TATTTTTCAA AGATGACGGG 2340 AACTACAAGA CACGTGCTGA AGTCAAGTTT GAAGGTGATA CCCTTGTTAA TAGAATCGAG 2400 TTAAAAGGTA TTGATTTTAA AGAAGATGGA AACATTCTTG GACACAAATT GGAATACAAC 2460 TATAACTCAC ACAATGTATA CATCATGGCA GACAAACAAA AGAATGGAAC CAAAGTTAAC 2520 TTCAAAATTA GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA TTATCAACAA 2580 AATACTCCAA TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT GTCCACACAA 2640 TCTGCCCTTT CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT TGAGTTTGTA 2700 ACAGCTGCTG GGATTACACA TGGCATGGAT GAACTATACA AGTCCGGATC TAGATAACTG 2760 TATCGATGGA TCCGAAGGCG GGGACAGCAG TGCAGTGGTG GACAGAAAGC AAGTGATCTA 2820 GGCCAGCAGC CTCCCTAAAG GGACTTCAGC CCACAAAGCC AAACTTGTGG CTTTAATACA 2880 AGCTCTGTAA ATGGTAAAAA AAAAAAAGTC TACACGGACA GCAGGTATGC TCTTGCCACT 2940 GTACAGAGCA ATATACAGAC AAAGAGAACT GTTGACATCT GCAGAGAAAG ACCTAAGATG 3000 CTGTGGCTAA AAGAAATCAG ATGGCAAATC TAACCGCCCA GGCATCCTAA AGAGCAATGA 3060 TCCTGACAGT CTGAAGACTA TCAAGTTATA GACAAATTAA GACTGGTAAA AAAAACCCTG 3120 TATAAAATAG TAAAAACTGA AAAAAGAAAA CTAGTCCTCT CATGAGAAGA CAGACCTGAC 3180 ATCTACTGAA AAATAGACTT TACTGGAAAA AATATGTGTA TGAATACCTT CTAGTTTTTG 3240 TGAACGTTCT CAAGATGGAT AAAAGCTTTT CCTTGTAAAA CGAGACTGAT CAGATAGTCA 3300 TCAAGAAGAT TGTTAAAGAA AATTTTCCAA GGTTCGGAGT GCCAAAAGCA ATAGTGTCAG 3360 ATAATGGTCC TGCCTTTGTT GCCCAGGTAA GTCAGGGTGT GGCCAAGTAT TTAGAGGTCA 3420 AATGAAAATT CCATTGTGTG TACAGACCTC AGAGCTCAGG AAAGATAAAA AAGAATAAAT 3480 AAAACTCTAA ACAGACCTTG ACAAAATTAA TCCTAGAGAC TGGCACAGAC TTACTTGGTA 3540 CTCCTTCCCC TTGCCCTATT TAGAACTGAG AATACTCCCT CTTGATTCGG TTTTACTCTT 3600 TTTAAGATCC TTTATGGGGC TCCTATGCCA TCACTGTCTT AAATGATGTG TTTAAACCTA 3660 TGTTGTTATA ATAATGATCT ATATGTTAAG TTAAAAGGCT TGCAGGTGGT GCAGAAAGAA 3720 GTCTGGTCAC AACTGGCTAC AGTGAACAAG CTGGGTACCC CAAGGACATC TTACCAGTTC 3780 CAGCCAGAGA TCTGATCTAC GATCCCCGGG TCGACCCGGG TCGACCCTGT GGAATGTGTG 3840 TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA 3900 TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT 3960 GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC 4020 GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT 4080 TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT 4140 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT TCACGCTGCC GCAAGCACTC AGGGCGCAAG 4200 GGCTGCTAAA GGAAGCGGAA CACGTAGAAA GCCAGTCCGC AGAAACGGTG CTGACCCCGG 4260 ATGAATGTCA GCTACTGGGC TATCTGGACA AGGGAAAACG CAAGCGCAAA GAGAAAGCAG 4320 GTAGCTTGCA GTGGGCTTAC ATGGCGATAG CTAGACTGGG CGGTTTTATG GACAGCAAGC 4380 GAACCGGAAT TGCCAGCTGG GGCGCCCTCT GGTAAGGTTG GGAAGCCCTG CAAAGTAAAC 4440 TGGATGGCTT TCTTGCCGCC AAGGATCTGA TGGCGCAGGG GATCAAGATC TGATCAAGAG 4500 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 4560 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 4620 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 4680 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 4740 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 4800 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 4860 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 4920 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGGG 4980 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 5040 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 5100 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 5160 GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 5220 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 5280 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA 5340 CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG 5400 AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGGAATTCG TAATCTGCTG CTTGCAAACA 5460 AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT 5520 CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG 5580 TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC 5640 CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA 5700 CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC 5760 AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCA TTGAGAAAGC 5820 GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA 5880 GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG 5940 TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA 6000 TGGAAAAACG CCAGCAACGC CGAGATGCGC CGCCTCGAGT ACACCTGCGT CATGCTGAGA 6060 CCCTCAAGCC TCACTAAAAG GGTCCCTGCC TAGTTCTGTT TACTAATCTG CCTTATTCTG 6120 TTTTTGTTCC CATGTTAAAG ATAGAGTAAA TGCAGTATTC TCCACATAGA GATATAGACT 6180 TCTGAAATTC TAAGATTAGA ATTATTTACA AGAAGAAGTG GGGAA 6225 487 base pairs nucleic acid single linear DNA (genomic) 2 CCTCCCATCT AGAGGTTGTT CTCGGAACAC TCCTAAACTT TTCACCCCAA AACTCCTCAC 60 CCTAAAGTTC GAAAAAACTG TTCCAAGAAC ATTTTTGAGA TAAAGGCCTC CTAGAACAAC 120 CTCAAAATGA CATTGCCAAA TGATAAGACA TGACTCCTTA GTTACGTAGG TTCCTTGATA 180 GGACATGACT CCTTAGTTAC GTAGGTTCCT TGATAGGACA TGACTCCTTA GTTACGTAGA 240 TTCCTTTGGT AGAACTCCCT AGTGATGTAA ACTTGTACTT TCCCTGCCCA GTTCTCCCCC 300 TTTGAGTTTT ACTATATAAG CCTGTAAAAA ATTTTTGCTG ACCGTCGAGA CTCCTCTACC 360 CTGTGCTAAG GTGTATGAGT TTCGACCCCA GAGCTCTGTG TGCTTCCATG TTGCTGCTTT 420 ATTTCGACCC CAGAGCTCTG GTCTGTGTGC TTTCATGTCG CTGCTTTATT AAATCTTGCC 480 TTCTACA 487 366 base pairs nucleic acid single linear DNA (genomic) 3 CCTCCCATCT AGAAAACATT TTTGAGATAA AGGCTTCCTG GAACAACCTC AAAATGAACC 60 AGGTACTCCT TAGTTACGTA GGTTCCTTGA TAGGACATGA CTCCTTAGTT ACATAGATTC 120 CTTTGGCAGA ACTCCCTAGT GATGTAAACT TGTACTTTCC CTGCCCAGTT CTCCCCCTTT 180 GAGTTTTACT ATATAAGCCT GTGAAAAATT TTGGCTGACC GTCGAGACTC CTCTACCCTG 240 TGCTAAGGTG TATGAGTTTC GACCCCAGAG CTCTGTGTGC TTCCATGTTG CTGCTTTATT 300 TCGACCCCAG AGCTCTGGTC TGTGTGCTTT CATGTTGCTG CCTTATTAAA TCTTGCCTTC 360 TACATT 366 304 base pairs nucleic acid single linear DNA (genomic) 4 CCTCCCATCT AGAGATTGTT CCCAGAACAC TCCTGAACTC TTCACCCCAG AATGCATGCC 60 TGAACTCCTC ACCCTAGAGT TCGAACCCTC CCAACTAAAG ACTGTTCCAA GAACATTTTT 120 GAGATAAGGG CCTCCTGGAA CAACCTCAGA ATGAACCGGG TACATTGCCA AATAATAGGA 180 CATGACCCCT TAGTTACGTA AAATCCCTTG GCAGAACCCC TTGTCCCTTG GCAGAACCCC 240 TTAGTTATGT AAACTTGTAC TTTCCCTACC CCGCTCTCCC CCCTTGAGTT TTTCCTATAT 300 AAGC 304 304 base pairs nucleic acid single linear DNA (genomic) 5 CCTCCCATCT AGAGAGTGTT CCCAGAACAC TCCTGAACTC TTCACCCCAG AATGCATTCC 60 TGAACTCCTC ACCCTAGAGT TCGAACCCTC CCAACTAAAG ACTGTTCCAA GAACATTTTT 120 GAGATAAGGG CCTCCTGGAA CAACCTCAGA ATGAACCGGA TACATTGCCA AATAATAGGA 180 CATGACCCCT TAGTTACGTA GAATCCCTTG GCAGAACCCC TTGTCCCTTG GCAGAACCCC 240 TTAGTTATGT AAACTTGTAC TTTCCCTACC CCGCTCTCCC CCCTTGAGTT TTTCCTATAT 300 AAGC 304 304 base pairs nucleic acid single linear DNA (genomic) 6 CCTCCCATCT AGAGAGTGTT CCCAGAACAC TCCTGAACTC TTCACCCCAG AATGCATTCC 60 TGAACTCCTC ATCCTAGAGT TCGAACCCTC CCAACTAAAG ACTGTTCCAA GAACATTTTT 120 GAGATAAGGG CCTCCTGGAA CAACCTCAGA ATGAACCTGG TACATTGCCA AATAATAGGA 180 CATGACCCTT TAGTTACGTA GAATCCCTTG GCAGAACCCC TTGTCCCTTG GCAGAACCCC 240 TTAGTTATGC AAACTTGTAC TTTCTCTGCC CCGCTCTCCC CCCTTGAGTT TTTCCTATAT 300 AAGC 304 304 base pairs nucleic acid single linear DNA (genomic) 7 CCTCCCATCT AGAGAGTGTT CCCAGAACAC TCCTGAACTC TTCACCTCAA AATGCATTCC 60 TGAACTCCTC ACCCTAGAGT TCGAACCCTC CCAACTAAAG ACTGTTCCAA GAACATTTTT 120 GAGATAAGGG CCTCCTGGAA CAACCTCAGA ATGAACCAGG TACATTGCCA AATAATAGGA 180 CATGACCCTT TAGTTACGTA GAATCCCTTG GCAGAACCCC TTGTCCCTTG GCAGAACCCC 240 TTAGTTATGC AAACTTGTAC TTTCTCTGCC CCGCTCTCCC CCCTTGAGTT TTTCCTATAT 300 AAGC 304 305 base pairs nucleic acid single linear DNA (genomic) 8 CCTCCCATCT AGAGATTGTT CCCAGAACAC TCCTGAACTC TTCACCCCAG AATGCATTCC 60 TGAACTCCTC ACCCTAGAGT TCGAACCCTC CCAACTAAAG ACTGTTCCAA GAACATTTTT 120 GAGATAAGGG CCTCCTGGAA CAACCTCAGA ATGAACCGGA TACATTGCCA AATAATAGGA 180 CATGACCCCT TAGTTACGTA GAATTCCCTT GGCAGAACCC CTTGTCCCTT GGCAGAACCC 240 CTTAGTTATG CAAACTTGTA CTTTCCCTGC CCCGCTCTCC CCCCTTGAGG TTTTCCTATA 300 TAAGC 305 305 base pairs nucleic acid single linear DNA (genomic) 9 CCTCCCATCT AGAGAGTGTT CCCAGAACAC TCCTGAACTC TTCACCCCAG AATGCATTCC 60 TGAACCCCTC ACCCTAGAGT TCGAACCCTC CCAACTAAAG ACTGTTCCAA GAACATTTTT 120 GAGATAAGGG CCTCCTGGAA CAACCTCAGA ATGAACCAGG TACATTGCCA AATAATAGGA 180 CATGACCCCT TAGTTACGTA GAATTCCCTT GGCAGAACCC CTTGTCCCTT GGCAGAACCC 240 CTTAGTTATG CGAACTTGTA CTTTCCCTGC CCCGCTCTCC CCCCTTGAGT TTTTCCTATA 300 TAAGC 305 306 base pairs nucleic acid single linear DNA (genomic) 10 CCCTCCCATC TAGAGAGTGT TCCCAGAACA CTCCTGAACT CTTCATCCCA GAATGCATTC 60 CTGAACTCCT CACCCTATAG TTCGAACCCT CCCAACTAAA GACTGTTCCA AGAACATTTT 120 TGAGATAAGG GCCTCCTGGA ACAACCTCAG AATGAACCGG GTACATTGCC AAATAATAGG 180 ACATGACCCC TTAGTTACGT AGAATTCCCT TGGCAGAACC CCTTGTCGCT TGGCAGAACC 240 CCTTAGTTAT GTAAACTTGT ACTTTCCCTG CCCCGCTCTC CCCCCTTGAG TTTTTACTAT 300 ATAAGC 306 305 base pairs nucleic acid single linear DNA (genomic) 11 CCTCCCATCT AGAGAGTGTT CCCAAAACAC TCCTGAACTC TTCACCCCAG AATGCATTCC 60 TGAACTCCTC ACCCTAAAGT TCAAACCCTC CCAACTAAAG ACTGTTCCAA GAACATTTTT 120 GAGATAAGGG CCTCCTGGAA CAACCTCAGA ATGAACCGGG TACATTGCCA AATAATAGGA 180 CATGACCCCT TAGTTACACA GAATTCCCTT GGCAAAACCC CTTGTCCCTT GGCAGAACCC 240 CTTAGTTATG CAAACTTGTA CTTTCCCTGC CCAGCTCTCC CCCCTTGAGT TTTTCCTATA 300 TAAGC 305 304 base pairs nucleic acid single linear DNA (genomic) 12 CCTCCCATCT AGAGAGTGTT CCCAGAACAC TCCTGAACTC TTCACCCCAG AATGCATTCC 60 TGAACTCCTC ACCCTAGAGT TTGAACCCTC CCAACTAAAG ACTGTTCCAA GAACATCTTT 120 GAGATAAGGG CCTCCTGGAA CAACCTCAGA ATGAACCGGG TACATTGCCA AATAATAGGA 180 CATGACCCCT TAGTTACGTA GAATTCCCTT GGCAGAACCC CTTGTCGCTT GGCAGAACCC 240 CTTAGTTATG CAAACTTGTA CTTTCCCTGC CCCGCTCTCC CCCTTGAGTT TTTCCTATAA 300 AAGC 304 303 base pairs nucleic acid single linear DNA (genomic) 13 CCTCCCATCT AGAGAGTGTT CCCAGAACAC TCCTAAACTC TTCACCCCAG AATGCATTCC 60 TGAACTCCTC ACCCTAGAGT TCGAACCCTT CCAACTAAAG ACTGTTCCAA GAACATTTTT 120 GAGATAAGGG CCTCCTGGAA CAACCTCAAA ATGAACCGGG TACATTGCCA AATGATAGGA 180 CATGACCCCT TAGTTACGTA GATTCCCTTG GCAGAACCCC TTGTCCCTTG GCAGAACCCC 240 CTAGTGATGT AAACTTGTAC TTTCCCTGCC CAGCTCTCCC CCCTTGAGTT TTCCTATATA 300 AGC 303 8657 base pairs nucleic acid single linear DNA (genomic) 14 TGAAGAATAA AAAATTACTG GCCTCTTGTG AGAACATGAA CTTTCACCTC GGAGCCCACC 60 CCCTCCCATC TGGAAAACAT ACTTGAGAAA AACATTTTCT GGAACAACCA CAGAATGTTT 120 CAACAGGCCA GATGTATTGC CAAACACAGG ATATGACTCT TTGGTTGAGT AAATTTGTGG 180 TTGTTAAACT TCCCCTATTC CCTCCCCATT CCCCCTCCCA GTTTGTGGTT TTTTCCTTTA 240 AAAGCTTGTG AAAAATTTGA GTCGTCGTCG AGACTCCTCT ACCCTGTGCA AAGGTGTATG 300 AGTTTCGACC CCAGAGCTCT GTGTGCTTTC TGTTGCTGCT TTATTTCGAC CCCAGAGCTC 360 TGGTCTGTGT GCTTTCATGT CGCTGCTTTA TTAAATCTTA CCTTCTACAT TTTATGTATG 420 GTCTCAGTGT CTTCTTGGGT ACGCGGCTGT CCCGGGACTT GAGTGTCTGA GTGAGGGTCT 480 TCCCTCGAGG GTCTTTCATT TGGTACATGG GCCGGGAATT CGAGAATCTT TCATTTGGTG 540 CATTGGCCGG GAATTCGAAA ATCTTTCATT TGGTGCATTG GCCGGGAAAC AGCGCGACCA 600 CCCAGAGGTC CTAGACCCAC TTAGAGGTAA GATTCTTTGT TCTGTTTTGG TCTGATGTCT 660 GTGTTCTGAT GTCTGTGTTC TGTTTCTAAG TCTGGTGCGA TCGCAGTTTC AGTTTTGCGG 720 ACGCTCAGTG AGACCGCGCT CCGAGAGGGA GTGCGGGGTG GATAAGGATA GACGTGTCCA 780 GGTGTCCACC GTCCGTTCGC CCTGGGAGAC GTCCCAGGAG GAACAGGGGA GGATCAGGGA 840 CGCCTGGTGG ACCCCTTTGA AGGCCAAGAG ACCATTTGGG GTTGCGAGAT CGTGGGTTCG 900 AGTCCCACCT CGTGCCCAGT TGCGAGATCG TGGGTTCGAG TCCCACCTCG TGTTTTGTTG 960 CGAGATCGTG GGTTCGAGTC CCACCTCGCG TCTGGTCACG GGATCGTGGG TTCGAGTCCC 1020 ACCTCGTGTT TTGTTGCGAG ATCGTGGGTT CGAGTCCCAC CTCGCGTCTG GTCACGGGAT 1080 CGTGGGTTCG AGTCCCACCT CGTGCAGAGG GTCTCAATTG GCCGGCCTTA GAGAGGCCAT 1140 CTGATTCTTC TGGTTTCTCT TTTTGTCTTA GTCTCGTGTC CGCTCTTGTT GTGACTACTG 1200 TTTTTCTAAA AATGGGACAA TCTGTGTCCA CTCCCCTTTC TCTGACTCTG GTTCTGTCGC 1260 TTGGTAATTT TGTTTGTTTA CGTTTGTTTT TGTGAGTCGT CTATGTTGTC TGTTACTATC 1320 TTGTTTTTGT TTGTGGTTTA CGGTTTCTGT GTGTGTCTTG TGTGTCTCTT TGTGTTCAGA 1380 CTTGGACTGA TGACTGACGA CTGTTTTTAA GTTATGCCTT CTAAAATAAG CCTAAAAATC 1440 CTGTCAGATC CCTATGCTGA CCACTTCCTT TCAGATCAAC AGCTGCCCTT ACGTATCGAT 1500 GGATCCCTCG ACTAACTAAT AGCCCATTCT CCAAGGTCGA GCGGGATCAA TTCCGCCCCC 1560 CCCCTAACGT TACTGGCCGA AGCCGCTTGG AATAAGGCCG GTGTGCGTTT GTCTATATGT 1620 TATTTTCCAC CATATTGCCG TCTTTTGGCA ATGTGAGGGC CCGGAAACCT GGCCCTGTCT 1680 TCTTGACGAG CATTCCTAGG GGTCTTTCCC CTCTCGCCAA AGGAATGCAA GGTCTGTTGA 1740 ATGTCGTGAA GGAAGCAGTT CCTCTGGAAG CTTCTTGAAG ACAAACAACG TCTGTAGCGA 1800 CCCTTTGCAG GCAGCGGAAC CCCCCACCTG GCGACAGGTG CCTCTGCGGC CAAAAGCCAC 1860 GTGTATAAGA TACACCTGCA AAGGCGGCAC AACCCCAGTG CCACGTTGTG AGTTGGATAG 1920 TTGTGGAAAG AGTCAAATGG CTCTCCTCAA GCGTATTCAA CAAGGGGCTG AAGGATGCCC 1980 AGAAGGTACC CCATTGTATG GGATCTGATC TGGGGCCTCG GTGCACATGC TTTACATGTG 2040 TTTAGTCGAG GTTAAAAAAA CGTCTAGGCC CCCCGAACCA CGGGGACGTG GTTTTCCTTT 2100 GAAAAACACG ATAATAATCA TGGGCGCGGA TCCCGTCGTT TTACAACGTC GTGACTGGGA 2160 AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG 2220 TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA 2280 ATGGCGCTTT GCCTGGTTTC CGGCACCAGA AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA 2340 TCTTCCTGAG GCCGATACTG TCGTCGTCCC CTCAAACTGG CAGATGCACG GTTACGATGC 2400 GCCCATCTAC ACCAACGTAA CCTATCCCAT TACGGTCAAT CCGCCGTTTG TTCCCACGGA 2460 GAATCCGACG GGTTGTTACT CGCTCACATT TAATGTTGAT GAAAGCTGGC TACAGGAAGG 2520 CCAGACGCGA ATTATTTTTG ATGGCGTTAA CTCGGCGTTT CATCTGTGGT GCAACGGGCG 2580 CTGGGTCGGT TACGGCCAGG ACAGTCGTTT GCCGTCTGAA TTTGACCTGA GCGCATTTTT 2640 ACGCGCCGGA GAAAACCGCC TCGCGGTGAT GGTGCTGCGT TGGAGTGACG GCAGTTATCT 2700 GGAAGATCAG GATATGTGGC GGATGAGCGG CATTTTCCGT GACGTCTCGT TGCTGCATAA 2760 ACCGACTACA CAAATCAGCG ATTTCCATGT TGCCACTCGC TTTAATGATG ATTTCAGCCG 2820 CGCTGTACTG GAGGCTGAAG TTCAGATGTG CGGCGAGTTG CGTGACTACC TACGGGTAAC 2880 AGTTTCTTTA TGGCAGGGTG AAACGCAGGT CGCCAGCGGC ACCGCGCCTT TCGGCGGTGA 2940 AATTATCGAT GAGCGTGGTG GTTATGCCGA TCGCGTCACA CTACGTCTGA ACGTCGAAAA 3000 CCCGAAACTG TGGAGCGCCG AAATCCCGAA TCTCTATCGT GCGGTGGTTG AACTGCACAC 3060 CGCCGACGGC ACGCTGATTG AAGCAGAAGC CTGCGATGTC GGTTTCCGCG AGGTGCGGAT 3120 TGAAAATGGT CTGCTGCTGC TGAACGGCAA GCCGTTGCTG ATTCGAGGCG TTAACCGTCA 3180 CGAGCATCAT CCTCTGCATG GTCAGGTCAT GGATGAGCAG ACGATGGTGC AGGATATCCT 3240 GCTGATGAAG CAGAACAACT TTAACGCCGT GCGCTGTTCG CATTATCCGA ACCATCCGCT 3300 GTGGTACACG CTGTGCGACC GCTACGGCCT GTATGTGGTG GATGAAGCCA ATATTGAAAC 3360 CCACGGCATG GTGCCAATGA ATCGTCTGAC CGATGATCCG CGCTGGCTAC CGGCGATGAG 3420 CGAACGCGTA ACGCGAATGG TGCAGCGCGA TCGTAATCAC CCGAGTGTGA TCATCTGGTC 3480 GCTGGGGAAT GAATCAGGCC ACGGCGCTAA TCACGACGCG CTGTATCGCT GGATCAAATC 3540 TGTCGATCCT TCCCGCCCGG TGCAGTATGA AGGCGGCGGA GCCGACACCA CGGCCACCGA 3600 TATTATTTGC CCGATGTACG CGCGCGTGGA TGAAGACCAG CCCTTCCCGG CTGTGCCGAA 3660 ATGGTCCATC AAAAAATGGC TTTCGCTACC TGGAGAGACG CGCCCGCTGA TCCTTTGCGA 3720 ATACGCCCAC GCGATGGGTA ACAGTCTTGG CGGTTTCGCT AAATACTGGC AGGCGTTTCG 3780 TCAGTATCCC CGTTTACAGG GCGGCTTCGT CTGGGACTGG GTGGATCAGT CGCTGATTAA 3840 ATATGATGAA AACGGCAACC CGTGGTCGGC TTACGGCGGT GATTTTGGCG ATACGCCGAA 3900 CGATCGCCAG TTCTGTATGA ACGGTCTGGT CTTTGCCGAC CGCACGCCGC ATCCAGCGCT 3960 GACGGAAGCA AAACACCAGC AGCAGTTTTT CCAGTTCCGT TTATCCGGGC AAACCATCGA 4020 AGTGACCAGC GAATACCTGT TCCGTCATAG CGATAACGAG CTCCTGCACT GGATGGTGGC 4080 GCTGGATGGT AAGCCGCTGG CAAGCGGTGA AGTGCCTCTG GATGTCGCTC CACAAGGTAA 4140 ACAGTTGATT GAACTGCCTG AACTACCGCA GCCGGAGAGC GCCGGGCAAC TCTGGCTCAC 4200 AGTACGCGTA GTGCAACCGA ACGCGACCGC ATGGTCAGAA GCCGGGCACA TCAGCGCCTG 4260 GCAGCAGTGG CGTCTGGCGG AAAACCTCAG TGTGACGCTC CCCGCCGCGT CCCACGCCAT 4320 CCCGCATCTG ACCACCAGCG AAATGGATTT TTGCATCGAG CTGGGTAATA AGCGTTGGCA 4380 ATTTAACCGC CAGTCAGGCT TTCTTTCACA GATGTGGATT GGCGATAAAA AACAACTGCT 4440 GACGCCGCTG CGCGATCAGT TCACCCGTGC ACCGCTGGAT AACGACATTG GCGTAAGTGA 4500 AGCGACCCGC ATTGACCCTA ACGCCTGGGT CGAACGCTGG AAGGCGGCGG GCCATTACCA 4560 GGCCGAAGCA GCGTTGTTGC AGTGCACGGC AGATACACTT GCTGATGCGG TGCTGATTAC 4620 GACCGCTCAC GCGTGGCAGC ATCAGGGGAA AACCTTATTT ATCAGCCGGA AAACCTACCG 4680 GATTGATGGT AGTGGTCAAA TGGCGATTAC CGTTGATGTT GAAGTGGCGA GCGATACACC 4740 GCATCCGGCG CGGATTGGCC TGAACTGCCA GCTGGCGCAG GTAGCAGAGC GGGTAAACTG 4800 GCTCGGATTA GGGCCGCAAG AAAACTATCC CGACCGCCTT ACTGCCGCCT GTTTTGACCG 4860 CTGGGATCTG CCATTGTCAG ACATGTATAC CCCGTACGTC TTCCCGAGCG AAAACGGTCT 4920 GCGCTGCGGG ACGCGCGAAT TGAATTATGG CCCACACCAG TGGCGCGGCG ACTTCCAGTT 4980 CAACATCAGC CGCTACAGTC AACAGCAACT GATGGAAACC AGCCATCGCC ATCTGCTGCA 5040 CGCGGAAGAA GGCACATGGC TGAATATCGA CGGTTTCCAT ATGGGGATTG GTGGCGACGA 5100 CTCCTGGAGC CCGTCAGTAT CGGCGGAATT CCAGCTGAGC GCCGGTCGCT ACCATTACCA 5160 GTTGGTCTGG TGTCAAAAAT AATAATAACC GGGCAGGGGG GATCCGAAGG CGGGGACAGC 5220 AGTGCAGTGG TGGACAGAAA GCAAGTGATC TAGGCCAGCA GCCTCCCTAA AGGGACTTCA 5280 GCCCACAAAG CCAAACTTGT GGCTTTAATA CAAGCTCTGT AAATGGTAAA AAAAAAAAAG 5340 TCTACACGGA CAGCAGGTAT GCTCTTGCCA CTGTACAGAG CAATATACAG ACAAAGAGAA 5400 CTGTTGACAT CTGCAGAGAA AGACCTAAGA TGCTGTGGCT AAAAGAAATC AGATGGCAAA 5460 TCTAACCGCC CAGGCATCCT AAAGAGCAAT GATCCTGACA GTCTGAAGAC TATCAAGTTA 5520 TAGACAAATT AAGACTGGTA AAAAAAACCC TGTATAAAAT AGTAAAAACT GAAAAAAGAA 5580 AACTAGTCCT CTCATGAGAA GACAGACCTG ACATCTACTG AAAAATAGAC TTTACTGGAA 5640 AAAATATGTG TATGAATACC TTCTAGTTTT TGTGAACGTT CTCAAGATGG ATAAAAGCTT 5700 TTCCTTGTAA AACGAGACTG ATCAGATAGT CATCAAGAAG ATTGTTAAAG AAAATTTTCC 5760 AAGGTTCGGA GTGCCAAAAG CAATAGTGTC AGATAATGGT CCTGCCTTTG TTGCCCAGGT 5820 AAGTCAGGGT GTGGCCAAGT ATTTAGAGGT CAAATGAAAA TTCCATTGTG TGTACAGACC 5880 TCAGAGCTCA GGAAAGATAA AAAAGAATAA ATAAAACTCT AAACAGACCT TGACAAAATT 5940 AATCCTAGAG ACTGGCACAG ACTTACTTGG TACTCCTTCC CCTTGCCCTA TTTAGAACTG 6000 AGAATACTCC CTCTTGATTC GGTTTTACTC TTTTTAAGAT CCTTTATGGG GCTCCTATGC 6060 CATCACTGTC TTAAATGATG TGTTTAAACC TATGTTGTTA TAATAATGAT CTATATGTTA 6120 AGTTAAAAGG CTTGCAGGTG GTGCAGAAAG AAGTCTGGTC ACAACTGGCT ACAGTGAACA 6180 AGCTGGGTAC CCCAAGGACA TCTTACCAGT TCCAGCCAGA GATCTGATCT ACGATCCCCG 6240 GGTCGACCCG GGTCGACCCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 6300 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 6360 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 6420 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 6480 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 6540 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 6600 CTTCACGCTG CCGCAAGCAC TCAGGGCGCA AGGGCTGCTA AAGGAAGCGG AACACGTAGA 6660 AAGCCAGTCC GCAGAAACGG TGCTGACCCC GGATGAATGT CAGCTACTGG GCTATCTGGA 6720 CAAGGGAAAA CGCAAGCGCA AAGAGAAAGC AGGTAGCTTG CAGTGGGCTT ACATGGCGAT 6780 AGCTAGACTG GGCGGTTTTA TGGACAGCAA GCGAACCGGA ATTGCCAGCT GGGGCGCCCT 6840 CTGGTAAGGT TGGGAAGCCC TGCAAAGTAA ACTGGATGGC TTTCTTGCCG CCAAGGATCT 6900 GATGGCGCAG GGGATCAAGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG 6960 AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG 7020 ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGC 7080 GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG 7140 AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG 7200 TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC 7260 TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC 7320 TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC 7380 GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC 7440 AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG 7500 ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT 7560 TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT 7620 TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC 7680 TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT 7740 TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC 7800 ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG 7860 GGACGGAATT CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT 7920 GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC 7980 AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG 8040 TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG 8100 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT 8160 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC 8220 TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG 8280 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG 8340 GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT 8400 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCCGAGATGC 8460 GCCGCCTCGA GTACACCTGC GTCATGCTGA GACCCTCAAG CCTCACTAAA AGGGTCCCTG 8520 CCTAGTTCTG TTTACTAATC TGCCTTATTC TGTTTTTGTT CCCATGTTAA AGATAGAGTA 8580 AATGCAGTAT TCTCCACATA GAGATATAGA CTTCTGAAAT TCTAAGATTA GAATTATTTA 8640 CAAGAAGAAG TGGGGAA 8657 6359 base pairs nucleic acid single linear DNA (genomic) 15 TGAAGAATAA AAAATTACTG GCCTCTTGTG AGAACATGAA CTTTCACCTC GGAGCCCACC 60 CCCTCCCATC TGGAAAACAT ACTTGAGAAA AACATTTTCT GGAACAACCA CAGAATGTTT 120 CAACAGGCCA GATGTATTGC CAAACACAGG ATATGACTCT TTGGTTGAGT AAATTTGTGG 180 TTGTTAAACT TCCCCTATTC CCTCCCCATT CCCCCTCCCA GTTTGTGGTT TTTTCCTTTA 240 AAAGCTTGTG AAAAATTTGA GTCGTCGTCG AGACTCCTCT ACCCTGTGCA AAGGTGTATG 300 AGTTTCGACC CCAGAGCTCT GTGTGCTTTC TGTTGCTGCT TTATTTCGAC CCCAGAGCTC 360 TGGTCTGTGT GCTTTCATGT CGCTGCTTTA TTAAATCTTA CCTTCTACAT TTTATGTATG 420 GTCTCAGTGT CTTCTTGGGT ACGCGGCTGT CCCGGGACTT GAGTGTCTGA GTGAGGGTCT 480 TCCCTCGAGG GTCTTTCATT TGGTACATGG GCCGGGAATT CGAGAATCTT TCATTTGGTG 540 CATTGGCCGG GAATTCGAAA ATCTTTCATT TGGTGCATTG GCCGGGAAAC AGCGCGACCA 600 CCCAGAGGTC CTAGACCCAC TTAGAGGTAA GATTCTTTGT TCTGTTTTGG TCTGATGTCT 660 GTGTTCTGAT GTCTGTGTTC TGTTTCTAAG TCTGGTGCGA TCGCAGTTTC AGTTTTGCGG 720 ACGCTCAGTG AGACCGCGCT CCGAGAGGGA GTGCGGGGTG GATAAGGATA GACGTGTCCA 780 GGTGTCCACC GTCCGTTCGC CCTGGGAGAC GTCCCAGGAG GAACAGGGGA GGATCAGGGA 840 CGCCTGGTGG ACCCCTTTGA AGGCCAAGAG ACCATTTGGG GTTGCGAGAT CGTGGGTTCG 900 AGTCCCACCT CGTGCCCAGT TGCGAGATCG TGGGTTCGAG TCCCACCTCG TGTTTTGTTG 960 CGAGATCGTG GGTTCGAGTC CCACCTCGCG TCTGGTCACG GGATCGTGGG TTCGAGTCCC 1020 ACCTCGTGTT TTGTTGCGAG ATCGTGGGTT CGAGTCCCAC CTCGCGTCTG GTCACGGGAT 1080 CGTGGGTTCG AGTCCCACCT CGTGCAGAGG GTCTCAATTG GCCGGCCTTA GAGAGGCCAT 1140 CTGATTCTTC TGGTTTCTCT TTTTGTCTTA GTCTCGTGTC CGCTCTTGTT GTGACTACTG 1200 TTTTTCTAAA AATGGGACAA TCTGTGTCCA CTCCCCTTTC TCTGACTCTG GTTCTGTCGC 1260 TTGGTAATTT TGTTTGTTTA CGTTTGTTTT TGTGAGTCGT CTATGTTGTC TGTTACTATC 1320 TTGTTTTTGT TTGTGGTTTA CGGTTTCTGT GTGTGTCTTG TGTGTCTCTT TGTGTTCAGA 1380 CTTGGACTGA TGACTGACGA CTGTTTTTAA GTTATGCCTT CTAAAATAAG CCTAAAAATC 1440 CTGTCAGATC CCTATGCTGA CCACTTCCTT TCAGATCAAC AGCTGCCCTT ACTCGAGCTC 1500 AAGCTTCGAA TTCTGCAGTC GACGGTACCG CGGCCGCTAA CTAATAGCCC ATTCTCCAAG 1560 GTACGTAGCG GGGATCAATT CCGCCCCCCC CCTAACGTTA CTGGCCGAAG CCGCTTGGAA 1620 TAAGGCCGGT GTGCGTTTGT CTATATGTTA TTTTCCACCA TATTGCCGTC TTTTGGCAAT 1680 GTGAGGGCCC GGAAACCTGG CCCTGTCTTC TTGACGAGCA TTCCTAGGGG TCTTTCCCCT 1740 CTCGCCAAAG GAATGCAAGG TCTGTTGAAT GTCGTGAAGG AAGCAGTTCC TCTGGAAGCT 1800 TCTTGAAGAC AAACAACGTC TGTAGCGACC CTTTGCAGGC AGCGGAACCC CCCACCTGGC 1860 GACAGGTGCC TCTGCGGCCA AAAGCCACGT GTATAAGATA CACCTGCAAA GGCGGCACAA 1920 CCCCAGTGCC ACGTTGTGAG TTGGATAGTT GTGGAAAGAG TCAAATGGCT CTCCTCAAGC 1980 GTATTCAACA AGGGGCTGAA GGATGCCCAG AAGGTACCCC ATTGTATGGG ATCTGATCTG 2040 GGGCCTCGGT GCACATGCTT TACATGTGTT TAGTCGAGGT TAAAAAAACG TCTAGGCCCC 2100 CCGAACCACG GGGACGTGGT TTTCCTTTGA AAAACACGAT ACGGGATCCA CCGGTCGCCA 2160 CCATGGGTAA AGGAGAAGAA CTTTTCACAG GAGTTGTCCC AATTCTTGTT GAATTAGATG 2220 GTGATGTTAA TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG 2280 GAAAACTTAC CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGCCAACAC 2340 TTGTCACTAC TTTCACTTAT GGTGTTCAAT GCTTTTCAAG ATACCCAGAT CATATGAAAC 2400 GGCATGACTT TTTCAAGAGT GCCATGCCCG AAGGTTATGT ACAGGAAAGA ACTATATTTT 2460 TCAAAGATGA CGGGAACTAC AAGACACGTG CTGAAGTCAA GTTTGAAGGT GATACCCTTG 2520 TTAATAGAAT CGAGTTAAAA GGTATTGATT TTAAAGAAGA TGGAAACATT CTTGGACACA 2580 AATTGGAATA CAACTATAAC TCACACAATG TATACATCAT GGCAGACAAA CAAAAGAATG 2640 GAACCAAAGT TAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG 2700 ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT 2760 ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGTCC 2820 TTCTTGAGTT TGTAACAGCT GCTGGGATTA CACATGGCAT GGATGAACTA TACAAGTCCG 2880 GATCTAGATA ACTGTATCGA TGGATCCGAA GGCGGGGACA GCAGTGCAGT GGTGGACAGA 2940 AAGCAAGTGA TCTAGGCCAG CAGCCTCCCT AAAGGGACTT CAGCCCACAA AGCCAAACTT 3000 GTGGCTTTAA TACAAGCTCT GTAAATGGTA AAAAAAAAAA AGTCTACACG GACAGCAGGT 3060 ATGCTCTTGC CACTGTACAG AGCAATATAC AGACAAAGAG AACTGTTGAC ATCTGCAGAG 3120 AAAGACCTAA GATGCTGTGG CTAAAAGAAA TCAGATGGCA AATCTAACCG CCCAGGCATC 3180 CTAAAGAGCA ATGATCCTGA CAGTCTGAAG ACTATCAAGT TATAGACAAA TTAAGACTGG 3240 TAAAAAAAAC CCTGTATAAA ATAGTAAAAA CTGAAAAAAG AAAACTAGTC CTCTCATGAG 3300 AAGACAGACC TGACATCTAC TGAAAAATAG ACTTTACTGG AAAAAATATG TGTATGAATA 3360 CCTTCTAGTT TTTGTGAACG TTCTCAAGAT GGATAAAAGC TTTTCCTTGT AAAACGAGAC 3420 TGATCAGATA GTCATCAAGA AGATTGTTAA AGAAAATTTT CCAAGGTTCG GAGTGCCAAA 3480 AGCAATAGTG TCAGATAATG GTCCTGCCTT TGTTGCCCAG GTAAGTCAGG GTGTGGCCAA 3540 GTATTTAGAG GTCAAATGAA AATTCCATTG TGTGTACAGA CCTCAGAGCT CAGGAAAGAT 3600 AAAAAAGAAT AAATAAAACT CTAAACAGAC CTTGACAAAA TTAATCCTAG AGACTGGCAC 3660 AGACTTACTT GGTACTCCTT CCCCTTGCCC TATTTAGAAC TGAGAATACT CCCTCTTGAT 3720 TCGGTTTTAC TCTTTTTAAG ATCCTTTATG GGGCTCCTAT GCCATCACTG TCTTAAATGA 3780 TGTGTTTAAA CCTATGTTGT TATAATAATG ATCTATATGT TAAGTTAAAA GGCTTGCAGG 3840 TGGTGCAGAA AGAAGTCTGG TCACAACTGG CTACAGTGAA CAAGCTGGGT ACCCCAAGGA 3900 CATCTTACCA GTTCCAGCCA GAGATCTGAT CTACGATCCC CGGGTCGACC CGGGTCGACC 3960 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 4020 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 4080 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 4140 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 4200 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 4260 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTCACGC TGCCGCAAGC 4320 ACTCAGGGCG CAAGGGCTGC TAAAGGAAGC GGAACACGTA GAAAGCCAGT CCGCAGAAAC 4380 GGTGCTGACC CCGGATGAAT GTCAGCTACT GGGCTATCTG GACAAGGGAA AACGCAAGCG 4440 CAAAGAGAAA GCAGGTAGCT TGCAGTGGGC TTACATGGCG ATAGCTAGAC TGGGCGGTTT 4500 TATGGACAGC AAGCGAACCG GAATTGCCAG CTGGGGCGCC CTCTGGTAAG GTTGGGAAGC 4560 CCTGCAAAGT AAACTGGATG GCTTTCTTGC CGCCAAGGAT CTGATGGCGC AGGGGATCAA 4620 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG 4680 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA 4740 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG 4800 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT 4860 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA 4920 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC 4980 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG 5040 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG 5100 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG 5160 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG 5220 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT 5280 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG 5340 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC 5400 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT 5460 GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC 5520 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGGAA TTCGTAATCT 5580 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 5640 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 5700 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 5760 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 5820 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 5880 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 5940 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 6000 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 6060 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 6120 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCCGAGAT GCGCCGCCTC GAGTACACCT 6180 GCGTCATGCT GAGACCCTCA AGCCTCACTA AAAGGGTCCC TGCCTAGTTC TGTTTACTAA 6240 TCTGCCTTAT TCTGTTTTTG TTCCCATGTT AAAGATAGAG TAAATGCAGT ATTCTCCACA 6300 TAGAGATATA GACTTCTGAA ATTCTAAGAT TAGAATTATT TACAAGAAGA AGTGGGGAA 6359 6891 base pairs nucleic acid single linear DNA (genomic) 16 TGAAGAATAA AAAATTACTG GCCTCTTGTG AGAACATGAA CTTTCACCTC GGAGCCCACC 60 CCCTCCCATC TGGAAAACAT ACTTGAGAAA AACATTTTCT GGAACAACCA CAGAATGTTT 120 CAACAGGCCA GATGTATTGC CAAACACAGG ATATGACTCT TTGGTTGAGT AAATTTGTGG 180 TTGTTAAACT TCCCCTATTC CCTCCCCATT CCCCCTCCCA GTTTGTGGTT TTTTCCTTTA 240 AAAGCTTGTG AAAAATTTGA GTCGTCGTCG AGACTCCTCT ACCCTGTGCA AAGGTGTATG 300 AGTTTCGACC CCAGAGCTCT GTGTGCTTTC TGTTGCTGCT TTATTTCGAC CCCAGAGCTC 360 TGGTCTGTGT GCTTTCATGT CGCTGCTTTA TTAAATCTTA CCTTCTACAT TTTATGTATG 420 GTCTCAGTGT CTTCTTGGGT ACGCGGCTGT CCCGGGACTT GAGTGTCTGA GTGAGGGTCT 480 TCCCTCGAGG GTCTTTCATT TGGTACATGG GCCGGGAATT CGAGAATCTT TCATTTGGTG 540 CATTGGCCGG GAATTCGAAA ATCTTTCATT TGGTGCATTG GCCGGGAAAC AGCGCGACCA 600 CCCAGAGGTC CTAGACCCAC TTAGAGGTAA GATTCTTTGT TCTGTTTTGG TCTGATGTCT 660 GTGTTCTGAT GTCTGTGTTC TGTTTCTAAG TCTGGTGCGA TCGCAGTTTC AGTTTTGCGG 720 ACGCTCAGTG AGACCGCGCT CCGAGAGGGA GTGCGGGGTG GATAAGGATA GACGTGTCCA 780 GGTGTCCACC GTCCGTTCGC CCTGGGAGAC GTCCCAGGAG GAACAGGGGA GGATCAGGGA 840 CGCCTGGTGG ACCCCTTTGA AGGCCAAGAG ACCATTTGGG GTTGCGAGAT CGTGGGTTCG 900 AGTCCCACCT CGTGCCCAGT TGCGAGATCG TGGGTTCGAG TCCCACCTCG TGTTTTGTTG 960 CGAGATCGTG GGTTCGAGTC CCACCTCGCG TCTGGTCACG GGATCGTGGG TTCGAGTCCC 1020 ACCTCGTGTT TTGTTGCGAG ATCGTGGGTT CGAGTCCCAC CTCGCGTCTG GTCACGGGAT 1080 CGTGGGTTCG AGTCCCACCT CGTGCAGAGG GTCTCAATTG GCCGGCCTTA GAGAGGCCAT 1140 CTGATTCTTC TGGTTTCTCT TTTTGTCTTA GTCTCGTGTC CGCTCTTGTT GTGACTACTG 1200 TTTTTCTAAA AATGGGACAA TCTGTGTCCA CTCCCCTTTC TCTGACTCTG GTTCTGTCGC 1260 TTGGTAATTT TGTTTGTTTA CGTTTGTTTT TGTGAGTCGT CTATGTTGTC TGTTACTATC 1320 TTGTTTTTGT TTGTGGTTTA CGGTTTCTGT GTGTGTCTTG TGTGTCTCTT TGTGTTCAGA 1380 CTTGGACTGA TGACTGACGA CTGTTTTTAA GTTATGCCTT CTAAAATAAG CCTAAAAATC 1440 CTGTCAGATC CCTATGCTGA CCACTTCCTT TCAGATCAAC AGCTGCCCTT ACTCGAGCTC 1500 AAGCTTCGAA TTCTGCAGTC GACGGTACCG CGGGGATCAA TTCCGCCCCC CCCCTAACGT 1560 TACTGGCCGA AGCCGCTTGG AATAAGGCCG GTGTGCGTTT GTCTATATGT TATTTTCCAC 1620 CATATTGCCG TCTTTTGGCA ATGTGAGGGC CCGGAAACCT GGCCCTGTCT TCTTGACGAG 1680 CATTCCTAGG GGTCTTTCCC CTCTCGCCAA AGGAATGCAA GGTCTGTTGA ATGTCGTGAA 1740 GGAAGCAGTT CCTCTGGAAG CTTCTTGAAG ACAAACAACG TCTGTAGCGA CCCTTTGCAG 1800 GCAGCGGAAC CCCCCACCTG GCGACAGGTG CCTCTGCGGC CAAAAGCCAC GTGTATAAGA 1860 TACACCTGCA AAGGCGGCAC AACCCCAGTG CCACGTTGTG AGTTGGATAG TTGTGGAAAG 1920 AGTCAAATGG CTCTCCTCAA GCGTATTCAA CAAGGGGCTG AAGGATGCCC AGAAGGTACC 1980 CCATTGTATG GGATCTGATC TGGGGCCTCG GTGCACATGC TTTACATGTG TTTAGTCGAG 2040 GTTAAAAAAC GTCTAGGCCC CCCGAACCAC GGGGACGTGG TTTTCCTTTG AAAAACACGA 2100 GCGGGATCAA TTCCGCCCCC CCCCTAACGT TACTGGCCGA AGCCGCTTGG AATAAGGCCG 2160 GTGTGCGTTT GTCTATATGT TATTTTCCAC CATATTGCCG TCTTTTGGCA ATGTGAGGGC 2220 CCGGAAACCT GGCCCTGTCT TCTTGACGAG CATTCCTAGG GGTCTTTCCC CTCTCGCCAA 2280 AGGAATGCAA GGTCTGTTGA ATGTCGTGAA GGAAGCAGTT CCTCTGGAAG CTTCTTGAAG 2340 ACAAACAACG TCTGTAGCGA CCCTTTGCAG GCAGCGGAAC CCCCCACCTG GCGACAGGTG 2400 CCTCTGCGGC CAAAAGCCAC GTGTATAAGA TACACCTGCA AAGGCGGCAC AACCCCAGTG 2460 CCACGTTGTG AGTTGGATAG TTGTGGAAAG AGTCAAATGG CTCTCCTCAA GCGTATTCAA 2520 CAAGGGGCTG AAGGATGCCC AGAAGGTACC CCATTGTATG GGATCTGATC TGGGGCCTCG 2580 GTGCACATGC TTTACATGTG TTTAGTCGAG GTTAAAAAAA CGTCTAGGCC CCCCGAACCA 2640 CGGGGACGTG GTTTTCCTTT GAAAAACACG ATACGGGATC CACCGGTCGC CACCATGGGT 2700 AAAGGAGAAG AACTTTTCAC AGGAGTTGTC CCAATTCTTG TTGAATTAGA TGGTGATGTT 2760 AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG ATGCAACATA CGGAAAACTT 2820 ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC CATGGCCAAC ACTTGTCACT 2880 ACTTTCACTT ATGGTGTTCA ATGCTTTTCA AGATACCCAG ATCATATGAA ACGGCATGAC 2940 TTTTTCAAGA GTGCCATGCC CGAAGGTTAT GTACAGGAAA GAACTATATT TTTCAAAGAT 3000 GACGGGAACT ACAAGACACG TGCTGAAGTC AAGTTTGAAG GTGATACCCT TGTTAATAGA 3060 ATCGAGTTAA AAGGTATTGA TTTTAAAGAA GATGGAAACA TTCTTGGACA CAAATTGGAA 3120 TACAACTATA ACTCACACAA TGTATACATC ATGGCAGACA AACAAAAGAA TGGAACCAAA 3180 GTTAACTTCA AAATTAGACA CAACATTGAA GATGGAAGCG TTCAACTAGC AGACCATTAT 3240 CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC CAGACAACCA TTACCTGTCC 3300 ACACAATCTG CCCTTTCGAA AGATCCCAAC GAAAAGAGAG ACCACATGGT CCTTCTTGAG 3360 TTTGTAACAG CTGCTGGGAT TACACATGGC ATGGATGAAC TATACAAGTC CGGATCTAGA 3420 TAACTGTATC GATGGATCCG AAGGCGGGGA CAGCAGTGCA GTGGTGGACA GAAAGCAAGT 3480 GATCTAGGCC AGCAGCCTCC CTAAAGGGAC TTCAGCCCAC AAAGCCAAAC TTGTGGCTTT 3540 AATACAAGCT CTGTAAATGG TAAAAAAAAA AAAGTCTACA CGGACAGCAG GTATGCTCTT 3600 GCCACTGTAC AGAGCAATAT ACAGACAAAG AGAACTGTTG ACATCTGCAG AGAAAGACCT 3660 AAGATGCTGT GGCTAAAAGA AATCAGATGG CAAATCTAAC CGCCCAGGCA TCCTAAAGAG 3720 CAATGATCCT GACAGTCTGA AGACTATCAA GTTATAGACA AATTAAGACT GGTAAAAAAA 3780 ACCCTGTATA AAATAGTAAA AACTGAAAAA AGAAAACTAG TCCTCTCATG AGAAGACAGA 3840 CCTGACATCT ACTGAAAAAT AGACTTTACT GGAAAAAATA TGTGTATGAA TACCTTCTAG 3900 TTTTTGTGAA CGTTCTCAAG ATGGATAAAA GCTTTTCCTT GTAAAACGAG ACTGATCAGA 3960 TAGTCATCAA GAAGATTGTT AAAGAAAATT TTCCAAGGTT CGGAGTGCCA AAAGCAATAG 4020 TGTCAGATAA TGGTCCTGCC TTTGTTGCCC AGGTAAGTCA GGGTGTGGCC AAGTATTTAG 4080 AGGTCAAATG AAAATTCCAT TGTGTGTACA GACCTCAGAG CTCAGGAAAG ATAAAAAAGA 4140 ATAAATAAAA CTCTAAACAG ACCTTGACAA AATTAATCCT AGAGACTGGC ACAGACTTAC 4200 TTGGTACTCC TTCCCCTTGC CCTATTTAGA ACTGAGAATA CTCCCTCTTG ATTCGGTTTT 4260 ACTCTTTTTA AGATCCTTTA TGGGGCTCCT ATGCCATCAC TGTCTTAAAT GATGTGTTTA 4320 AACCTATGTT GTTATAATAA TGATCTATAT GTTAAGTTAA AAGGCTTGCA GGTGGTGCAG 4380 AAAGAAGTCT GGTCACAACT GGCTACAGTG AACAAGCTGG GTACCCCAAG GACATCTTAC 4440 CAGTTCCAGC CAGAGATCTG ATCTACGATC CCCGGGTCGA CCCGGGTCGA CCCTGTGGAA 4500 TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG 4560 CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG 4620 AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC 4680 CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT 4740 TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG 4800 AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTTCAC GCTGCCGCAA GCACTCAGGG 4860 CGCAAGGGCT GCTAAAGGAA GCGGAACACG TAGAAAGCCA GTCCGCAGAA ACGGTGCTGA 4920 CCCCGGATGA ATGTCAGCTA CTGGGCTATC TGGACAAGGG AAAACGCAAG CGCAAAGAGA 4980 AAGCAGGTAG CTTGCAGTGG GCTTACATGG CGATAGCTAG ACTGGGCGGT TTTATGGACA 5040 GCAAGCGAAC CGGAATTGCC AGCTGGGGCG CCCTCTGGTA AGGTTGGGAA GCCCTGCAAA 5100 GTAAACTGGA TGGCTTTCTT GCCGCCAAGG ATCTGATGGC GCAGGGGATC AAGATCTGAT 5160 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTGC 5220 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 5280 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC 5340 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC 5400 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 5460 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 5520 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 5580 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT 5640 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC 5700 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 5760 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 5820 CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG 5880 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG 5940 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG 6000 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT 6060 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGG AATTCGTAAT CTGCTGCTTG 6120 CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT 6180 CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG 6240 TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG 6300 CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC 6360 TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA 6420 CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCATTGA 6480 GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC 6540 GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT 6600 GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG 6660 AGCCTATGGA AAAACGCCAG CAACGCCGAG ATGCGCCGCC TCGAGTACAC CTGCGTCATG 6720 CTGAGACCCT CAAGCCTCAC TAAAAGGGTC CCTGCCTAGT TCTGTTTACT AATCTGCCTT 6780 ATTCTGTTTT TGTTCCCATG TTAAAGATAG AGTAAATGCA GTATTCTCCA CATAGAGATA 6840 TAGACTTCTG AAATTCTAAG ATTAGAATTA TTTACAAGAA GAAGTGGGGA A 6891 6321 base pairs nucleic acid single linear DNA (genomic) 17 TGAAGAATAA AAAATTACTG GCCTCTTGTG AGAACATGAA CTTTCACCTC GGAGCCCACC 60 CCCTCCCATC TGGAAAACAT ACTTGAGAAA AACATTTTCT GGAACAACCA CAGAATGTTT 120 CAACAGGCCA GATGTATTGC CAAACACAGG ATATGACTCT TTGGTTGAGT AAATTTGTGG 180 TTGTTAAACT TCCCCTATTC CCTCCCCATT CCCCCTCCCA GTTTGTGGTT TTTTCCTTTA 240 AAAGCTTGTG AAAAATTTGA GTCGTCGTCG AGACTCCTCT ACCCTGTGCA AAGGTGTATG 300 AGTTTCGACC CCAGAGCTCT GTGTGCTTTC TGTTGCTGCT TTATTTCGAC CCCAGAGCTC 360 TGGTCTGTGT GCTTTCATGT CGCTGCTTTA TTAAATCTTA CCTTCTACAT TTTATGTATG 420 GTCTCAGTGT CTTCTTGGGT ACGCGGCTGT CCCGGGACTT GAGTGTCTGA GTGAGGGTCT 480 TCCCTCGAGG GTCTTTCATT TGGTACATGG GCCGGGAATT CGAGAATCTT TCATTTGGTG 540 CATTGGCCGG GAATTCGAAA ATCTTTCATT TGGTGCATTG GCCGGGAAAC AGCGCGACCA 600 CCCAGAGGTC CTAGACCCAC TTAGAGGTAA GATTCTTTGT TCTGTTTTGG TCTGATGTCT 660 GTGTTCTGAT GTCTGTGTTC TGTTTCTAAG TCTGGTGCGA TCGCAGTTTC AGTTTTGCGG 720 ACGCTCAGTG AGACCGCGCT CCGAGAGGGA GTGCGGGGTG GATAAGGATA GACGTGTCCA 780 GGTGTCCACC GTCCGTTCGC CCTGGGAGAC GTCCCAGGAG GAACAGGGGA GGATCAGGGA 840 CGCCTGGTGG ACCCCTTTGA AGGCCAAGAG ACCATTTGGG GTTGCGAGAT CGTGGGTTCG 900 AGTCCCACCT CGTGCCCAGT TGCGAGATCG TGGGTTCGAG TCCCACCTCG TGTTTTGTTG 960 CGAGATCGTG GGTTCGAGTC CCACCTCGCG TCTGGTCACG GGATCGTGGG TTCGAGTCCC 1020 ACCTCGTGTT TTGTTGCGAG ATCGTGGGTT CGAGTCCCAC CTCGCGTCTG GTCACGGGAT 1080 CGTGGGTTCG AGTCCCACCT CGTGCAGAGG GTCTCAATTG GCCGGCCTTA GAGAGGCCAT 1140 CTGATTCTTC TGGTTTCTCT TTTTGTCTTA GTCTCGTGTC CGCTCTTGTT GTGACTACTG 1200 TTTTTCTAAA AATGGGACAA TCTGTGTCCA CTCCCCTTTC TCTGACTCTG GTTCTGTCGC 1260 TTGGTAATTT TGTTTGTTTA CGTTTGTTTT TGTGAGTCGT CTATGTTGTC TGTTACTATC 1320 TTGTTTTTGT TTGTGGTTTA CGGTTTCTGT GTGTGTCTTG TGTGTCTCTT TGTGTTCAGA 1380 CTTGGACTGA TGACTGACGA CTGTTTTTAA GTTATGCCTT CTAAAATAAG CCTAAAAATC 1440 CTGTCAGATC CCTATGCTGA CCACTTCCTT TCAGATCAAC AGCTGCCCTT ACTCGAGCTC 1500 AAGCTTCGAA TTCTGCAGTC GACGGTACCG CGGGGATCAA TTCCGCCCCC CCCCTAACGT 1560 TACTGGCCGA AGCCGCTTGG AATAAGGCCG GTGTGCGTTT GTCTATATGT TATTTTCCAC 1620 CATATTGCCG TCTTTTGGCA ATGTGAGGGC CCGGAAACCT GGCCCTGTCT TCTTGACGAG 1680 CATTCCTAGG GGTCTTTCCC CTCTCGCCAA AGGAATGCAA GGTCTGTTGA ATGTCGTGAA 1740 GGAAGCAGTT CCTCTGGAAG CTTCTTGAAG ACAAACAACG TCTGTAGCGA CCCTTTGCAG 1800 GCAGCGGAAC CCCCCACCTG GCGACAGGTG CCTCTGCGGC CAAAAGCCAC GTGTATAAGA 1860 TACACCTGCA AAGGCGGCAC AACCCCAGTG CCACGTTGTG AGTTGGATAG TTGTGGAAAG 1920 AGTCAAATGG CTCTCCTCAA GCGTATTCAA CAAGGGGCTG AAGGATGCCC AGAAGGTACC 1980 CCATTGTATG GGATCTGATC TGGGGCCTCG GTGCACATGC TTTACATGTG TTTAGTCGAG 2040 GTTAAAAAAA CGTCTAGGCC CCCCGAACCA CGGGGACGTG GTTTTCCTTT GAAAAACACG 2100 ATACGGGATC CACCGGTCGC CACCATGGGT AAAGGAGAAG AACTTTTCAC AGGAGTTGTC 2160 CCAATTCTTG TTGAATTAGA TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG 2220 GGTGAAGGTG ATGCAACATA CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA 2280 CTACCTGTTC CATGGCCAAC ACTTGTCACT ACTTTCACTT ATGGTGTTCA ATGCTTTTCA 2340 AGATACCCAG ATCATATGAA ACGGCATGAC TTTTTCAAGA GTGCCATGCC CGAAGGTTAT 2400 GTACAGGAAA GAACTATATT TTTCAAAGAT GACGGGAACT ACAAGACACG TGCTGAAGTC 2460 AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA TTTTAAAGAA 2520 GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA ACTCACACAA TGTATACATC 2580 ATGGCAGACA AACAAAAGAA TGGAACCAAA GTTAACTTCA AAATTAGACA CAACATTGAA 2640 GATGGAAGCG TTCAACTAGC AGACCATTAT CAACAAAATA CTCCAATTGG CGATGGCCCT 2700 GTCCTTTTAC CAGACAACCA TTACCTGTCC ACACAATCTG CCCTTTCGAA AGATCCCAAC 2760 GAAAAGAGAG ACCACATGGT CCTTCTTGAG TTTGTAACAG CTGCTGGGAT TACACATGGC 2820 ATGGATGAAC TATACAAGTC CGGATCTAGA TAACTGTATC GATGGATCCG AAGGCGGGGA 2880 CAGCAGTGCA GTGGTGGACA GAAAGCAAGT GATCTAGGCC AGCAGCCTCC CTAAAGGGAC 2940 TTCAGCCCAC AAAGCCAAAC TTGTGGCTTT AATACAAGCT CTGTAAATGG TAAAAAAAAA 3000 AAAGTCTACA CGGACAGCAG GTATGCTCTT GCCACTGTAC AGAGCAATAT ACAGACAAAG 3060 AGAACTGTTG ACATCTGCAG AGAAAGACCT AAGATGCTGT GGCTAAAAGA AATCAGATGG 3120 CAAATCTAAC CGCCCAGGCA TCCTAAAGAG CAATGATCCT GACAGTCTGA AGACTATCAA 3180 GTTATAGACA AATTAAGACT GGTAAAAAAA ACCCTGTATA AAATAGTAAA AACTGAAAAA 3240 AGAAAACTAG TCCTCTCATG AGAAGACAGA CCTGACATCT ACTGAAAAAT AGACTTTACT 3300 GGAAAAAATA TGTGTATGAA TACCTTCTAG TTTTTGTGAA CGTTCTCAAG ATGGATAATT 3360 GCTTTTCCTT GTAAAACGAG ACTGATCAGA TAGTCATCAA GAAGATTGTT AAAGAAAACC 3420 TTCCAAGGTT CGGAGTGCCA AAAGCAATAG TGTCAGATAA TGGTCCTGCC TTTGTTGCCC 3480 AGGTAAGTCA GGGTGTGGCC AAGTATTTAG AGGTCAAATG AAAATTCCAT TGTGTGTACA 3540 GACCTCAGAG CTCAGGAAAG ATAAAAAAGA ATAAATAAAA CTCTAAACAG ACCTTGACAA 3600 AATTAATCCT AGAGACTGGC ACAGACTTAC TTGGTACTCC TTCCCCTTGC CCTATTTAGA 3660 ACTGAGAATA CTCCCTCTTG ATTCGGTTTT ACTCTTTTTA AGATCCTTTA TGGGGCTCCT 3720 ATGCCATCAC TGTCTTAAAT GATGTGTTTA AACCTATGTT GTTATAATAA TGATCTATAT 3780 GTTAAGTTAA AAGGCTTGCA GGTGGTGCAG AAAGAAGTCT GGTCACAACT GGCTACAGTG 3840 AACAAGCTGG GTACCCCAAG GACATCTTAC CAGTTCCAGC CAGAGATCTG ATCTACGATC 3900 CCCGGGTCGA CCCGGGTCGA CCCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC 3960 AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG 4020 TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC 4080 AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC 4140 CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC 4200 GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA 4260 AAAGCTTCAC GCTGCCGCAA GCACTCAGGG CGCAAGGGCT GCTAAAGGAA GCGGAACACG 4320 TAGAAAGCCA GTCCGCAGAA ACGGTGCTGA CCCCGGATGA ATGTCAGCTA CTGGGCTATC 4380 TGGACAAGGG AAAACGCAAG CGCAAAGAGA AAGCAGGTAG CTTGCAGTGG GCTTACATGG 4440 CGATAGCTAG ACTGGGCGGT TTTATGGACA GCAAGCGAAC CGGAATTGCC AGCTGGGGCG 4500 CCCTCTGGTA AGGTTGGGAA GCCCTGCAAA GTAAACTGGA TGGCTTTCTT GCCGCCAAGG 4560 ATCTGATGGC GCAGGGGATC AAGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 4620 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 4680 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 4740 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 4800 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 4860 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 4920 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 4980 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 5040 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 5100 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 5160 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 5220 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA 5280 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 5340 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 5400 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC 5460 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 5520 TCCGGGACGG AATTCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG 5580 GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA 5640 GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC 5700 TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT 5760 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG 5820 CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC 5880 GAACTGAGAT ACCTACAGCG TGAGCATTGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG 5940 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA 6000 GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT 6060 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCCGAG 6120 ATGCGCCGCC TCGAGTACAC CTGCGTCATG CTGAGACCCT CAAGCCTCAC TAAAAGGGTC 6180 CCTGCCTAGT TCTGTTTACT AATCTGCCTT ATTCTGTTTT TGTTCCCATG TTAAAGATAG 6240 AGTAAATGCA GTATTCTCCA CATAGAGATA TAGACTTCTG AAATTCTAAG ATTAGAATTA 6300 TTTACAAGAA GAAGTGGGGA A 6321 5754 base pairs nucleic acid single linear DNA (genomic) 18 TGAAGAATAA AAAATTACTG GCCTCTTGTG AGAACATGAA CTTTCACCTC GGAGCCCACC 60 CCCTCCCATC TGGAAAACAT ACTTGAGAAA AACATTTTCT GGAACAACCA CAGAATGTTT 120 CAACAGGCCA GATGTATTGC CAAACACAGG ATATGACTCT TTGGTTGAGT AAATTTGTGG 180 TTGTTAAACT TCCCCTATTC CCTCCCCATT CCCCCTCCCA GTTTGTGGTT TTTTCCTTTA 240 AAAGCTTGTG AAAAATTTGA GTCGTCGTCG AGACTCCTCT ACCCTGTGCA AAGGTGTATG 300 AGTTTCGACC CCAGAGCTCT GTGTGCTTTC TGTTGCTGCT TTATTTCGAC CCCAGAGCTC 360 TGGTCTGTGT GCTTTCATGT CGCTGCTTTA TTAAATCTTA CCTTCTACAT TTTATGTATG 420 GTCTCAGTGT CTTCTTGGGT ACGCGGCTGT CCCGGGACTT GAGTGTCTGA GTGAGGGTCT 480 TCCCTCGAGG GTCTTTCATT TGGTACATGG GCCGGGAATT CGAGAATCTT TCATTTGGTG 540 CATTGGCCGG GAATTCGAAA ATCTTTCATT TGGTGCATTG GCCGGGAAAC AGCGCGACCA 600 CCCAGAGGTC CTAGACCCAC TTAGAGGTAA GATTCTTTGT TCTGTTTTGG TCTGATGTCT 660 GTGTTCTGAT GTCTGTGTTC TGTTTCTAAG TCTGGTGCGA TCGCAGTTTC AGTTTTGCGG 720 ACGCTCAGTG AGACCGCGCT CCGAGAGGGA GTGCGGGGTG GATAAGGATA GACGTGTCCA 780 GGTGTCCACC GTCCGTTCGC CCTGGGAGAC GTCCCAGGAG GAACAGGGGA GGATCAGGGA 840 CGCCTGGTGG ACCCCTTTGA AGGCCAAGAG ACCATTTGGG GTTGCGAGAT CGTGGGTTCG 900 AGTCCCACCT CGTGCCCAGT TGCGAGATCG TGGGTTCGAG TCCCACCTCG TGTTTTGTTG 960 CGAGATCGTG GGTTCGAGTC CCACCTCGCG TCTGGTCACG GGATCGTGGG TTCGAGTCCC 1020 ACCTCGTGTT TTGTTGCGAG ATCGTGGGTT CGAGTCCCAC CTCGCGTCTG GTCACGGGAT 1080 CGTGGGTTCG AGTCCCACCT CGTGCAGAGG GTCTCAATTG GCCGGCCTTA GAGAGGCCAT 1140 CTGATTCTTC TGGTTTCTCT TTTTGTCTTA GTCTCGTGTC CGCTCTTGTT GTGACTACTG 1200 TTTTTCTAAA AATGGGACAA TCTGTGTCCA CTCCCCTTTC TCTGACTCTG GTTCTGTCGC 1260 TTGGTAATTT TGTTTGTTTA CGTTTGTTTT TGTGAGTCGT CTATGTTGTC TGTTACTATC 1320 TTGTTTTTGT TTGTGGTTTA CGGTTTCTGT GTGTGTCTTG TGTGTCTCTT TGTGTTCAGA 1380 CTTGGACTGA TGACTGACGA CTGTTTTTAA GTTATGCCTT CTAAAATAAG CCTAAAAATC 1440 CTGTCAGATC CCTATGCTGA CCACTTCCTT TCAGATCAAC AGCTGCCCTT ACTCGAGCTC 1500 AAGCTTCGAA TTCTGCAGTC GACGGTACCG CGGGCCCGGG ATCCACCGGT CGCCACCATG 1560 GGTAAAGGAG AAGAACTTTT CACAGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT 1620 GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA 1680 CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGCC AACACTTGTC 1740 ACTACTTTCA CTTATGGTGT TCAATGCTTT TCAAGATACC CAGATCATAT GAAACGGCAT 1800 GACTTTTTCA AGAGTGCCAT GCCCGAAGGT TATGTACAGG AAAGAACTAT ATTTTTCAAA 1860 GATGACGGGA ACTACAAGAC ACGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT 1920 AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG 1980 GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAACC 2040 AAAGTTAACT TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT 2100 TATCAACAAA ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG 2160 TCCACACAAT CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT GGTCCTTCTT 2220 GAGTTTGTAA CAGCTGCTGG GATTACACAT GGCATGGATG AACTATACAA GTCCGGATCT 2280 AGATAACTGT ATCGATGGAT CCGAAGGCGG GGACAGCAGT GCAGTGGTGG ACAGAAAGCA 2340 AGTGATCTAG GCCAGCAGCC TCCCTAAAGG GACTTCAGCC CACAAAGCCA AACTTGTGGC 2400 TTTAATACAA GCTCTGTAAA TGGTAAAAAA AAAAAAGTCT ACACGGACAG CAGGTATGCT 2460 CTTGCCACTG TACAGAGCAA TATACAGACA AAGAGAACTG TTGACATCTG CAGAGAAAGA 2520 CCTAAGATGC TGTGGCTAAA AGAAATCAGA TGGCAAATCT AACCGCCCAG GCATCCTAAA 2580 GAGCAATGAT CCTGACAGTC TGAAGACTAT CAAGTTATAG ACAAATTAAG ACTGGTAAAA 2640 AAAACCCTGT ATAAAATAGT AAAAACTGAA AAAAGAAAAC TAGTCCTCTC ATGAGAAGAC 2700 AGACCTGACA TCTACTGAAA AATAGACTTT ACTGGAAAAA ATATGTGTAT GAATACCTTC 2760 TAGTTTTTGT GAACGTTCTC AAGATGGATA AAAGCTTTTC CTTGTAAAAC GAGACTGATC 2820 AGATAGTCAT CAAGAAGATT GTTAAAGAAA ATTTTCCAAG GTTCGGAGTG CCAAAAGCAA 2880 TAGTGTCAGA TAATGGTCCT GCCTTTGTTG CCCAGGTAAG TCAGGGTGTG GCCAAGTATT 2940 TAGAGGTCAA ATGAAAATTC CATTGTGTGT ACAGACCTCA GAGCTCAGGA AAGATAAAAA 3000 AGAATAAATA AAACTCTAAA CAGACCTTGA CAAAATTAAT CCTAGAGACT GGCACAGACT 3060 TACTTGGTAC TCCTTCCCCT TGCCCTATTT AGAACTGAGA ATACTCCCTC TTGATTCGGT 3120 TTTACTCTTT TTAAGATCCT TTATGGGGCT CCTATGCCAT CACTGTCTTA AATGATGTGT 3180 TTAAACCTAT GTTGTTATAA TAATGATCTA TATGTTAAGT TAAAAGGCTT GCAGGTGGTG 3240 CAGAAAGAAG TCTGGTCACA ACTGGCTACA GTGAACAAGC TGGGTACCCC AAGGACATCT 3300 TACCAGTTCC AGCCAGAGAT CTGATCTACG ATCCCCGGGT CGACCCGGGT CGACCCTGTG 3360 GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA 3420 AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG 3480 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC 3540 GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT 3600 TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG 3660 AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTT CACGCTGCCG CAAGCACTCA 3720 GGGCGCAAGG GCTGCTAAAG GAAGCGGAAC ACGTAGAAAG CCAGTCCGCA GAAACGGTGC 3780 TGACCCCGGA TGAATGTCAG CTACTGGGCT ATCTGGACAA GGGAAAACGC AAGCGCAAAG 3840 AGAAAGCAGG TAGCTTGCAG TGGGCTTACA TGGCGATAGC TAGACTGGGC GGTTTTATGG 3900 ACAGCAAGCG AACCGGAATT GCCAGCTGGG GCGCCCTCTG GTAAGGTTGG GAAGCCCTGC 3960 AAAGTAAACT GGATGGCTTT CTTGCCGCCA AGGATCTGAT GGCGCAGGGG ATCAAGATCT 4020 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 4080 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 4140 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 4200 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 4260 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 4320 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 4380 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 4440 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 4500 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 4560 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 4620 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 4680 CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 4740 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 4800 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 4860 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 4920 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGGAATTCGT AATCTGCTGC 4980 TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA 5040 ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA 5100 GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT 5160 CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG 5220 GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC 5280 ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT 5340 TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG 5400 GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT 5460 CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG 5520 CGGAGCCTAT GGAAAAACGC CAGCAACGCC GAGATGCGCC GCCTCGAGTA CACCTGCGTC 5580 ATGCTGAGAC CCTCAAGCCT CACTAAAAGG GTCCCTGCCT AGTTCTGTTT ACTAATCTGC 5640 CTTATTCTGT TTTTGTTCCC ATGTTAAAGA TAGAGTAAAT GCAGTATTCT CCACATAGAG 5700 ATATAGACTT CTGAAATTCT AAGATTAGAA TTATTTACAA GAAGAAGTGG GGAA 5754 5754 base pairs nucleic acid single linear DNA (genomic) 19 TGAAGAATAA AAAATTACTG GCCTCTTGTG AGAACATGAA CTTTCACCTC GGAGCCCACC 60 CCCTCCCATC TGGAAAACAT ACTTGAGAAA AACATTTTCT GGAACAACCA CAGAATGTTT 120 CAACAGGCCA GATGTATTGC CAAACACAGG ATATGACTCT TTGGTTGAGT AAATTTGTGG 180 TTGTTAAACT TCCCCTATTC CCTCCCCATT CCCCCTCCCA GTTTGTGGTT TTTTCCTTTA 240 AAAGCTTGTG AAAAATTTGA GTCGTCGTCG AGACTCCTCT ACCCTGTGCA AAGGTGTATG 300 AGTTTCGACC CCAGAGCTCT GTGTGCTTTC TGTTGCTGCT TTATTTCGAC CCCAGAGCTC 360 TGGTCTGTGT GCTTTCATGT CGCTGCTTTA TTAAATCTTA CCTTCTACAT TTTATGTATG 420 GTCTCAGTGT CTTCTTGGGT ACGCGGCTGT CCCGGGACTT GAGTGTCTGA GTGAGGGTCT 480 TCCCTCGAGG GTCTTTCATT TGGTACATGG GCCGGGAATT CGAGAATCTT TCATTTGGTG 540 CATTGGCCGG GAATTCGAAA ATCTTTCATT TGGTGCATTG GCCGGGAAAC AGCGCGACCA 600 CCCAGAGGTC CTAGACCCAC TTAGAGGTAA GATTCTTTGT TCTGTTTTGG TCTGATGTCT 660 GTGTTCTGAT GTCTGTGTTC TGTTTCTAAG TCTGGTGCGA TCGCAGTTTC AGTTTTGCGG 720 ACGCTCAGTG AGACCGCGCT CCGAGAGGGA GTGCGGGGTG GATAAGGATA GACGTGTCCA 780 GGTGTCCACC GTCCGTTCGC CCTGGGAGAC GTCCCAGGAG GAACAGGGGA GGATCAGGGA 840 CGCCTGGTGG ACCCCTTTGA AGGCCAAGAG ACCATTTGGG GTTGCGAGAT CGTGGGTTCG 900 AGTCCCACCT CGTGCCCAGT TGCGAGATCG TGGGTTCGAG TCCCACCTCG TGTTTTGTTG 960 CGAGATCGTG GGTTCGAGTC CCACCTCGCG TCTGGTCACG GGATCGTGGG TTCGAGTCCC 1020 ACCTCGTGTT TTGTTGCGAG ATCGTGGGTT CGAGTCCCAC CTCGCGTCTG GTCACGGGAT 1080 CGTGGGTTCG AGTCCCACCT CGTGCAGAGG GTCTCAATTG GCCGGCCTTA GAGAGGCCAT 1140 CTGATTCTTC TGGTTTCTCT TTTTGTCTTA GTCTCGTGTC CGCTCTTGTT GTGACTACTG 1200 TTTTTCTAAA AATGGGACAA TCTGTGTCCA CTCCCCTTTC TCTGACTCTG GTTCTGTCGC 1260 TTGGTAATTT TGTTTGTTTA CGTTTGTTTT TGTGAGTCGT CTATGTTGTC TGTTACTATC 1320 TTGTTTTTGT TTGTGGTTTA CGGTTTCTGT GTGTGTCTTG TGTGTCTCTT TGTGTTCAGA 1380 CTTGGACTGA TGACTGACGA CTGTTTTTAA GTTATGCCTT CTAAAATAAG CCTAAAAATC 1440 CTGTCAGATC CCTATGCTGA CCACTTCCTT TCAGATCAAC AGCTGCCCTT ACTCGAGCTC 1500 AAGCTTCGAA TTCTGCAGTC GACGGTACCG CGGGCCCGGG ATCCACCGGT CGCCACCATG 1560 GGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT 1620 GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA 1680 CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGCC AACACTTGTC 1740 ACTACTTTCT CTTATGGTGT TCAATGCTTT TCAAGATACC CAGATCATAT GAAACGGCAT 1800 GACTTTTTCA AGAGTGCCAT GCCCGAAGGT TATGTACAGG AAAGAACTAT ATTTTTCAAA 1860 GATGACGGGA ACTACAAGAC ACGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT 1920 AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG 1980 GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAACC 2040 AAAGTTAACT TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT 2100 TATCAACAAA ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG 2160 TCCACACAAT CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT GGTCCTTCTT 2220 GAGTTTGTAA CAGCTGCTGG GATTACACAT GGCATGGATG AACTATACAA GTCCGGATCT 2280 AGATAACTGT ATCGATGGAT CCGAAGGCGG GGACAGCAGT GCAGTGGTGG ACAGAAAGCA 2340 AGTGATCTAG GCCAGCAGCC TCCCTAAAGG GACTTCAGCC CACAAAGCCA AACTTGTGGC 2400 TTTAATACAA GCTCTGTAAA TGGTAAAAAA AAAAAAGTCT ACACGGACAG CAGGTATGCT 2460 CTTGCCACTG TACAGAGCAA TATACAGACA AAGAGAACTG TTGACATCTG CAGAGAAAGA 2520 CCTAAGATGC TGTGGCTAAA AGAAATCAGA TGGCAAATCT AACCGCCCAG GCATCCTAAA 2580 GAGCAATGAT CCTGACAGTC TGAAGACTAT CAAGTTATAG ACAAATTAAG ACTGGTAAAA 2640 AAAACCCTGT ATAAAATAGT AAAAACTGAA AAAAGAAAAC TAGTCCTCTC ATGAGAAGAC 2700 AGACCTGACA TCTACTGAAA AATAGACTTT ACTGGAAAAA ATATGTGTAT GAATACCTTC 2760 TAGTTTTTGT GAACGTTCTC AAGATGGATA AAAGCTTTTC CTTGTAAAAC GAGACTGATC 2820 AGATAGTCAT CAAGAAGATT GTTAAAGAAA ATTTTCCAAG GTTCGGAGTG CCAAAAGCAA 2880 TAGTGTCAGA TAATGGTCCT GCCTTTGTTG CCCAGGTAAG TCAGGGTGTG GCCAAGTATT 2940 TAGAGGTCAA ATGAAAATTC CATTGTGTGT ACAGACCTCA GAGCTCAGGA AAGATAAAAA 3000 AGAATAAATA AAACTCTAAA CAGACCTTGA CAAAATTAAT CCTAGAGACT GGCACAGACT 3060 TACTTGGTAC TCCTTCCCCT TGCCCTATTT AGAACTGAGA ATACTCCCTC TTGATTCGGT 3120 TTTACTCTTT TTAAGATCCT TTATGGGGCT CCTATGCCAT CACTGTCTTA AATGATGTGT 3180 TTAAACCTAT GTTGTTATAA TAATGATCTA TATGTTAAGT TAAAAGGCTT GCAGGTGGTG 3240 CAGAAAGAAG TCTGGTCACA ACTGGCTACA GTGAACAAGC TGGGTACCCC AAGGACATCT 3300 TACCAGTTCC AGCCAGAGAT CTGATCTACG ATCCCCGGGT CGACCCGGGT CGACCCTGTG 3360 GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA 3420 AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG 3480 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC 3540 GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT 3600 TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG 3660 AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTT CACGCTGCCG CAAGCACTCA 3720 GGGCGCAAGG GCTGCTAAAG GAAGCGGAAC ACGTAGAAAG CCAGTCCGCA GAAACGGTGC 3780 TGACCCCGGA TGAATGTCAG CTACTGGGCT ATCTGGACAA GGGAAAACGC AAGCGCAAAG 3840 AGAAAGCAGG TAGCTTGCAG TGGGCTTACA TGGCGATAGC TAGACTGGGC GGTTTTATGG 3900 ACAGCAAGCG AACCGGAATT GCCAGCTGGG GCGCCCTCTG GTAAGGTTGG GAAGCCCTGC 3960 AAAGTAAACT GGATGGCTTT CTTGCCGCCA AGGATCTGAT GGCGCAGGGG ATCAAGATCT 4020 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 4080 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 4140 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 4200 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCAC 4260 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGCC 4320 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 4380 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 4440 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 4500 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 4560 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 4620 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 4680 CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 4740 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 4800 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 4860 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 4920 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGGAATTCGT AATCTGCTGC 4980 TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA 5040 ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA 5100 GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT 5160 CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG 5220 GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC 5280 ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT 5340 TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG 5400 GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT 5460 CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG 5520 CGGAGCCTAT GGAAAAACGC CAGCAACGCC GAGATGCGCC GCCTCGAGTA CACCTGCGTC 5580 ATGCTGAGAC CCTCAAGCCT CACTAAAAGG GTCCCTGCCT AGTTCTGTTT ACTAATCTGC 5640 CTTATTCTGT TTTTGTTCCC ATGTTAAAGA TAGAGTAAAT GCAGTATTCT CCACATAGAG 5700 ATATAGACTT CTGAAATTCT AAGATTAGAA TTATTTACAA GAAGAAGTGG GGAA 5754 4958 base pairs nucleic acid single linear DNA (genomic) 20 AGGCGGGGAC AGCAGTGCAG TGGTGGACAG AAAGCAAGTG ATCTAGGCCA GCAGCCTCCC 60 TAAAGGGACT TCAGCCCACA AAGCCAAACT TGTGGCTTTA ATACAAGCTC TGTAAATGGT 120 AAAAAAAAAA AAGTCTACAC GGACAGCAGG TATGCTCTTG CCACTGTACA GAGCAATATA 180 CAGACAAAGA GAACTGTTGA CATCTGCAGA GAAAGACCTA AGATGCTGTG GCTAAAAGAA 240 ATCAGATGGC AAATCTAACC GCCCAGGCAT CCTAAAGAGC AATGATCCTG ACAGTCTGAA 300 GACTATCAAG TTATAGACAA ATTAAGACTG GTAAAAAAAA CCCTGTATAA AATAGTAAAA 360 ACTGAAAAAA GAAAACTAGT CCTCTCATGA GAAGACAGAC CTGACATCTA CTGAAAAATA 420 GACTTTACTG GAAAAAATAT GTGTATGAAT ACCTTCTAGT TTTTGTGAAC GTTCTCAAGA 480 TGGATAAAAG CTTTTCCTTG TAAAACGAGA CTGATCAGAT AGTCATCAAG AAGATTGTTA 540 AAGAAAATTT TCCAAGGTTC GGAGTGCCAA AAGCAATAGT GTCAGATAAT GGTCCTGCCT 600 TTGTTGCCCA GGTAAGTCAG GGTGTGGCCA AGTATTTAGA GGTCAAATGA AAATTCCATT 660 GTGTGTACAG ACCTCAGAGC TCAGGAAAGA TAAAAAAGAA TAAATAAAAC TCTAAACAGA 720 CCTTGACAAA ATTAATCCTA GAGACTGGCA CAGACTTACT TGGTACTCCT TCCCCTTGCC 780 CTATTTAGAA CTGAGAATAC TCCCTCTTGA TTCGGTTTTA CTCTTTTTAA GATCCTTTAT 840 GGGGCTCCTA TGCCATCACT GTCTTAAATG ATGTGTTTAA ACCTATGTTG TTATAATAAT 900 GATCTATATG TTAAGTTAAA AGGCTTGCAG GTGGTGCAGA AAGAAGTCTG GTCACAACTG 960 GCTACAGTGA ACAAGCTGGG TACCCCAAGG ACATCTTACC AGTTCCAGCC AGAGATCTGA 1020 TCTACGATCC CCGGGTCGAC CCGGGTCGAC CCTGTGGAAT GTGTGTCAGT TAGGGTGTGG 1080 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC 1140 AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT 1200 CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC 1260 CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA 1320 GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG 1380 CTTTTGCAAA AAGCTTCACG CTGCCGCAAG CACTCAGGGC GCAAGGGCTG CTAAAGGAAG 1440 CGGAACACGT AGAAAGCCAG TCCGCAGAAA CGGTGCTGAC CCCGGATGAA TGTCAGCTAC 1500 TGGGCTATCT GGACAAGGGA AAACGCAAGC GCAAAGAGAA AGCAGGTAGC TTGCAGTGGG 1560 CTTACATGGC GATAGCTAGA CTGGGCGGTT TTATGGACAG CAAGCGAACC GGAATTGCCA 1620 GCTGGGGCGC CCTCTGGTAA GGTTGGGAAG CCCTGCAAAG TAAACTGGAT GGCTTTCTTG 1680 CCGCCAAGGA TCTGATGGCG CAGGGGATCA AGATCTGATC AAGAGACAGG ATGAGGATCG 1740 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 1800 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 1860 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 1920 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 1980 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 2040 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 2100 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 2160 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 2220 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 2280 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 2340 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT 2400 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 2460 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 2520 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 2580 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 2640 GAATCGTTTT CCGGGACGGA ATTCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA 2700 CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC 2760 TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC 2820 TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT 2880 GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT 2940 AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG 3000 ACCTACACCG AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA 3060 GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG 3120 GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA 3180 CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC 3240 AACGCCGAGA TGCGCCGCCT CGAGTACACC TGCGTCATGC TGAGACCCTC AAGCCTCACT 3300 AAAAGGGTCC CTGCCTAGTT CTGTTTACTA ATCTGCCTTA TTCTGTTTTT GTTCCCATGT 3360 TAAAGATAGA GTAAATGCAG TATTCTCCAC ATAGAGATAT AGACTTCTGA AATTCTAAGA 3420 TTAGAATTAT TTACAAGAAG AAGTGGGGAA TGAAGAATAA AAAATTACTG GCCTCTTGTG 3480 AGAACATGAA CTTTCACCTC GGAGCCCACC CCCTCCCATC TGGAAAACAT ACTTGAGAAA 3540 AACATTTTCT GGAACAACCA CAGAATGTTT CAACAGGCCA GATGTATTGC CAAACACAGG 3600 ATATGACTCT TTGGTTGAGT AAATTTGTGG TTGTTAAACT TCCCCTATTC CCTCCCCATT 3660 CCCCCTCCCA GTTTGTGGTT TTTTCCTTTA AAAGCTTGTG AAAAATTTGA GTCGTCGTCG 3720 AGACTCCTCT ACCCTGTGCA AAGGTGTATG AGTTTCGACC CCAGAGCTCT GTGTGCTTTC 3780 TGTTGCTGCT TTATTTCGAC CCCAGAGCTC TGGTCTGTGT GCTTTCATGT CGCTGCTTTA 3840 TTAAATCTTA CCTTCTACAT TTTATGTATG GTCTCAGTGT CTTCTTGGGT ACGCGGCTGT 3900 CCCGGGACTT GAGTGTCTGA GTGAGGGTCT TCCCTCGAGG GTCTTTCATT TGGTACATGG 3960 GCCGGGAATT CGAGAATCTT TCATTTGGTG CATTGGCCGG GAATTCGAAA ATCTTTCATT 4020 TGGTGCATTG GCCGGGAAAC AGCGCGACCA CCCAGAGGTC CTAGACCCAC TTAGAGGTAA 4080 GATTCTTTGT TCTGTTTTGG TCTGATGTCT GTGTTCTGAT GTCTGTGTTC TGTTTCTAAG 4140 TCTGGTGCGA TCGCAGTTTC AGTTTTGCGG ACGCTCAGTG AGACCGCGCT CCGAGAGGGA 4200 GTGCGGGGTG GATAAGGATA GACGTGTCCA GGTGTCCACC GTCCGTTCGC CCTGGGAGAC 4260 GTCCCAGGAG GAACAGGGGA GGATCAGGGA CGCCTGGTGG ACCCCTTTGA AGGCCAAGAG 4320 ACCATTTGGG GTTGCGAGAT CGTGGGTTCG AGTCCCACCT CGTGCCCAGT TGCGAGATCG 4380 TGGGTTCGAG TCCCACCTCG TGTTTTGTTG CGAGATCGTG GGTTCGAGTC CCACCTCGCG 4440 TCTGGTCACG GGATCGTGGG TTCGAGTCCC ACCTCGTGTT TTGTTGCGAG ATCGTGGGTT 4500 CGAGTCCCAC CTCGCGTCTG GTCACGGGAT CGTGGGTTCG AGTCCCACCT CGTGCAGAGG 4560 GTCTCAATTG GCCGGCCTTA GAGAGGCCAT CTGATTCTTC TGGTTTCTCT TTTTGTCTTA 4620 GTCTCGTGTC CGCTCTTGTT GTGACTACTG TTTTTCTAAA AATGGGACAA TCTGTGTCCA 4680 CTCCCCTTTC TCTGACTCTG GTTCTGTCGC TTGGTAATTT TGTTTGTTTA CGTTTGTTTT 4740 TGTGAGTCGT CTATGTTGTC TGTTACTATC TTGTTTTTGT TTGTGGTTTA CGGTTTCTGT 4800 GTGTGTCTTG TGTGTCTCTT TGTGTTCAGA CTTGGACTGA TGACTGACGA CTGTTTTTAA 4860 GTTATGCCTT CTAAAATAAG CCTAAAAATC CTGTCAGATC CCTATGCTGA CCACTTCCTT 4920 TCAGATCAAC AGCTGCCCTT ACGTATCGAT GGATCCGA 4958 7080 base pairs nucleic acid single linear DNA (genomic) 21 GAATACAAGC TTGCATGCCT GCAGGTCGAC TCTAGAGGAT CTTGAAGAAT AAAAAATTAC 60 TGGCCTCTTG TGAGAACATG AACTTTCACC TCGGAGCCCA CCCCCTCCCA TCTGGAAAAC 120 ATACTTGAGA AAAACATTTT CTGGAACAAC CACAGAATGT TTCAACAGGC CAGATGTATT 180 GCCAAACACA GGATATGACT CTTTGGTTGA GTAAATTTGT GGTTGTTAAA CTTCCCCTAT 240 TCCCTCCCCA TTCCCCCTCC CAGTTTGTGG TTTTTTCCTT TAAAAGCTTG TGAAAAATTT 300 GAGTCGTCGT CGAGACTCCT CTACCCTGTG CAAAGGTGTA TGAGTTTCGA CCCCAGAGCT 360 CTGTGTGCTT TCTGTTGCTG CTTTATTTCG ACCCCAGAGC TCTGGTCTGT GTGCTTTCAT 420 GTCGCTGCTT TATTAAATCT TACCTTCTAC ATTTTATGTA TGGTCTCAGT GTCTTCTTGG 480 GTACGCGGCT GTCCCGGGAC TTGAGTGTCT GAGTGAGGGT CTTCCCTCGA GGGTCTTTCA 540 TTTGGTACAT GGGCCGGGAA TTCGAGAATC TTTCATTTGG TGCATTGGCC GGGAATTCGA 600 AAATCTTTCA TTTGGTGCAT TGGCCGGGAA ACAGCGCGAC CACCCAGAGG TCCTAGACCC 660 ACTTAGAGGT AAGATTCTTT GTTCTGTTTT GGTCTGATGT CTGTGTTCTG ATGTCTGTGT 720 TCTGTTTCTA AGTCTGGTGC GATCGCAGTT TCAGTTTTGC GGACGCTCAG TGAGACCGCG 780 CTCCGAGAGG GAGTGCGGGG TGGATAAGGA TAGACGTGTC CAGGTGTCCA CCGTCCGTTC 840 GCCCTGGGAG ACGTCCCAGG AGGAACAGGG GAGGATCAGG GACGCCTGGT GGACCCCTTT 900 GAAGGCCAAG AGACCATTTG GGGTTGCGAG ATCGTGGGTT CGAGTCCCAC CATCGATGGT 960 GCAGAGGGTC TCAATTGGCC GGCCTTAGAA TTACGGATCT AGCATGATTG AACAAGATGG 1020 ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA 1080 ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT 1140 TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG 1200 GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA 1260 AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA 1320 CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT 1380 TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTGC 1440 TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC 1500 GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT 1560 GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT 1620 CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG 1680 TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT 1740 CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC 1800 GGGACTCTGG GGTTCGTAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC 1860 GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAG TTAGCTTGTT TCTTTACTGT 1920 TTGTCAATTC TATTATTTCA ATACAGAACA ATAGCTTCTA TAACTGAAAT ATATTTGCTA 1980 TTGTATATTA TGATTGTCCC TCGAACCATG AACACTCCTC CAGCTGAATT TCACAATTCC 2040 TCTGTCATCT GCCAGGCCAT TAAGTTATTC ATGGAAGATC TTTGAGGAAC ACTGCAAGTT 2100 CATATCATAA ACACATTTGA AATTGAGTAT TGTTTTGCAT TGTATGGAGC TATGTTTTGC 2160 TGTATCCTCA GAAAAAAAGT TTGTTATAAA GCATTCACAC CCATAAAAAG ATAGATTTAA 2220 ATATTCCAGC TATAGGAAAG AAAGTGCGTC TGCTCTTCAC TCTAGTCTCA GTTGGCTCCT 2280 TCACATGCAT GCTTCTTTAT TTCTCCTATT TTGTCAAGAA AATAATAGGT CACGTCTTGT 2340 TCTCACTTAT GTCCTGCCTA GCATGGCTCA GATGCACGTT GTAGATACAA GAAGGATCAA 2400 ATGAAACAGA CTTCTGGTCT GTTACTACAA CCATAGTAAT AAGCACACTA ACTAATAATT 2460 GCTAATTATG TTTTCCATCT CTAAGGTTCC CACATTTTTC TGTTTTCTTA AAGATCCCAT 2520 TATCTGGTTG TAACTGAAGC TCAATGGAAC ATGAGCAATA TTTCCCAGTC TTCTCTCCCA 2580 TCCAACAGTC CTGATGGATT AGCAGAACAG GCAGAAAACA CATTGTTACC CAGAATTAAA 2640 AACTAATATT TGCTCTCCAT TCAATCCAAA ATGGACCTAT TGAAACTAAA ATCTAACCCA 2700 ATCCCATTAA ATGATTTCTA TGGCGTCAAA GGTCAAACTT CTGAAGGGAA CCTGTGGGTG 2760 GGTCACAATT CAGGCTATAT ATTCCCCAGG GCTCAGCCAG TGTCTGTACA TACACAACGG 2820 ATCCTGTGGA CAGCTCACCT AGCTGCAATG GCTACAGGCT CCCGGACGTC CCTGCTCCTG 2880 GCTTTTGGCC TGCTCTGCCT GCCCTGGCTT CAAGAGGGCA GTGCCTTCCC AACCATTCCC 2940 TTATCCAGGC TTTTTGACAA CGCTATGCTC CGCGCCCATC GTCTGCACCA GCTGGCCTTT 3000 GACACCTACC AGGAGTTTGA AGAAGCCTAT ATCCCAAAGG AACAGAAGTA TTCATTCCTG 3060 CAGAACCCCC AGACCTCCCT CTGTTTCTCA GAGTCTATTC CGACACCCTC CAACAGGGAG 3120 GAAACACAAC AGAAATCCAA CCTAGAGCTG CTCCGCATCT CCCTGCTGCT CATCCAGTCG 3180 TGGCTGGAGC CCGTGCAGTT CCTCAGGAGT GTCTTCGCCA ACAGCCTGGT GTACGGCGCC 3240 TCTGACAGCA ACGTCTATGA CCTCCTAAAG GACCTAGAGG AAGGCATCCA AACGCTGATG 3300 GGGAGGCTGG AAGATGGCAG CCCCCGGACT GGGCAGATCT TCAAGCAGAC CTACAGCAAG 3360 TTCGACACAA ACTCACACAA CGATGACGCA CTACTCAAGA ACTACGGGCT GCTCTACTGC 3420 TTCAGGAAGG ACATGGACAA GGTCGAGACA TTCCTGCGCA TCGTGCAGTG CCGCTCTGTG 3480 GAGGGCAGCT GTGGCTTCTA GCTGCCCGGG TGGCATCCTG TGACCCCTCC CCAGTGCCTC 3540 TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT 3600 GCATCAAAAA AAAAAAAAAG CTAGCGGCCG CTAGACTTCT GAAATTCTAA GATTAGAATT 3660 ATTTACAAGA AGAAGTGGGG AATGAAGAAT AAAAAATTAC TGGCCTCTTG TGAGAACATG 3720 AACTTTCACC TCGGAGCCCA CCCCCTCCCA TCTGGAAAAC ATACTTGAGA AAAACATTTT 3780 CTGGAACAAC CACAGAATGT TTCAACAGGC CAGATGTATT GCCAAACACA GGATATGACT 3840 CTTTGGTTGA GTAAATTTGT GGTTGTTAAA CTTCCCCTAT TCCCTCCCCA TTCCCCCTCC 3900 CAGTTTGTGG TTTTTTCCTT TAAAAGCTTG TGAAAAATTT GAGTCGTCGT CGAGACTCCT 3960 CTACCCTGTG CAAAGGTGTA TGAGTTTCGA CCCCAGAGCT CTGTGTGCTT TCTGTTGCTG 4020 CTTTATTTCG ACCCCAGAGC TCTGGTCTGT GTGCTTTCAT GTCGCTGCTT TATTAAATCT 4080 TACCTTCTAC ATTTTATGTA TGGTCTCAGT GTCTTCTTGG GTACGCGGCT GTCCCGGGAC 4140 TTGAGTGTCT GAGTGAGGGT CTTCCCTCGA GGGTCTTTCA TTTGGTACAT GGGCCGGGAA 4200 TTCGAGAATC TTTCATTTGG TGCATTGGCC GGGAATTCGA AAATCTTTCA GATCCCCGGG 4260 TACCGAGCTC GAATTCCGGT CTCCCTATAG TGAGTCGTAT TAATTTCGAT AAGCCAGCTG 4320 CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT 4380 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC 4440 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA 4500 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT 4560 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC 4620 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT 4680 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG 4740 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG 4800 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 4860 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG 4920 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC 4980 GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA 5040 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT 5100 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT 5160 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA 5220 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC 5280 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT 5340 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA 5400 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA 5460 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA 5520 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA 5580 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG 5640 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA 5700 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT 5760 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT 5820 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA 5880 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT 5940 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA 6000 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC 6060 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG 6120 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC 6180 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 6240 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 6300 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG 6360 AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC 6420 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC 6480 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT 6540 GTACTGAGAG TGCACCATAT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC 6600 CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA AGGAGATGGC 6660 GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT 6720 GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC 6780 AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCTGGCT 6840 AGCGATGACC CTGCTGATTG GTTCGCTGAC CATTTCCGGG GTGCGGAACG GCGTTACCAG 6900 AAACTCAGAA GGTTCGTCCA ACCAAACCGA CTCTGACGGC AGTTTACGAG AGAGATGATA 6960 GGGTCTGCTT CAGTAAGCCA GATGCTACAC AATTAGGCTT GTACATATTG TCGTTAGAAC 7020 GCGGCTACAA TTAATACATA ACCTTATGTA TCATACACAT ACGATTTAGG TGACACTATA 7080 6795 base pairs nucleic acid single linear DNA (genomic) 22 AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT TTTGCAAGGC 60 ATGGAAAAAT ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTCAGGAA CAGATGGAAC 120 AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC 180 AAGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG 240 CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC AGCAGTTTCT 300 AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT 360 TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA 420 ATAAAAGAGC CCACAACCCC TCACTCGGGG CGCCAGTCCT CCGATTGACT GAGTCGCCCG 480 GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC 540 CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT CATTTGGGGG 600 CTCGTCCGGG ATCGGGAGAC CCCTGCCCAG GGACCACCGA CCCACCACCG GGAGGTAAGC 660 TGGCCAGCAA CTTATCTGTG TCTGTCCGAT TGTCTAGTGT CTATGACTGA TTTTATGCGC 720 CTGCGTCGGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC 780 GAGTTCGGAA CACCCGGCCG CAACCCTGGG AGACGTCCCA GGAGGAACAG GGGAGGATCA 840 GGGACGCCTG GTGGACCCCT TTGAAGGCCA AGAGACCATT TGGGGTTGCG AGATCGTGGG 900 TTCGAGTCCC ACCTCGTGCC CAGTTGCGAG ATCGTGGGTT CGAGTCCCAC CTCGTGTTTT 960 GTTGCGAGAT CGTGGGTTCG AGTCCCACCT CGCGTCTGGT CACGGGATCG TGGGTTCGAG 1020 TCCCACCTCG TGTTTTGTTG CGAGATCGTG GGTTCGAGTC CCACCTCGCG TCTGGTCACG 1080 GGATCGTGGG TTCGAGTCCC ACCTCGTGCA GAGGGTCTCA ATTGGCCGGC CTTAGAGAGG 1140 CCATCTGATT CTTCTGGTTT CTCTTTTTGT CTTAGTCTCG TGTCCGCTCT TGTTGTGACT 1200 ACTGTTTTTC TAAAAATGGG ACAATCTGTG TCCACTCCCC TTTCTCTGAC TCTGGTTCTG 1260 TCGCTTGGTA ATTTTGTTTG TTTACGTTTG TTTTTGTGAG TCGTCTATGT TGTCTGTTAC 1320 TATCTTGTTT TTGTTTGTGG TTTACGGTTT CTGTGTGTGT CTTGTGTGTC TCTTTGTGTT 1380 CAGACTTGGA CTGATGACTG ACGACTGTTT TTAAGTTATG CCTTCTAAAA TAAGCCTAAA 1440 AATCCTGTCA GATCCCTATG CTGACCACTT CCTTTCAGAT CAACAGCTGC CCTTACTCGA 1500 GCTCAAGCTT CGAATTCTGC AGTCGACGGT ACCGCGGCCG CTAACTAATA GCCCATTCTC 1560 CAAGGTACGT AGCGGGGATC AATTCCGCCC CCCCCCTAAC GTTACTGGCC GAAGCCGCTT 1620 GGAATAAGGC CGGTGTGCGT TTGTCTATAT GTTATTTTCC ACCATATTGC CGTCTTTTGG 1680 CAATGTGAGG GCCCGGAAAC CTGGCCCTGT CTTCTTGACG AGCATTCCTA GGGGTCTTTC 1740 CCCTCTCGCC AAAGGAATGC AAGGTCTGTT GAATGTCGTG AAGGAAGCAG TTCCTCTGGA 1800 AGCTTCTTGA AGACAAACAA CGTCTGTAGC GACCCTTTGC AGGCAGCGGA ACCCCCCACC 1860 TGGCGACAGG TGCCTCTGCG GCCAAAAGCC ACGTGTATAA GATACACCTG CAAAGGCGGC 1920 ACAACCCCAG TGCCACGTTG TGAGTTGGAT AGTTGTGGAA AGAGTCAAAT GGCTCTCCTC 1980 AAGCGTATTC AACAAGGGGC TGAAGGATGC CCAGAAGGTA CCCCATTGTA TGGGATCTGA 2040 TCTGGGGCCT CGGTGCACAT GCTTTACATG TGTTTAGTCG AGGTTAAAAA AACGTCTAGG 2100 CCCCCCGAAC CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATACGGGA TCCACCGGTC 2160 GCCACCATGG GTAAAGGAGA AGAACTTTTC ACAGGAGTTG TCCCAATTCT TGTTGAATTA 2220 GATGGTGATG TTAATGGGCA CAAATTTTCT GTCAGTGGAG AGGGTGAAGG TGATGCAACA 2280 TACGGAAAAC TTACCCTTAA ATTTATTTGC ACTACTGGAA AACTACCTGT TCCATGGCCA 2340 ACACTTGTCA CTACTTTCAC TTATGGTGTT CAATGCTTTT CAAGATACCC AGATCATATG 2400 AAACGGCATG ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA AAGAACTATA 2460 TTTTTCAAAG ATGACGGGAA CTACAAGACA CGTGCTGAAG TCAAGTTTGA AGGTGATACC 2520 CTTGTTAATA GAATCGAGTT AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTTGGA 2580 CACAAATTGG AATACAACTA TAACTCACAC AATGTATACA TCATGGCAGA CAAACAAAAG 2640 AATGGAACCA AAGTTAACTT CAAAATTAGA CACAACATTG AAGATGGAAG CGTTCAACTA 2700 GCAGACCATT ATCAACAAAA TACTCCAATT GGCGATGGCC CTGTCCTTTT ACCAGACAAC 2760 CATTACCTGT CCACACAATC TGCCCTTTCG AAAGATCCCA ACGAAAAGAG AGACCACATG 2820 GTCCTTCTTG AGTTTGTAAC AGCTGCTGGG ATTACACATG GCATGGATGA ACTATACAAG 2880 TCCGGATCTA GATAACTGTA TCGATGGATC CGAAGGCGGG GACAGCAGTG CAGTGGTGGA 2940 CAGAAAGCAA GTGATCTAGG CCAGCAGCCT CCCTAAAGGG ACTTCAGCCC ACAAAGCCAA 3000 ACTTGTGGCT TTAATACAAG CTCTGTAAAT GGTAAAAAAA AAAAAGTCTA CACGGACAGC 3060 AGGTATGCTC TTGCCACTGT ACAGAGCAAT ATACAGACAA AGAGAACTGT TGACATCTGC 3120 AGAGAAAGAC CTAAGATGCT GTGGCTAAAA GAAATCAGAT GGCAAATCTA ACCGCCCAGG 3180 CATCCTAAAG AGCAATGATC CTGACAGTCT GAAGACTATC AAGTTATAGA CAAATTAAGA 3240 CTGGTAAAAA AAACCCTGTA TAAAATAGTA AAAACTGAAA AAAGAAAACT AGTCCTCTCA 3300 TGAGAAGACA GACCTGACAT CTACTGAAAA ATAGACTTTA CTGGAAAAAA TATGTGTATG 3360 AATACCTTCT AGTTTTTGTG AACGTTCTCA AGATGGATAA AAGCTTTTCC TTGTAAAACG 3420 AGACTGATCA GATAGTCATC AAGAAGATTG TTAAAGAAAA TTTTCCAAGG TTCGGAGTGC 3480 CAAAAGCAAT AGTGTCAGAT AATGGTCCTG CCTTTGTTGC CCAGGTAAGT CAGGGTGTGG 3540 CCAAGTATTT AGAGGTCAAA TGAAAATTCC ATTGTGTGTA CAGACCTCAG AGCTCAGGAA 3600 AGATAAAAAA GAATAAATAA AACTCTAAAC AGACCTTGAC AAAATTAATC CTAGAGACTG 3660 GCACAGACTT ACTTGGTACT CCTTCCCCTT GCCCTATTTA GAACTGAGAA TACTCCCTCT 3720 TGATTCGGTT TTACTCTTTT TAAGATCCTT TATGGGGCTC CTATGCCATC ACTGTCTTAA 3780 ATGATGTGTT TAAACCTATG TTGTTATAAT AATGATCTAT ATGTTAAGTT AAAAGGCTTG 3840 CAGGTGGTGC AGAAAGAAGT CTGGTCACAA CTGGCTACAG TGAACAAGCT GGGTACCCCA 3900 AGGACATCTT ACCAGTTCCA GCCAGAGATC TGATCTACGA TCCCCGGGTC GACCCGGGTC 3960 GACCCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG 4020 AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC 4080 CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC 4140 CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG 4200 CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA 4260 GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTTC ACGCTGCCGC 4320 AAGCACTCAG GGCGCAAGGG CTGCTAAAGG AAGCGGAACA CGTAGAAAGC CAGTCCGCAG 4380 AAACGGTGCT GACCCCGGAT GAATGTCAGC TACTGGGCTA TCTGGACAAG GGAAAACGCA 4440 AGCGCAAAGA GAAAGCAGGT AGCTTGCAGT GGGCTTACAT GGCGATAGCT AGACTGGGCG 4500 GTTTTATGGA CAGCAAGCGA ACCGGAATTG CCAGCTGGGG CGCCCTCTGG TAAGGTTGGG 4560 AAGCCCTGCA AAGTAAACTG GATGGCTTTC TTGCCGCCAA GGATCTGATG GCGCAGGGGA 4620 TCAAGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 4680 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 4740 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 4800 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 4860 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 4920 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 4980 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 5040 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 5100 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 5160 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 5220 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 5280 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 5340 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 5400 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 5460 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 5520 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GGAATTCGTA 5580 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA 5640 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT 5700 GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA 5760 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT 5820 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG 5880 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG 5940 CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA 6000 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT 6060 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG 6120 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCCG AGATGCGCCG CCTCGAGAAC 6180 CCTGGCCCTA TTATTGGGTG GACTAACCAT GGGGGGAATT GCCGCTGGAA TAGGAACAGG 6240 GACTACTGCT CTAATGGCCA CTCAGCAATT CCAGCAGCTC CAAGCCGCAG TACAGGATGA 6300 TCTCAGGGAG GTTGAAAAAT CAATCTCTAA CCTAGAAAAG TCTCTCACTT CCCTGTCTGA 6360 AGTTGTCCTA CAGAATCGAA GGGGCCTAGA CTTGTTATTT CTAAAAGAAG GAGGGCTGTG 6420 TGCTGCTCTA AAAGAAGAAT GTTGCTTCTA TGCGGACCAC ACAGGACTAG TGAGAGACAG 6480 CATGGCCAAA TTGAGAGAGA GGCTTAATCA GAGACAGAAA CTGTTTGAGT CAACTCAAGG 6540 ATGGTTTGAG GGACTGTTTA ACAGATCCCC TTGGTTTACC ACCTTGATAT CTACCATTAT 6600 GGGACCCCTC ATTGTACTCC TAATGATTTT GCTCTTCGGA CCCTGCATTC TTAATCGATT 6660 AGTCCAATTT GTTAAAGACA GGATATCAGT GGTCCAGGCT CTAGTTTTGA CTCAACAATA 6720 TCACCAGCTG AAGCCTATAG AGTACGAGCC ATAGATAAAA TAAAAGATTT TATTTAGTCT 6780 CCAGAAAAAG GGGGG 6795 9093 base pairs nucleic acid single linear DNA (genomic) 23 AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT TTTGCAAGGC 60 ATGGAAAAAT ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTCAGGAA CAGATGGAAC 120 AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC 180 AAGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG 240 CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC AGCAGTTTCT 300 AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT 360 TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA 420 ATAAAAGAGC CCACAACCCC TCACTCGGGG CGCCAGTCCT CCGATTGACT GAGTCGCCCG 480 GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC 540 CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT CATTTGGGGG 600 CTCGTCCGGG ATCGGGAGAC CCCTGCCCAG GGACCACCGA CCCACCACCG GGAGGTAAGC 660 TGGCCAGCAA CTTATCTGTG TCTGTCCGAT TGTCTAGTGT CTATGACTGA TTTTATGCGC 720 CTGCGTCGGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC 780 GAGTTCGGAA CACCCGGCCG CAACCCTGGG AGACGTCCCA GGAGGAACAG GGGAGGATCA 840 GGGACGCCTG GTGGACCCCT TTGAAGGCCA AGAGACCATT TGGGGTTGCG AGATCGTGGG 900 TTCGAGTCCC ACCTCGTGCC CAGTTGCGAG ATCGTGGGTT CGAGTCCCAC CTCGTGTTTT 960 GTTGCGAGAT CGTGGGTTCG AGTCCCACCT CGCGTCTGGT CACGGGATCG TGGGTTCGAG 1020 TCCCACCTCG TGTTTTGTTG CGAGATCGTG GGTTCGAGTC CCACCTCGCG TCTGGTCACG 1080 GGATCGTGGG TTCGAGTCCC ACCTCGTGCA GAGGGTCTCA ATTGGCCGGC CTTAGAGAGG 1140 CCATCTGATT CTTCTGGTTT CTCTTTTTGT CTTAGTCTCG TGTCCGCTCT TGTTGTGATG 1200 ACTGTTTTTC TAAAAATGGG ACAATCTGTG TCCACTCCCC TTTCTCTGAC TCTGGTTCGT 1260 TCGCTTGGTA ATTTTGTTTG TTTACGTTTG TTTTTGTGAG TCGTCTATGT TGTCTGTTAC 1320 TATCTTGTTT TTGTTTGTGG TTTACGGTTT CTGTGTGTGT CTTGTGTGTC TCTTTGTGTT 1380 CAGACTTGGA CTGATGACTG ACGACTGTTT TTAAGTTATG CCTTCTAAAA TAAGCCTAAA 1440 AATCCTGTCA GATCCCTATG CTGACCACTT CCTTTCAGAT CAACAGCTGC CCTTACGTAT 1500 CGATGGATCC CTCGACTAAC TAATAGCCCA TTCTCCAAGG TCGAGCGGGA TCAATTCCGC 1560 CCCCCCCCTA ACGTTACTGG CCGAAGCCGC TTGGAATAAG GCCGGTGTGC GTTTGTCTAT 1620 ATGTTATTTT CCACCATATT GCCGTCTTTT GGCAATGTGA GGGCCCGGAA ACCTGGCCCT 1680 GTCTTCTTGA CGAGCATTCC TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT GCAAGGTCTG 1740 TTGAATGTCG TGAAGGAAGC AGTTCCTCTG GAAGCTTCTT GAAGACAAAC AACGTCTGTA 1800 GCGACCCTTT GCAGGCAGCG GAACCCCCCA CCTGGCGACA GGTGCCTCTG CGGCCAAAAG 1860 CCACGTGTAT AAGATACACC TGCAAAGGCG GCACAACCCC AGTGCCACGT TGTGAGTTGG 1920 ATAGTTGTGG AAAGAGTCAA ATGGCTCTCC TCAAGCGTAT TCAACAAGGG GCTGAAGGAT 1980 GCCCAGAAGG TACCCCATTG TATGGGATCT GATCTGGGGC CTCGGTGCAC ATGCTTTACA 2040 TGTGTTTAGT CGAGGTTAAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA CGTGGTTTTC 2100 CTTTGAAAAA CACGATAATA ATCATGGGCG CGGATCCCGT CGTTTTACAA CGTCGTGACT 2160 GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT 2220 GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG 2280 GCGAATGGCG CTTTGCCTGG TTTCCGGCAC CAGAAGCGGT GCCGGAAAGC TGGCTGGAGT 2340 GCGATCTTCC TGAGGCCGAT ACTGTCGTCG TCCCCTCAAA CTGGCAGATG CACGGTTACG 2400 ATGCGCCCAT CTACACCAAC GTAACCTATC CCATTACGGT CAATCCGCCG TTTGTTCCCA 2460 CGGAGAATCC GACGGGTTGT TACTCGCTCA CATTTAATGT TGATGAAAGC TGGCTACAGG 2520 AAGGCCAGAC GCGAATTATT TTTGATGGCG TTAACTCGGC GTTTCATCTG TGGTGCAACG 2580 GGCGCTGGGT CGGTTACGGC CAGGACAGTC GTTTGCCGTC TGAATTTGAC CTGAGCGCAT 2640 TTTTACGCGC CGGAGAAAAC CGCCTCGCGG TGATGGTGCT GCGTTGGAGT GACGGCAGTT 2700 ATCTGGAAGA TCAGGATATG TGGCGGATGA GCGGCATTTT CCGTGACGTC TCGTTGCTGC 2760 ATAAACCGAC TACACAAATC AGCGATTTCC ATGTTGCCAC TCGCTTTAAT GATGATTTCA 2820 GCCGCGCTGT ACTGGAGGCT GAAGTTCAGA TGTGCGGCGA GTTGCGTGAC TACCTACGGG 2880 TAACAGTTTC TTTATGGCAG GGTGAAACGC AGGTCGCCAG CGGCACCGCG CCTTTCGGCG 2940 GTGAAATTAT CGATGAGCGT GGTGGTTATG CCGATCGCGT CACACTACGT CTGAACGTCG 3000 AAAACCCGAA ACTGTGGAGC GCCGAAATCC CGAATCTCTA TCGTGCGGTG GTTGAACTGC 3060 ACACCGCCGA CGGCACGCTG ATTGAAGCAG AAGCCTGCGA TGTCGGTTTC CGCGAGGTGC 3120 GGATTGAAAA TGGTCTGCTG CTGCTGAACG GCAAGCCGTT GCTGATTCGA GGCGTTAACC 3180 GTCACGAGCA TCATCCTCTG CATGGTCAGG TCATGGATGA GCAGACGATG GTGCAGGATA 3240 TCCTGCTGAT GAAGCAGAAC AACTTTAACG CCGTGCGCTG TTCGCATTAT CCGAACCATC 3300 CGCTGTGGTA CACGCTGTGC GACCGCTACG GCCTGTATGT GGTGGATGAA GCCAATATTG 3360 AAACCCACGG CATGGTGCCA ATGAATCGTC TGACCGATGA TCCGCGCTGG CTACCGGCGA 3420 TGAGCGAACG CGTAACGCGA ATGGTGCAGC GCGATCGTAA TCACCCGAGT GTGATCATCT 3480 GGTCGCTGGG GAATGAATCA GGCCACGGCG CTAATCACGA CGCGCTGTAT CGCTGGATCA 3540 AATCTGTCGA TCCTTCCCGC CCGGTGCAGT ATGAAGGCGG CGGAGCCGAC ACCACGGCCA 3600 CCGATATTAT TTGCCCGATG TACGCGCGCG TGGATGAAGA CCAGCCCTTC CCGGCTGTGC 3660 CGAAATGGTC CATCAAAAAA TGGCTTTCGC TACCTGGAGA GACGCGCCCG CTGATCCTTT 3720 GCGAATACGC CCACGCGATG GGTAACAGTC TTGGCGGTTT CGCTAAATAC TGGCAGGCGT 3780 TTCGTCAGTA TCCCCGTTTA CAGGGCGGCT TCGTCTGGGA CTGGGTGGAT CAGTCGCTGA 3840 TTAAATATGA TGAAAACGGC AACCCGTGGT CGGCTTACGG CGGTGATTTT GGCGATACGC 3900 CGAACGATCG CCAGTTCTGT ATGAACGGTC TGGTCTTTGC CGACCGCACG CCGCATCCAG 3960 CGCTGACGGA AGCAAAACAC CAGCAGCAGT TTTTCCAGTT CCGTTTATCC GGGCAAACCA 4020 TCGAAGTGAC CAGCGAATAC CTGTTCCGTC ATAGCGATAA CGAGCTCCTG CACTGGATGG 4080 TGGCGCTGGA TGGTAAGCCG CTGGCAAGCG GTGAAGTGCC TCTGGATGTC GCTCCACAAG 4140 GTAAACAGTT GATTGAACTG CCTGAACTAC CGCAGCCGGA GAGCGCCGGG CAACTCTGGC 4200 TCACAGTACG CGTAGTGCAA CCGAACGCGA CCGCATGGTC AGAAGCCGGG CACATCAGCG 4260 CCTGGCAGCA GTGGCGTCTG GCGGAAAACC TCAGTGTGAC GCTCCCCGCC GCGTCCCACG 4320 CCATCCCGCA TCTGACCACC AGCGAAATGG ATTTTTGCAT CGAGCTGGGT AATAAGCGTT 4380 GGCAATTTAA CCGCCAGTCA GGCTTTCTTT CACAGATGTG GATTGGCGAT AAAAAACAAC 4440 TGCTGACGCC GCTGCGCGAT CAGTTCACCC GTGCACCGCT GGATAACGAC ATTGGCGTAA 4500 GTGAAGCGAC CCGCATTGAC CCTAACGCCT GGGTCGAACG CTGGAAGGCG GCGGGCCATT 4560 ACCAGGCCGA AGCAGCGTTG TTGCAGTGCA CGGCAGATAC ACTTGCTGAT GCGGTGCTGA 4620 TTACGACCGC TCACGCGTGG CAGCATCAGG GGAAAACCTT ATTTATCAGC CGGAAAACCT 4680 ACCGGATTGA TGGTAGTGGT CAAATGGCGA TTACCGTTGA TGTTGAAGTG GCGAGCGATA 4740 CACCGCATCC GGCGCGGATT GGCCTGAACT GCCAGCTGGC GCAGGTAGCA GAGCGGGTAA 4800 ACTGGCTCGG ATTAGGGCCG CAAGAAAACT ATCCCGACCG CCTTACTGCC GCCTGTTTTG 4860 ACCGCTGGGA TCTGCCATTG TCAGACATGT ATACCCCGTA CGTCTTCCCG AGCGAAAACG 4920 GTCTGCGCTG CGGGACGCGC GAATTGAATT ATGGCCCACA CCAGTGGCGC GGCGACTTCC 4980 AGTTCAACAT CAGCCGCTAC AGTCAACAGC AACTGATGGA AACCAGCCAT CGCCATCTGC 5040 TGCACGCGGA AGAAGGCACA TGGCTGAATA TCGACGGTTT CCATATGGGG ATTGGTGGCG 5100 ACGACTCCTG GAGCCCGTCA GTATCGGCGG AATTCCAGCT GAGCGCCGGT CGCTACCATT 5160 ACCAGTTGGT CTGGTGTCAA AAATAATAAT AACCGGGCAG GGGGGATCCG AAGGCGGGGA 5220 CAGCAGTGCA GTGGTGGACA GAAAGCAAGT GATCTAGGCC AGCAGCCTCC CTAAAGGGAC 5280 TTCAGCCCAC AAAGCCAAAC TTGTGGCTTT AATACAAGCT CTGTAAATGG TAAAAAAAAA 5340 AAAGTCTACA CGGACAGCAG GTATGCTCTT GCCACTGTAC AGAGCAATAT ACAGACAAAG 5400 AGAACTGTTG ACATCTGCAG AGAAAGACCT AAGATGCTGT GGCTAAAAGA AATCAGATGG 5460 CAAATCTAAC CGCCCAGGCA TCCTAAAGAG CAATGATCCT GACAGTCTGA AGACTATCAA 5520 GTTATAGACA AATTAAGACT GGTAAAAAAA ACCCTGTATA AAATAGTAAA AACTGAAAAA 5580 AGAAAACTAG TCCTCTCATG AGAAGACAGA CCTGACATCT ACTGAAAAAT AGACTTTACT 5640 GGAAAAAATA TGTGTATGAA TACCTTCTAG TTTTTGTGAA CGTTCTCAAG ATGGATAAAA 5700 GCTTTTCCTT GTAAAACGAG ACTGATCAGA TAGTCATCAA GAAGATTGTT AAAGAAAATT 5760 TTCCAAGGTT CGGAGTGCCA AAAGCAATAG TGTCAGATAA TGGTCCTGCC TTTGTTGCCC 5820 AGGTAAGTCA GGGTGTGGCC AAGTATTTAG AGGTCAAATG AAAATTCCAT TGTGTGTACA 5880 GACCTCAGAG CTCAGGAAAG ATAAAAAAGA ATAAATAAAA CTCTAAACAG ACCTTGACAA 5940 AATTAATCCT AGAGACTGGC ACAGACTTAC TTGGTACTCC TTCCCCTTGC CCTATTTAGA 6000 ACTGAGAATA CTCCCTCTTG ATTCGGTTTT ACTCTTTTTA AGATCCTTTA TGGGGCTCCT 6060 ATGCCATCAC TGTCTTAAAT GATGTGTTTA AACCTATGTT GTTATAATAA TGATCTATAT 6120 GTTAAGTTAA AAGGCTTGCA GGTGGTGCAG AAAGAAGTCT GGTCACAACT GGCTACAGTG 6180 AACAAGCTGG GTACCCCAAG GACATCTTAC CAGTTCCAGC CAGAGATCTG ATCTACGATC 6240 CCCGGGTCGA CCCGGGTCGA CCCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC 6300 AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG 6360 TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC 6420 AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC 6480 CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC 6540 GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA 6600 AAAGCTTCAC GCTGCCGCAA GCACTCAGGG CGCAAGGGCT GCTAAAGGAA GCGGAACACG 6660 TAGAAAGCCA GTCCGCAGAA ACGGTGCTGA CCCCGGATGA ATGTCAGCTA CTGGGCTATC 6720 TGGACAAGGG AAAACGCAAG CGCAAAGAGA AAGCAGGTAG CTTGCAGTGG GCTTACATGG 6780 CGATAGCTAG ACTGGGCGGT TTTATGGACA GCAAGCGAAC CGGAATTGCC AGCTGGGGCG 6840 CCCTCTGGTA AGGTTGGGAA GCCCTGCAAA GTAAACTGGA TGGCTTTCTT GCCGCCAAGG 6900 ATCTGATGGC GCAGGGGATC AAGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 6960 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 7020 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 7080 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 7140 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 7200 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 7260 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 7320 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 7380 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 7440 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 7500 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 7560 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA 7620 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 7680 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 7740 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC 7800 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 7860 TCCGGGACGG AATTCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGGA 7920 GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA 7980 GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC 8040 TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT 8100 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG 8160 CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC 8220 GAACTGAGAT ACCTACAGCG TGAGCATTGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG 8280 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA 8340 GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT 8400 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCCGAG 8460 ATGCGCCGCC TCGAGAACCC TGGCCCTATT ATTGGGTGGA CTAACCATGG GGGGAATTGC 8520 CGCTGGAATA GGAACAGGGA CTACTGCTCT AATGGCCACT CAGCAATTCC AGCAGCTCCA 8580 AGCCGCAGTA CAGGATGATC TCAGGGAGGT TGAAAAATCA ATCTCTAACC TAGAAAAGTC 8640 TCTCACTTCC CTGTCTGAAG TTGTCCTACA GAATCGAAGG GGCCTAGACT TGTTATTTCT 8700 AAAAGAAGGA GGGCTGTGTG CTGCTCTAAA AGAAGAATGT TGCTTCTATG CGGACCACAC 8760 AGGACTAGTG AGAGACAGCA TGGCCAAATT GAGAGAGAGG CTTAATCAGA GACAGAAACT 8820 GTTTGAGTCA ACTCAAGGAT GGTTTGAGGG ACTGTTTAAC AGATCCCCTT GGTTTACCAC 8880 CTTGATATCT ACCATTATGG GACCCCTCAT TGTACTCCTA ATGATTTTGC TCTTCGGACC 8940 CTGCATTCTT AATCGATTAG TCCAATTTGT TAAAGACAGG ATATCAGTGG TCCAGGCTCT 9000 AGTTTTGACT CAACAATATC ACCAGCTGAA GCCTATAGAG TACGAGCCAT AGATAAAATA 9060 AAAGATTTTA TTTAGTCTCC AGAAAAAGGG GGG 9093 46 base pairs nucleic acid single linear DNA (genomic) 24 GACTAACCTT GATTCCCTGG AGGCGGGGGT CTTTCATTTG GGGGCT 46 4834 base pairs nucleic acid single linear DNA (genomic) 25 TGAAGAATAA AAAATTACTG GCCTCTTGTG AGAACATGAA CTTTCACCTC GGAGCCCACC 60 CCCTCCCATC TGGAAAACAT ACTTGAGAAA AACATTTTCT GGAACAACCA CAGAATGTTT 120 CAACAGGCCA GATGTATTGC CAAACACAGG ATATGACTCT TTGGTTGAGT AAATTTGTGG 180 TTGTTAAACT TCCCCTATTC CCTCCCCATT CCCCCTCCCA GTTTGTGGTT TTTTCCTTTA 240 AAAGCTTGTG AAAAATTTGA GTCGTCGTCG AGACTCCTCT ACCCTGTGCA AAGGTGTATG 300 AGTTTCGACC CCAGAGCTCT GTGTGCTTTC TGTTGCTGCT TTATTTCGAC CCCAGAGCTC 360 TGGTCTGTGT GCTTTCATGT CGCTGCTTTA TTAAATCTTA CCTTCTACAT TTTATGTATG 420 GTCTCAGTGT CTTCTTGGGT ACGCGGCTGT CCCGGGACTT GAGTGTCTGA GTGAGGGTCT 480 TCCCTCGAGG GTCTTTCATT TGGTACATGG GCCGGGAATT CGAGAATCTT TCATTTGGTG 540 CATTGGCCGG GAATTCGAAA ATCTTTCATT TGGTGCATTG GCCGGGAAAC AGCGCGACCA 600 CCCAGAGGTC CTAGACCCAC TTAGAGGTAA GATTCTTTGT TCTGTTTTGG TCTGATGTCT 660 GTGTTCTGAT GTCTGTGTTC TGTTTCTAAG TCTGGTGCGA TCGCAGTTTC AGTTTTGCGG 720 ACGCTCAGTG AGACCGCGCT CCGAGAGGGA GTGCGGGGTG GATAAGGATA GACGTGTCCA 780 GGTGTCCACC GTCCGTTCGC CCTGGGAGAC GTCCCAGGAG GAACAGGGGA GGATCAGGGA 840 CGCCTGGTGG ACCCCTTTGA AGGCCAAGAG ACCATTTGGG GTTGCGAGAT CGTGGGTTCG 900 AGTCCCACCT CGTGCCCAGT TGCGAGATCG TGGGTTCGAG TCCCACCTCG TGTTTTGTTG 960 CGAGATCGTG GGTTCGAGTC CCACCTCGCG TCTGGTCACG GGATCGTGGG TTCGAGTCCC 1020 ACCTCGTGTT TTGTTGCGAG ATCGTGGGTT CGAGTCCCAC CTCGCGTCTG GTCACGGGAT 1080 CGTGGGTTCG AGTCCCACCT CGTGCAGAGG GTCTCAATTG GCCGGCCTTA GAGAGGCCAT 1140 CTGATTCTTC TGGTTTCTCT TTTTGTCTTA GTCTCGTGTC CGCTCTTGTT GTGACTACTG 1200 TTTTTCTAAA AATGGGACAA TCTGTGTCCA CTCCCCTTTC TCTGACTCTG GTTCTGTCGC 1260 TTGGTAATTT TGTTTGTTTA CGTTTGTTTT TGTGAGTCGT CTATGTTGTC TGTTACTATC 1320 TTGTTTTTGT TTGTGGTTTA CGGTTTCTGT GTGTGTCTTG TGTGTCTCTT TGTGTTCAGA 1380 CTTGGACTGA TGACTGACGA CTGTTTTTAA GTTATGCCTT CTAAAATAAG CCTAAAAATC 1440 CTGTCAGATC CCTATGCTGA CCACTTCCTT TCAGATCAAC AGCTGCCCTG CCTCCCACTC 1500 CAACTCCAGA GAGCAGCCAG CGGGTCACAG TGGTCCCGCC CATGAACCTG GAGCCTAGGG 1560 AAAAATGAGC TCGGAAATCC GGAGCAAATG AGGAGTGGTC CCTGAGAAGT CAGTGGCCTA 1620 AATGTTGTGG CTGCTGAAGC AAAAGAAGAG GAGGCTGTTC GAGTAGCCGG CCAAGAGCGC 1680 CGCGGGTTCC CAGGCAGCTT CTCATTCCCC TGTCCCTCCC ATCCCGTCTC TTGTTAACAG 1740 AAAAACTGCT TTCACTTTGA GATATGAGTG GCCCGATACA GCCAGCTGTG AGAGCTGTAC 1800 TCCCTTCCCT GCCCCACGTG TTTTCTCTTC TCAGGCGACC CCTCCCTGAG CTGCTGGCAG 1860 TGAGTCTGTT CTAAGCTCCA GTGAGGGAGG CATCCGCCCA CTTGGGGCTT CTGTCCAAGG 1920 TAAGGAGCAC CTGTGAGTCT AACTGCCAGG CTCTGATGGG GGTCTCGTCT CTGTGGGACT 1980 AGAAAGTGTC CCAACAATCT GACCAAGGTA ACAGGAAGTT AAGACAAAGA CAGAGACCAA 2040 AGTCAGAATC AGAGCTGTGC TGTGAGACAA AAAGATAAAA AAAATAAAAT GCTGGCCACA 2100 AAAGTCAGGA AAACTAGAAA ACTTAGATAG TACCTGGCAA CAAAAGAAAG CTTTTGGCTA 2160 AAGATCAACG TGTATACTGT AAAGAAAATG AGCACTGGGT GAGAGACTGC CCCAACAAAA 2220 AGAAGAGGAG CCCCCCTCAT GACCAAACCC TTCACCTGTT CGTGGCTAAA AGTAAAGAGA 2280 TAACAAAAGG GGTGCTAACA CAGAAGCTGA GTCCTTAAAA GAGTCCGGTG GCCTACCTGT 2340 TGAAGCAGCT AAAAAAGAGA CTGTGTTTCA TACTCCTCCA CTGACCAGTG CAAAACAAGC 2400 TAAAAAGTTC CTGGGCACTG CGGGCTTTTG CAGATTGTGG ATTCCAGGTT TTGCTGAGTT 2460 AAAGAGATAA ACAGCCCTTC GTATAGAAAA ATAAAAAACA ACCTTGGATG TCCTTGGATG 2520 CTATTGAGAC TGCCCTAATG TTGTCCCCAG CTATGGGACT CCTAGATGTG ACTGAGAACA 2580 AAGGTATTGC CAAAGAAGTT CTTACTCAGA GATTGGGACC CTGAAAAAGA CCTGTGGCAT 2640 ACTTGTAAGA AATTAGACCT GGTGGCTGTA AGATGGCCTG CTTGTCTGCA CATAGTGGCT 2700 TCTGGTCAAG GACGCAGATA AATTGACTCT GAGACAAAAC TTGGCACATG TCCTAGAAAG 2760 TGTGGTTCAG CCCCCATGAC CGATGGCTGA CTAACGCTCT TGAAAACATT ATCCAACTGT 2820 TCCCCTGACC GATGGACACA TTGTCAGAGC TTTTTTTGAC TGAACGAGTG ACCTTCGCTC 2880 CCCCTGCTAT CCTCGATCTC ACTACTGCCT GAGACTTCAC CTACTCATCA TTGTGCTGAC 2940 ATTCTGGCAG AAGAAACTCA TACTCGAAAT GATCTGAAGG ATCAGATCAG CCTTGGCCTG 3000 AGAGTTTGAG CTGGTACACG GATGGCAGTA GCCTGGAGGT TAAGGGTAAG CGGAAGGCGG 3060 GGACAGCAGT GCAGTGGTGG ACAGAAAGCA AGTGATCTAG GCCAGCAGCC TCCCTAAAGG 3120 GACTTCAGCC CACAAAGCCA AACTTGTGGC TTTAATACAA GCTCTGTAAA TGGTAAAAAA 3180 AAAAAAGTCT ACACGGACAG CAGGTATGCT CTTGCCACTG TACAGAGCAA TATACAGACA 3240 AAGAGAACTG TTGACATCTG CAGAGAAAGA CCTAAGATGC TGTGGCTAAA AGAAATCAGA 3300 TGGCAAATCT AACCGCCCAG GCATCCTAAA GAGCAATGAT CCTGACAGTC TGAAGACTAT 3360 CAAGTTATAG ACAAATTAAG ACTGGTAAAA AAAACCCTGT ATAAAATAGT AAAAACTGAA 3420 AAAAGAAAAC TAGTCCTCTC ATGAGAAGAC AGACCTGACA TCTACTGAAA AATAGACTTT 3480 ACTGGAAAAA ATATGTGTAT GAATACCTTC TAGTTTTTGT GAACGTTCTC AAGATGGATA 3540 AAAGCTTTTC CTTGTAAAAC GAGACTGATC AGATAGTCAT CAAGAAGATT GTTAAAGAAA 3600 ATTTTCCAAG GTTCGGAGTG CCAAAAGCAA TAGTGTCAGA TAATGGTCCT GCCTTTGTTG 3660 CCCAGGTAAG TCAGGGTGTG GCCAAGTATT TAGAGGTCAA ATGAAAATTC CATTGTGTGT 3720 ACAGACCTCA GAGCTCAGGA AAGATAAAAA AGAATAAATA AAACTCTAAA CAGACCTTGA 3780 CAAAATTAAT CCTAGAGACT GGCACAGACT TACTTGGTAC TCCTTCCCCT TGCCCTATTT 3840 AGAACTGAGA ATACTCCCTC TTGATTCGGT TTTACTCTTT TTAAGATCCT TTATGGGGCT 3900 CCTATGCCAT CACTGTCTTA AATGATGTGT TTAAACCTAT GTTGTTATAA TAATGATCTA 3960 TATGTTAAGT TAAAAGGCTT GCAGGTGGTG CAGAAAGAAG TCTGGTCACA ACTGGCTACA 4020 GTGAACAAGC TGGGTACCCC AAGGACATCT TACCAGTTCC AGCCAGAGAT CTGATCTACG 4080 TACACCTGCG TCATGCTGAG ACCCTCAAGC CTCACTAAAA GGGTCCCTGC CTAGTTCTGT 4140 TTACTAATCT GCCTTATTCT GTTTTTGTTC CCATGTTAAA GATAGAGTAA ATGCAGTATT 4200 CTCCACATAG AGATATAGAC TTCTGAAATT CTAAGATTAG AATTATTTAC AAGAAGAAGT 4260 GGGGAATGAA GAATAAAAAA TTACTGGCCT CTTGTGAGAA CATGAACTTT CACCTCGGAG 4320 CCCACCCCCT CCCATCTGGA AAACATACTT GAGAAAAACA TTTTCTGGAA CAACCACAGA 4380 ATGTTTCAAC AGGCCAGATG TATTGCCAAA CACAGGATAT GACTCTTTGG TTGAGTAAAT 4440 TTGTGGTTGT TAAACTTCCC CTATTCCCTC CCCATTCCCC CTCCCAGTTT GTGGTTTTTT 4500 CCTTTAAAAG CTTGTGAAAA ATTTGAGTCG TCGTCGAGAC TCCTCTACCC TGTGCAAAGG 4560 TGTATGAGTT TCGACCCCAG AGCTCTGTGT GCTTTCTGTT GCTGCTTTAT TTCGACCCCA 4620 GAGCTCTGGT CTGTGTGCTT TCATGTCGCT GCTTTATTAA ATCTTACCTT CTACATTTTA 4680 TGTATGGTCT CAGTGTCTTC TTGGGTACGC GGCTGTCCCG GGACTTGAGT GTCTGAGTGA 4740 GGGTCTTCCC TCGAGGGTCT TTCATTTGGT ACATGGGCCG GGAATTCGAG AATCTTTCAT 4800 TTGGTGCATT GGCCGGGAAT TCGAAAATCT TTCA 4834 4518 base pairs nucleic acid single linear DNA (genomic) 26 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 60 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 120 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 180 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 240 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 300 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 360 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 420 AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTACGCGTTA AGATACATTG 480 ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT 540 GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA 600 ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT 660 AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCATGA ACAGACTGTG AGGACTGAGG 720 GGCCTGAAAT GAGCCTTGGG ACTGTGAATC TAAAATACAC AAACAATTAG AATCAGTAGT 780 TTAACACATT ATACACTTAA AAATTGGATC TCCATTCGCC ATTCAGGCTG CGCAACTGTT 840 GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG 900 CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA 960 CGGCCAGTGA ATTGTAATAC GACTCACTAT AGGGCGAATT GGGTACACTT ACCTGGTACC 1020 CCACCCGGGT GGAAAATCGA TGGGCCCGCG GCCGCTCTAG AAGTACTCTC GAGAAGCTTT 1080 TTGAATTCTT TGGATCCACT AGTGTCGACC TGCAGGCGCG CGAGCTCCAG CTTTTGTTCC 1140 CTTTAGTGAG GGTTAATTTC GAGCTTGGCG TAATCAAGGT CATAGCTGTT TCCTGTGTGA 1200 AATTGTTATC CGCTCACAAT TCCACACAAT ATACGAGCCG GAAGTATAAA GTGTAAAGCC 1260 TGGGGTGCCT AATGAGTGAG CTAACTCACA GTAATTGCGG CTAGCGGATC TGACGGTTCA 1320 CTAAACCAGC TCTGCTTATA TAGACCTCCC ACCGTACACG CCTACCGCCC ATTTGCGTCA 1380 ATGGGGCGGA GTTGTTACGA CATTTTGGAA AGTCCCGTTG ATTTTGGTGC CAAAACAAAC 1440 TCCCATTGAC GTCAATGGGG TGGAGACTTG GAAATCCCCG TGAGTCAAAC CGCTATCCAC 1500 GCCCATTGAT GTACTGCCAA AACCGCATCA CCATGGTAAT AGCGATGACT AATACGTAGA 1560 TGTACTGCCA AGTAGGAAAG TCCCATAAGG TCATGTACTG GGCATAATGC CAGGCGGGCC 1620 ATTTACCGTC ATTGACGTCA ATAGGGGGCG TACTTGGCAT ATGATACACT TGATGTACTG 1680 CCAAGTGGGC AGTTTACCGT AAATACTCCA CCCATTGACG TCAATGGAAA GTCCCTATTG 1740 GCGTTACTAT GGGAACATAC GTCATTATTG ACGTCAATGG GCGGGGGTCG TTGGGCGGTC 1800 AGCCAGGCGG GCCATTTACC GTAAGTTATG TAACGCGGAA CTCCATATAT GGGCTATGAA 1860 CTAATGACCC CGTAATTGAT TACTATTAAT AACTAATGCA TGGCGGTAAT ACGGTTATCC 1920 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG 1980 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT 2040 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG 2100 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA 2160 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG 2220 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT 2280 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC 2340 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC 2400 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT 2460 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC 2520 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC 2580 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG 2640 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG 2700 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG 2760 GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT 2820 GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT 2880 GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA 2940 ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT 3000 GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA 3060 TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT 3120 TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG 3180 GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA 3240 TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC 3300 CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG CGGTCCGCCA 3360 CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG 3420 GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA 3480 GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT 3540 CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT 3600 CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG 3660 ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA 3720 ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC 3780 CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG 3840 ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG 3900 CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG 3960 CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG 4020 TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG 4080 AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA 4140 TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC 4200 GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG 4260 CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG 4320 CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC 4380 TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 4440 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 4500 CATTTCCCCG AAAAGTGC 4518 25 base pairs nucleic acid single linear DNA (genomic) 27 CTCCACATAG AGATATAGAC TTCTG 25 45 base pairs nucleic acid single linear DNA (genomic) 28 CGATCTTATT AATTAACTGG AGTTTTGAGC CCRMCCCCTC CCATC 45 5594 base pairs nucleic acid single linear DNA (genomic) 29 TGCATTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 60 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 120 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 180 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 240 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 300 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 360 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 420 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 480 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 540 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCGCGCC 600 AGTCCTCCGA TTGACTGAGT CGCCCGGGTA CCCGTGTATC CAATAAACCC TCTTGCAGTT 660 GCATCCGACT TGTGGTCTCG CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC 720 GTCAGCGGGG GTCTTTCATT TGGGGGCTCG TCCGGGATCG GGAGACCCCT GCCCAGGGAC 780 CACCGACCCA CCACCGGGAG GTAAGCTGGC CAGCAACTTA TCTGTGTCTG TCCGATTGTC 840 TAGTGTCTAT GACTGATTTT ATGCGCCTGC GTCGGTACTA GTTAGCTAAC TAGCTCTGTA 900 TCTGGCGGAC CCGTGGTGGA ACTGACGAGT TCGGAACACC CGGCCGCAAC CCTGGGAGAC 960 GTCCCAGGAG GAACAGGGGA GGATCAGGGA CGCCTGGTGG ACCCCTTTGA AGGCCAAGAG 1020 ACCATTTGGG GTTGCGAGAT CGTGGGTTCG AGTCCCACCT CGTGCCCAGT TGCGAGATCG 1080 TGGGTTCGAG TCCCACCTCG TGTTTTGTTG CGAGATCGTG GGTTCGAGTC CCACCTCGTT 1140 TCTGGTCACG GGATCGTGGG TTCGAGTCCC ACCTCGTGTT TTGTTGCGAG ATCGTGGGTT 1200 CGAGTCCCAC CTCGCGTCTG GTCACGGGAT CGTGGGTTCG AGTCCCACCT CGTGCAGAGG 1260 GTCTCAATTG GCCGGCCTTA GAGAGGCCAT CTGATTCTTC TGGTTTCTCT TTTTGTCTTA 1320 GTCTCGTGTC CGCTCTTGTT GTGACTACTG TTTTTCTAAA AATGGGACAA TCTGTGTCCA 1380 CTCCCCTTTC TCTGACTCTG GTTCTGTCGC TTGGTAATTT TGTTTGTTTA CGTTTGTTTT 1440 TGTGAGTCGT CTATGTTGTC TGTTACTATC TTGTTTTTGT TTGTGGTTTA CGGTTTCTGT 1500 GTGTGTCTTG TGTGTCTCTT TGTGTTCAGA CTTGGACTGA TGACTGACGA CTGTTTTTAA 1560 GTTATGCCTT CTAAAATAAG CCTAAAAATC CTGTCAGATC CCTATGCTGA CCACTTCCTT 1620 TCAGATCAAC AGCTGCCCTT ACGTATCGAT GGATCCCTCG ACTAACTAAT AGCCCATTCT 1680 CCAAGGTCGA GCGGGATCAA TTCCGCCCCC CCCCTAACGT TACTGGCCGA AGCCGCTTGG 1740 AATAAGGCCG GTGTGCGTTT GTCTATATGT TATTTTCCAC CATATTGCCG TCTTTTGGCA 1800 ATGTGAGGGC CCGGAAACCT GGCCCTGTCT TCTTGACGAG CATTCCTAGG GGTCTTTCCC 1860 CTCTCGCCAA AGGAATGCAA GGTCTGTTGA ATGTCGTGAA GGAAGCAGTT CCTCTGGAAG 1920 CTTCTTGAAG ACAAACAACG TCTGTAGCGA CCCTTTGCAG GCAGCGGAAC CCCCCACCTG 1980 GCGACAGGTG CCTCTGCGGC CAAAAGCCAC GTGTATAAGA TACACCTGCA AAGGCGGCAC 2040 AACCCCAGTG CCACGTTGTG AGTTGGATAG TTGTGGAAAG AGTCAAATGG CTCTCCTCAA 2100 GCGTATTCAA CAAGGGGCTG AAGGATGCCC AGAAGGTACC CCATTGTATG GGATCTGATC 2160 TGGGGCCTCG GTGCACATGC TTTACATGTG TTTAGTCGAG GTTAAAAAAA CGTCTAGGCC 2220 CCCCGAACCA CGGGGACGTG GTTTTCCTTT GAAAAACACG ATAATAATCA TGGCTACAGG 2280 CTCCCGGACG TCCCTGCTCC TGGCTTTTGG CCTGCTCTGC CTGCCCTGGC TTCAAGAGGG 2340 CAGTGCCTTC CCAACCATTC CCTTATCCAG GCTTTTTGAC AACGCTATGC TCCGCGCCCA 2400 TCGTCTGCAC CAGCTGGCCT TTGACACCTA CCAGGAGTTT GAAGAAGCCT ATATCCCAAA 2460 GGAACAGAAG TATTCATTCC TGCAGAACCC CCAGACCTCC CTCTGTTTCT CAGAGTCTAT 2520 TCCGACACCC TCCAACAGGG AGGAAACACA ACAGAAATCC AACCTAGAGC TGCTCCGCAT 2580 CTCCCTGCTG CTCATCCAGT CGTGGCTGGA GCCCGTGCAG TTCCTCAGGA GTGTCTTCGC 2640 CAACAGCCTG GTGTACGGCG CCTCTGACAG CAACGTCTAT GACCTCCTAA AGGACCTAGA 2700 GGAAGGCATC CAAACGCTGA TGGGGAGGCT GGAAGATGGC AGCCCCCGGA CTGGGCAGAT 2760 CTTCAAGCAG ACCTACAGCA AGTTCGACAC AAACTCACAC AACGATGACG CACTACTCAA 2820 GAACTACGGG CTGCTCTACT GCTTCAGGAA GGACATGGAC AAGGTCGAGA CATTCCTGCG 2880 CATCGTGCAG TGCCGCTCTG TGGAGGGCAG CTGTGGCTTC TAGCTGCCCG GGTGGCATCC 2940 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 3000 CTTGTCCTAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA 3060 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC 3120 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC 3180 TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT 3240 GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA 3300 AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTTCAC GCTGCCGCAA 3360 GCACTCAGGG CGCAAGGGCT GCTAAAGGAA GCGGAACACG TAGAAAGCCA GTCCGCAGAA 3420 ACGGTGCTGA CCCCGGATGA ATGTCAGCTA CTGGGCTATC TGGACAAGGG AAAACGCAAG 3480 CGCAAAGAGA AAGCAGGTAG CTTGCAGTGG GCTTACATGG CGATAGCTAG ACTGGGCGGT 3540 TTTATGGACA GCAAGCGAAC CGGAATTGCC AGCTGGGGCG CCCTCTGGTA AGGTTGGGAA 3600 GCCCTGCAAA GTAAACTGGA TGGCTTTCTT GCCGCCAAGG ATCTGATGGC GCAGGGGATC 3660 AAGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA 3720 CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC 3780 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT 3840 TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC 3900 GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG 3960 AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC 4020 TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC 4080 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT 4140 GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC 4200 CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA 4260 TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA 4320 CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT 4380 TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC 4440 TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT 4500 CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTCC AGAAAAAGGG GGGAATGAAA 4560 GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA GGCATGGAAA 4620 AATACATAAC TGAGAATAGA GAAGTTCAGA TCAAGGTCAG GAACAGATGG AACAGCTGAA 4680 TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG GCCAAGAACA 4740 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC 4800 TCAGGGCCAA GAACAGATGG TCCCCAGATG CGGTCCAGCC CTCAGCAGTT TCTAGAGAAC 4860 CATCAGATGT TTCCAGGGTG CCCCAAGGAC CTGAAATGAC CCTGTGCCTT ATTTGAACTA 4920 ACCAATCAGT TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC TCAATAAAAG 4980 AGCCCACAAC CCCTCACTCG GGGCGCCAGT AATCTGCTGC TTGCAAACAA AAAAACCACC 5040 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 5100 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA 5160 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 5220 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 5280 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 5340 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC 5400 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 5460 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 5520 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 5580 CAGCAACGCC GAGA 5594 6561 base pairs nucleic acid single linear DNA (genomic) 30 GATCCCCGGG TCGACCCGGG TCGACCCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT 60 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA 120 GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT 180 AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT 240 CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG 300 CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT 360 GCAAAAAGCT TCACGCTGCC GCAAGCACTC AGGGCGCAAG GGCTGCTAAA GGAAGCGGAA 420 CACGTAGAAA GCCAGTCCGC AGAAACGGTG CTGACCCCGG ATGAATGTCA GCTACTGGGC 480 TATCTGGACA AGGGAAAACG CAAGCGCAAA GAGAAAGCAG GTAGCTTGCA GTGGGCTTAC 540 ATGGCGATAG CTAGACTGGG CGGTTTTATG GACAGCAAGC GAACCGGAAT TGCCAGCTGG 600 GGCGCCCTCT GGTAAGGTTG GGAAGCCCTG CAAAGTAAAC TGGATGGCTT TCTTGCCGCC 660 AAGGATCTGA TGGCGCAGGG GATCAAGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG 720 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT 780 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC 840 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT 900 GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT 960 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA 1020 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT 1080 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG 1140 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA 1200 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA 1260 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA 1320 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA 1380 CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT 1440 CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT 1500 TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC 1560 CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC 1620 GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC 1680 GCCCACCCCG GAATTCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT 1740 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG 1800 AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA 1860 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG 1920 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA 1980 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC 2040 CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA 2100 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC 2160 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG 2220 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCCGA 2280 GATGCGCCGC CTCGAGTACA CCTGCGTCAT GCTGAGACCC TCAAGCCTCA CTAAAAGGGT 2340 CCCTGCCTAG TTCTGTTTAC TAATCTGCCT TATTCTGTTT TTGTTCCCAT GTTAAAGATA 2400 GAGTAAATGC AGTATTCTCC ACATAGAGAT ATAGACTTCT GAAATTCTAA GATTAGAATT 2460 ATTTACAAGA AGAAGTGGGG AATGAAGAAT AAAAAATTAC TGGCCTCTTG TGAGAACATG 2520 AACTTTCACC TCGGAGCCCA CCCCCTCCCA TCTGGAAAAC ATACTTGAGA AAAACATTTT 2580 CTGGAACAAC CACAGAATGT TTCAACAGGC CAGATGTATT GCCAAACACA GGATATGACT 2640 CTTTGGTTGA GTAAATTTGT GGTTGTTAAA CTTCCCCTAT TCCCTCCCCA TTCCCCCTCC 2700 CAGTTTGTGG TTTTTTCCTT TAAAAGCTTG TGAAAAATTT GAGTCGTCGT CGAGACTCCT 2760 CTACCCTGTG CAAAGGTGTA TGAGTTTCGA CCCCAGAGCT CTGTGTGCTT TCTGTTGCTG 2820 CTTTATTTCG ACCCCAGAGC TCTGGTCTGT GTGCTTTCAT GTCGCTGCTT TATTAAATCT 2880 TACCTTCTAC ATTTTATGTA TGGTCTCAGT GTCTTCTTGG GTACGCGGCT GTCCCGGGAC 2940 TTGAGTGTCT GAGTGAGGGT CTTCCCTCGA GGGTCTTTCA TTTGGTACAT GGGCCGGGAA 3000 TTCGAGAATC TTTCATTTGG TGCATTGGCC GGGAATTCGA AAATCTTTCA TTTGGTGCAT 3060 TGGCCGGGAA ACAGCGCGAC CACCCAGAGG TCCTAGACCC ACTTAGAGGT AAGATTCTTT 3120 GTTCTGTTTT GGTCTGATGT CTGTGTTCTG ATGTCTGTGT TCTGTTTCTA AGTCTGGTGC 3180 GATCGCAGTT TCAGTTTTGC GGACGCTCAG TGAGACCGCG CTCCGAGAGG GAGTGCGGGG 3240 TGGATAAGGA TAGACGTGTC CAGGTGTCCA CCGTCCGTTC GCCCTGGGAG ACGTCCCAGG 3300 AGGAACAGGG GAGGATCAGG GACGCCTGGT GGACCCCTTT GAAGGCCAAG AGACCATTTG 3360 GGGTTGCGAG ATCGTGGGTT CGAGTCCCAC CTCGTGCCCA GTTGCGAGAT CGTGGGTTCG 3420 AGTCCCACCT CGTGTTTTGT TGCGAGATCG TGGGTTCGAG TCCCACCTCG CGTCTGGTCA 3480 CGGGATCGTG GGTTCGAGTC CCACCTCGTG TTTTGTTGCG AGATCGTGGG TTCGAGTCCC 3540 ACCTCGCGTC TGGTCACGGG ATCGTGGGTT CGAGTCCCAC CTCGTGCAGA GGGTCTCAAT 3600 TGGCCGGCCT TAGAGAGGCC ATCTGATTCT TCTGGTTTCT CTTTTTGTCT TAGTCTCGTG 3660 TCCGCTCTTG TTGTGACTAC TGTTTTTCTA AAAATGGGAC AATCTGTGTC CACTCCCCTT 3720 TCTCTGACTC TGGTTCTGTC GCTTGGTAAT TTTGTTTGTT TACGTTTGTT TTTGTGAGTC 3780 GTCTATGTTG TCTGTTACTA TCTTGTTTTT GTTTGTGGTT TACGGTTTCT GTGTGTGTCT 3840 TGTGTGTCTC TTTGTGTTCA GACTTGGACT GATGACTGAC GACTGTTTTT AAGTTATGCC 3900 TTCTAAAATA AGCCTAAAAA TCCTGTCAGA TCCCTATGCT GACCACTTCC TTTCAGATCA 3960 ACAGCTGCCC TGCCTCCCAC TCCAACTCCA GAGAGCAGCC AGCGGGTCAC AGTGGTCCCG 4020 CCCATGAACC TGGAGCCTAG GGAAAAATGA GCTCGGAAAT CCGGAGCAAA TGAGGAGTGG 4080 TCCCTGAGAA GTCAGTGGCC TAAATGTTGT GGCTGCTGAA GCAAAAGAAG AGGAGGCTGT 4140 TCGAGTAGCC GGCCAAGAGC GCCGCGGGTT CCCAGGCAGC TTCTCATTCC CCTGTCCCTC 4200 CCATCCCGTC TCTTGTTAAC AGAAAAACTG CTTTCACTTT GAGATATGAG TGGCCCGATA 4260 CAGCCAGCTG TGAGAGCTGT ACTCCCTTCC CTGCCCCACG TGTTTTCTCT TCTCAGGCGA 4320 CCCCTCCCTG AGCTGCTGGC AGTGAGTCTG TTCTAAGCTC CAGTGAGGGA GGCATCCGCC 4380 CACTTGGGGC TTCTGTCCAA GGTAAGGAGC ACCTGTGAGT CTAACTGCCA GGCTCTGATG 4440 GGGGTCTCGT CTCTGTGGGA CTAGAAAGTG TCCCAACAAT CTGACCAAGG TAACAGGAAG 4500 TTAAGACAAA GACAGAGACC AAAGTCAGAA TCAGAGCTGT GCTGTGAGAC AAAAAGATAA 4560 AAAAAATAAA ATGCTGGCCA CAAAAGTCAG GAAAACTAGA AAACTTAGAT AGTACCTGGC 4620 AACAAAAGAA AGCTTTTGGC TAAAGATCAA CGTGTATACT GTAAAGAAAA TGAGCACTGG 4680 GTGAGAGACT GCCCCAACAA AAAGAAGAGG AGCCCCCCTC ATGACCAAAC CCTTCACCTG 4740 TTCGTGGCTA AAAGTAAAGA GATAACAAAA GGGGTGCTAA CACAGAAGCT GAGTCCTTAA 4800 AAGAGTCCGG TGGCCTACCT GTTGAAGCAG CTAAAAAAGA GACTGTGTTT CATACTCCTC 4860 CACTGACCAG TGCAAAACAA GCTAAAAAGT TCCTGGGCAC TGCGGGCTTT TGCAGATTGT 4920 GGATTCCAGG TTTTGCTGAG TTAAAGAGAT AAACAGCCCT TCGTATAGAA AAATAAAAAA 4980 CAACCTTGGA TGTCCTTGGA TGCTATTGAG ACTGCCCTAA TGTTGTCCCC AGCTATGGGA 5040 CTCCTAGATG TGACTGAGAA CAAAGGTATT GCCAAAGAAG TTCTTACTCA GAGATTGGGA 5100 CCCTGAAAAA GACCTGTGGC ATACTTGTAA GAAATTAGAC CTGGTGGCTG TAAGATGGCC 5160 TGCTTGTCTG CACATAGTGG CTTCTGGTCA AGGACGCAGA TAAATTGACT CTGAGACAAA 5220 ACTTGGCACA TGTCCTAGAA AGTGTGGTTC AGCCCCCATG ACCGATGGCT GACTAACGCT 5280 CTTGAAAACA TTATCCAACT GTTCCCCTGA CCGATGGACA CATTGTCAGA GCTTTTTTTG 5340 ACTGAACGAG TGACCTTCGC TCCCCCTGCT ATCCTCGATC TCACTACTGC CTGAGACTTC 5400 ACCTACTCAT CATTGTGCTG ACATTCTGGC AGAAGAAACT CATACTCGAA ATGATCTGAA 5460 GGATCAGATC AGCCTTGGCC TGAGAGTTTG AGCTGGTACA CGGATGGCAG TAGCCTGGAG 5520 GTTAAGGGTA AGCGGAAGGC GGGGACAGCA GTGCAGTGGT GGACAGAAAG CAAGTGATCT 5580 AGGCCAGCAG CCTCCCTAAA GGGACTTCAG CCCACAAAGC CAAACTTGTG GCTTTAATAC 5640 AAGCTCTGTA AATGGTAAAA AAAAAAAAGT CTACACGGAC AGCAGGTATG CTCTTGCCAC 5700 TGTACAGAGC AATATACAGA CAAAGAGAAC TGTTGACATC TGCAGAGAAA GACCTAAGAT 5760 GCTGTGGCTA AAAGAAATCA GATGGCAAAT CTAACCGCCC AGGCATCCTA AAGAGCAATG 5820 ATCCTGACAG TCTGAAGACT ATCAAGTTAT AGACAAATTA AGACTGGTAA AAAAAACCCT 5880 GTATAAAATA GTAAAAACTG AAAAAAGAAA ACTAGTCCTC TCATGAGAAG ACAGACCTGA 5940 CATCTACTGA AAAATAGACT TTACTGGAAA AAATATGTGT ATGAATACCT TCTAGTTTTT 6000 GTGAACGTTC TCAAGATGGA TAAAAGCTTT TCCTTGTAAA ACGAGACTGA TCAGATAGTC 6060 ATCAAGAAGA TTGTTAAAGA AAATTTTCCA AGGTTCGGAG TGCCAAAAGC AATAGTGTCA 6120 GATAATGGTC CTGCCTTTGT TGCCCAGGTA AGTCAGGGTG TGGCCAAGTA TTTAGAGGTC 6180 AAATGAAAAT TCCATTGTGT GTACAGACCT CAGAGCTCAG GAAAGATAAA AAAGAATAAA 6240 TAAAACTCTA AACAGACCTT GACAAAATTA ATCCTAGAGA CTGGCACAGA CTTACTTGGT 6300 ACTCCTTCCC CTTGCCCTAT TTAGAACTGA GAATACTCCC TCTTGATTCG GTTTTACTCT 6360 TTTTAAGATC CTTTATGGGG CTCCTATGCC ATCACTGTCT TAAATGATGT GTTTAAACCT 6420 ATGTTGTTAT AATAATGATC TATATGTTAA GTTAAAAGGC TTGCAGGTGG TGCAGAAAGA 6480 AGTCTGGTCA CAACTGGCTA CAGTGAACAA GCTGGGTACC CCAAGGACAT CTTACCAGTT 6540 CCAGCCAGAG ATCTGATCTA C 6561 55 base pairs nucleic acid single linear DNA (genomic) 31 GACTAACCTT GATTCCACTG GAGCCGTATT ACCGCCATGC ATTAGTTATT AATAG 55 47 base pairs nucleic acid single linear DNA (genomic) 32 GACTAACCTT GATTCCACTG GAGTAATTGC GGCTAGCGGA TCTGACG 47 66 base pairs nucleic acid single linear DNA (genomic) 33 GACTAACCTT GATTCCACTG GAGACACTTG ACCTCTACCG CGCCAGTCCT CCGATTGACT 60 GAGTCG 66 48 base pairs nucleic acid single linear DNA (genomic) 34 GACTAACCTT GATTCCACTG GAGGGATCCG CGCCCATGAT TATTATCG 48 55 base pairs nucleic acid single linear DNA (genomic) 35 GACTAACCTT GATTCCAGCA ATGTCATGGC TACAGGCTCC CGGACGTCCC TGCTC 55 48 base pairs nucleic acid single linear DNA (genomic) 36 GACTAACCTT GATTCCAGCA ATGTTAGGAC AAGGCTGGTG GGCACTGG 48 49 base pairs nucleic acid single linear DNA (genomic) 37 GACTAACCTT GATTCCACTG GAGGGTCGAC CCTGTGGAAT GTGTGTCAG 49 48 base pairs nucleic acid single linear DNA (genomic) 38 GACTAACCTT GATTCCACTG GAGAATCTCG TGATGGCAGG TTGGGCGT 48 54 base pairs nucleic acid single linear DNA (genomic) 39 GACTAACCTT GATTCCACTG AAGAGATTTT ATTTAGTCTC CAGAAAAAGG GGGG 54 50 base pairs nucleic acid single linear DNA (genomic) 40 GACTAACCTT GATTCCACTG AAGCCCCCAA ATGAAAGACC CCCGCTGACG 50 49 base pairs nucleic acid single linear DNA (genomic) 41 GACTAACCTT GATTCCACTG GAGCCGGGAC GGAATTCGTA ATCTGCTGC 49 47 base pairs nucleic acid single linear DNA (genomic) 42 GACTAACCTT GATTCCACTG GAGTTCTCGA GGCGGCGCAT CTCGGCG 47 20 base pairs nucleic acid single linear DNA (genomic) 43 CGCTCTAGAA CTAGTGGATC 20 21 base pairs nucleic acid single linear DNA (genomic) 44 GTAATACGAC TCACTATAGG G 21 43 base pairs nucleic acid single linear DNA (genomic) 45 CGATCCACTG GAGCTCGGAG CCCACCCCCT CCCATCTAGA GGT 43 43 base pairs nucleic acid single linear DNA (genomic) 46 CGTCCTCCTG GAGAGCACAG GGTAGAGGAG TCTCGACGGT CAG 43 43 base pairs nucleic acid single linear DNA (genomic) 47 CGCAACCCTG GAGACCTCTA GATGGGAGGG GGTGGGCTCC GAG 43 43 base pairs nucleic acid single linear DNA (genomic) 48 GCAGGACCTG GAGCTGACCG TCGAGACTCC TCTACCCTGT GCT 43 20 base pairs nucleic acid single linear DNA (genomic) 49 CGCTCTAGAA CTAGTGGATC 20 21 base pairs nucleic acid single linear DNA (genomic) 50 GTAATACGAC TCACTATAGG G 21 19 base pairs nucleic acid single linear DNA (genomic) 51 TACGTATCGA TGGATCCGA 19 19 base pairs nucleic acid single linear DNA (genomic) 52 GGATCCATCG ATACGTAAG 19 38 base pairs nucleic acid single linear DNA (genomic) 53 GGCCGCTAAC TAATAGCCCA TTCTCCAAGG TACGTAGC 38 38 base pairs nucleic acid single linear DNA (genomic) 54 TACGTACCTT GGAGAATGGG CTATTAGTTA GCGGCCGC 38 55 base pairs nucleic acid single linear DNA (genomic) 55 GACTAACCTT GATTCCACTG GAGTTTTCTC TATTCTTCAT TCCCCACTTC TTCTT 55 60 base pairs nucleic acid single linear DNA (genomic) 56 GACTAACCTT GATTCCACTG GAGAATCTGG ACCAATTCTA TATAAGCCTG TGAAAAATTT 60 46 base pairs nucleic acid single linear DNA (genomic) 57 GACTAACCTT GATTCCACTG GAGAAGAAGA AGTGGGGAAT GAAGAA 46 51 base pairs nucleic acid single linear DNA (genomic) 58 GACTAACCTT GATTCCACTG GAGATCTCTA GATGGGAGGG GGTCTGGGCT C 51 47 base pairs nucleic acid single linear DNA (genomic) 59 GACTAACCTT GATTCCACTG GAGCTCGGAG CCCACCCCCT CCCATCT 47 47 base pairs nucleic acid single linear DNA (genomic) 60 GACTAACCTT GATTCCACTG GAGGGAGGCC CTTATCTCAA AAATGTT 47 51 base pairs nucleic acid single linear DNA (genomic) 61 GACTAACCTT GATTCCACTG GAGTCTAAGA ACATTTTTGA GATAAGGGCC T 51 44 base pairs nucleic acid single linear DNA (genomic) 62 GACTAACCTT GATTCCACTG GAGTCACAGG CTTATATAGT GAAA 44 46 base pairs nucleic acid single linear DNA (genomic) 63 GACTAACCTT GATTCCCTGG AGACTGCACT GCTGTCCCCG CCTTCG 46 53 base pairs nucleic acid single linear DNA (genomic) 64 GAGTAACCTT GATTCCCTGG AGATTTCTCA GACCCGGGTC GACCCTGTGG AAT 53 44 base pairs nucleic acid single linear DNA (genomic) 65 GACTAACCTT GATTCCCTGG AGCTCGAGGC GGCGCATCTC GGCG 44 47 base pairs nucleic acid single linear DNA (genomic) 66 GACTAACCTT GATTCCCTGA AGACCTGCGT CATGCTGAGA CCCTCAA 47 50 base pairs nucleic acid single linear DNA (genomic) 67 GACTAACCTT GATTCCCTGA AGCGGCCAAT GCACCAAATG AAAGATTTTC 50 50 base pairs nucleic acid single linear DNA (genomic) 68 CGCATCTTTT AATTAACTGG AGARAATTTT TYACAGGCTT ATATAGKAAA 50 49 base pairs nucleic acid single linear DNA (genomic) 69 NNNNNNNNNN NNNNNNCTGG AGTTTTGAGC CCACCCCCTC CCATCTGGA 49 41 base pairs nucleic acid single linear DNA (genomic) 70 TGAACTTTCA CCTCGGAGCC CACCCCCTCC CATCTGGAAA A 41 32 base pairs nucleic acid single linear DNA (genomic) 71 TTTTTCCTTT AAAAGCTTGT GAAAAATTTG AG 32 32 base pairs nucleic acid single linear DNA (genomic) 72 CTCAAATTTT TCACAAGCTT TTAAAGGAAA AA 32 41 base pairs nucleic acid single linear DNA (genomic) 73 ACTTGAAAGT GGAGCCTCGG GTGGGGGAGG GTAGACCTTT T 41 50 base pairs nucleic acid single linear DNA (genomic) 74 NNNNNNNNNN NNNNNNCTGG AGAAAATTTT TCACAGGCTT ATATAGGAAA 50 

What is claimed is:
 1. A method for isolating and identifying promoters comprising the steps of: a) providing a vector comprising a portion of a promoter region from a retrovirus transposon Long Terminal Repeat (LTR) and having two non-complementary overhanging termini, said vector being generated by digesting the vector with one or more class IIS restriction enzymes, creating non-complementary termini for directional joining of promoter sequences with the vector; b) designing two PCR primers to amplify a region of a retrovirus transposon LTR promoter from template nucleic acid to produce a nucleic acid fragment, wherein said primers comprise at least one predetermined restriction endonuclease recognition site that recognizes a restriction endonuclease that cleaves at a distance from the recognition site and creates non-palindromic overhanging termini, a sequence complementary to the template nucleic acid, and bases positioned at the restriction endonuclease cleavage site that form non-palindromic overhanging termini after cleavage by the restriction endonuclease and are selected to be complementary to only one of the overhanging termini of the vector; c) combining the primers with template nucleic acid and performing a polymerase chain reaction to produce multiple copies of the template nucleic acid to form amplified template fragments incorporating the restriction endonuclease recognition site; d) digesting the amplified template fragments with one or more restriction endonucleases that recognize the restriction endonuclease recognition site of the primers to create digested template fragments having non-palindromic overhanging termini that are complementary to the respective termini of the vector; e) combining the digested template fragments in a ligation reaction with the vector to produce a gene vector with an intact LTR sequence; f) transforming the vector with an intact LTR sequence into at least one cell type; and g) selecting for expression from the LTR sequence in said cell type; whereby expression from the LTR sequence identifies a promoter.
 2. The method of claim 1 wherein the template nucleic acid is DNA or RNA.
 3. The method of claim 1 further comprising the step of sequencing the amplified template fragments to identify the promoter.
 4. A promoter sequence comprising one of SEQ ID NOS:3-13 identified using the method of claim
 1. 5. The method of claim 1, wherein said cell type is a retroviral vector producer cell.
 6. A vector having the sequence of SEQ ID NO:1, wherein the vector is prepared for promoter insertion by digesting with the restriction enzyme BpmI, to produce a larger DNA fragment and a smaller DNA fragment, and by removing or inactivating the smaller DNA fragment.
 7. A method for creating, isolating and identifying useful promoters comprising the steps of: a) providing a vector comprising a portion of a promoter region from a retrovirus transposon LTR and having two non-complementary, non-palindromic overhanging termini; b) providing a pool of related but non-identical template LTR nucleic acid sequences; c) providing two primers for amplification of each of a series of two or more contiguous regions of said template LTR sequences, wherein the primers contain class IIS restriction enzyme recognition sites that are recognized by the respective restriction enzyme, and which sites are positioned within the primers to permit digestion by said respective restriction enzyme within the LTR sequences, wherein the termini of the digested contiguous regions are non-palindromic and complementary to the termini of only the adjacent fragments when those fragments are similarly digested; d) performing polymerase chain reactions to form amplified adjacent fragments incorporating terminal restriction endonuclease sites; e) digesting the amplified fragments with one or more class IIS restriction endonuclease enzymes that recognize the respective sites on the fragments, creating two overhanging termini on each fragment, wherein the termini of the digested contiguous regions are non-palindromic and complementary to the termini of only the adjacent fragments when those fragments are similarly digested; f) purifying the digested nucleic acid fragments; g) combining the digested nucleic acid fragments with said vector in a ligation reaction to produce an intact LTR sequence; h) transforming the assembled vector and fragments into at least one cell type; and i) selecting for expression from the LTR sequence of the vector in said cell type; whereby expression from the LTR sequence identifies a promoter.
 8. The method of claim 7, wherein said cell type is a retroviral vector producer cell.
 9. The method of claim 5, wherein the retroviral producer cell is used to preferentially transmit and select for vector DNA sequences containing promoters that are actively expressed in said retroviral producer cell.
 10. The method of claim 8, wherein the retroviral vector producer cell is used to preferentially transmit and select for vector DNA sequences containing promoters that are actively expressed in said retroviral producer cell. 